Thermoresponsive micropatterned substrates for single cell studies.

We describe the design of micropatterned surfaces for single cell studies, based on thermoresponsive polymer brushes. We show that brushes made of poly(N-isopropylacrylamide) grafted at high surface density display excellent protein and cell anti-adhesive properties. Such brushes are readily pattern...

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Autores principales:	Kalpana Mandal, Martial Balland, Lionel Bureau
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16a18517c32044e78775879dcf4c6339
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spelling	oai:doaj.org-article:16a18517c32044e78775879dcf4c63392021-11-18T07:16:37ZThermoresponsive micropatterned substrates for single cell studies.1932-620310.1371/journal.pone.0037548https://doaj.org/article/16a18517c32044e78775879dcf4c63392012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22701519/?tool=EBIhttps://doaj.org/toc/1932-6203We describe the design of micropatterned surfaces for single cell studies, based on thermoresponsive polymer brushes. We show that brushes made of poly(N-isopropylacrylamide) grafted at high surface density display excellent protein and cell anti-adhesive properties. Such brushes are readily patterned at the micron scale via deep UV photolithography. A proper choice of the adhesive pattern shapes, combined with the temperature-dependent swelling properties of PNIPAM, allow us to use the polymer brush as a microactuator which induces cell detachment when the temperature is reduced below [Formula: see text]C.Kalpana MandalMartial BallandLionel BureauPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37548 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kalpana Mandal Martial Balland Lionel Bureau Thermoresponsive micropatterned substrates for single cell studies.
description	We describe the design of micropatterned surfaces for single cell studies, based on thermoresponsive polymer brushes. We show that brushes made of poly(N-isopropylacrylamide) grafted at high surface density display excellent protein and cell anti-adhesive properties. Such brushes are readily patterned at the micron scale via deep UV photolithography. A proper choice of the adhesive pattern shapes, combined with the temperature-dependent swelling properties of PNIPAM, allow us to use the polymer brush as a microactuator which induces cell detachment when the temperature is reduced below [Formula: see text]C.
format	article
author	Kalpana Mandal Martial Balland Lionel Bureau
author_facet	Kalpana Mandal Martial Balland Lionel Bureau
author_sort	Kalpana Mandal
title	Thermoresponsive micropatterned substrates for single cell studies.
title_short	Thermoresponsive micropatterned substrates for single cell studies.
title_full	Thermoresponsive micropatterned substrates for single cell studies.
title_fullStr	Thermoresponsive micropatterned substrates for single cell studies.
title_full_unstemmed	Thermoresponsive micropatterned substrates for single cell studies.
title_sort	thermoresponsive micropatterned substrates for single cell studies.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/16a18517c32044e78775879dcf4c6339
work_keys_str_mv	AT kalpanamandal thermoresponsivemicropatternedsubstratesforsinglecellstudies AT martialballand thermoresponsivemicropatternedsubstratesforsinglecellstudies AT lionelbureau thermoresponsivemicropatternedsubstratesforsinglecellstudies
_version_	1718423645829726208

Thermoresponsive micropatterned substrates for single cell studies.

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