Recovery of Lignocellulolytic Enzymes and Valorization of Spent Mushroom Substrate

Spent Mushroom Substrate (SMS) comprises sugarcane bagasse, coconut coir, chicken manure, and paddy straw; inoculated with and farmed for Agaricus bisporus. At present, the waste generation at a mushroom cultivation plant in Goa is 40 tons/day (15,000 tons annually). Valorization of this waste has b...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anumeha Vats, Anuj Sangam Kurade, Srikanth Mutnuri
Formato:	article
Lenguaje:	EN
Publicado:	Mahidol University 2021
Materias:	spent mushroom substrate lignocellulolytic enzymes laccase cellulase dye decolorization briquette Environmental technology. Sanitary engineering TD1-1066 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/16a2ffa50a704249a40aac9dad96ab21
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:16a2ffa50a704249a40aac9dad96ab21
record_format	dspace
spelling	oai:doaj.org-article:16a2ffa50a704249a40aac9dad96ab212021-12-03T08:50:39ZRecovery of Lignocellulolytic Enzymes and Valorization of Spent Mushroom Substrate10.32526/ennrj/20/2021000991686-54562408-2384https://doaj.org/article/16a2ffa50a704249a40aac9dad96ab212021-11-01T00:00:00Zhttps://ph02.tci-thaijo.org/index.php/ennrj/article/view/245148/166226https://doaj.org/toc/1686-5456https://doaj.org/toc/2408-2384Spent Mushroom Substrate (SMS) comprises sugarcane bagasse, coconut coir, chicken manure, and paddy straw; inoculated with and farmed for Agaricus bisporus. At present, the waste generation at a mushroom cultivation plant in Goa is 40 tons/day (15,000 tons annually). Valorization of this waste has been explored in terms of extracting lignocellulolytic enzymes and briquette production. SMS was screened for the presence of lignocellulolytic enzymes and then was used to make briquettes. The enzymes found in SMS were cellulase and laccase, which were further concentrated via tangential flow filtration (TFF). Enzyme activity for Cellulase increased by four-fold (from 255.34±1.30 U/mL increased to 1022.21±4.84 U/mL) and Laccase increased by three-fold (from 4.83±0.02 U/mL to 13.21±0.05 U/mL). The concentrated enzyme cocktail was used to decolorize congo red dye. After only eight hours of enzymatic treatment at pH 4.8 on congo red, approx. 40-49% decolorization was accomplished. The color removal was due to the presence of the laccase enzyme. After enzyme extraction, all the residual SMS was utilized to generate briquettes with an initial reduction in its moisture content from 50% to 10%. The resulting briquette gave a Gross Calorific Value of 4,143 Kcal/kg with 12.60% ash content. Thus, SMS proves to be a valuable source for recovering enzymes and a cost-effective material for briquette production rather than going into landfills.Anumeha VatsAnuj Sangam KuradeSrikanth MutnuriMahidol Universityarticlespent mushroom substratelignocellulolytic enzymeslaccasecellulasedye decolorizationbriquetteEnvironmental technology. Sanitary engineeringTD1-1066Environmental sciencesGE1-350ENEnvironment and Natural Resources Journal, Vol 20, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	spent mushroom substrate lignocellulolytic enzymes laccase cellulase dye decolorization briquette Environmental technology. Sanitary engineering TD1-1066 Environmental sciences GE1-350
spellingShingle	spent mushroom substrate lignocellulolytic enzymes laccase cellulase dye decolorization briquette Environmental technology. Sanitary engineering TD1-1066 Environmental sciences GE1-350 Anumeha Vats Anuj Sangam Kurade Srikanth Mutnuri Recovery of Lignocellulolytic Enzymes and Valorization of Spent Mushroom Substrate
description	Spent Mushroom Substrate (SMS) comprises sugarcane bagasse, coconut coir, chicken manure, and paddy straw; inoculated with and farmed for Agaricus bisporus. At present, the waste generation at a mushroom cultivation plant in Goa is 40 tons/day (15,000 tons annually). Valorization of this waste has been explored in terms of extracting lignocellulolytic enzymes and briquette production. SMS was screened for the presence of lignocellulolytic enzymes and then was used to make briquettes. The enzymes found in SMS were cellulase and laccase, which were further concentrated via tangential flow filtration (TFF). Enzyme activity for Cellulase increased by four-fold (from 255.34±1.30 U/mL increased to 1022.21±4.84 U/mL) and Laccase increased by three-fold (from 4.83±0.02 U/mL to 13.21±0.05 U/mL). The concentrated enzyme cocktail was used to decolorize congo red dye. After only eight hours of enzymatic treatment at pH 4.8 on congo red, approx. 40-49% decolorization was accomplished. The color removal was due to the presence of the laccase enzyme. After enzyme extraction, all the residual SMS was utilized to generate briquettes with an initial reduction in its moisture content from 50% to 10%. The resulting briquette gave a Gross Calorific Value of 4,143 Kcal/kg with 12.60% ash content. Thus, SMS proves to be a valuable source for recovering enzymes and a cost-effective material for briquette production rather than going into landfills.
format	article
author	Anumeha Vats Anuj Sangam Kurade Srikanth Mutnuri
author_facet	Anumeha Vats Anuj Sangam Kurade Srikanth Mutnuri
author_sort	Anumeha Vats
title	Recovery of Lignocellulolytic Enzymes and Valorization of Spent Mushroom Substrate
title_short	Recovery of Lignocellulolytic Enzymes and Valorization of Spent Mushroom Substrate
title_full	Recovery of Lignocellulolytic Enzymes and Valorization of Spent Mushroom Substrate
title_fullStr	Recovery of Lignocellulolytic Enzymes and Valorization of Spent Mushroom Substrate
title_full_unstemmed	Recovery of Lignocellulolytic Enzymes and Valorization of Spent Mushroom Substrate
title_sort	recovery of lignocellulolytic enzymes and valorization of spent mushroom substrate
publisher	Mahidol University
publishDate	2021
url	https://doaj.org/article/16a2ffa50a704249a40aac9dad96ab21
work_keys_str_mv	AT anumehavats recoveryoflignocellulolyticenzymesandvalorizationofspentmushroomsubstrate AT anujsangamkurade recoveryoflignocellulolyticenzymesandvalorizationofspentmushroomsubstrate AT srikanthmutnuri recoveryoflignocellulolyticenzymesandvalorizationofspentmushroomsubstrate
_version_	1718373371857600512

Recovery of Lignocellulolytic Enzymes and Valorization of Spent Mushroom Substrate

Ejemplares similares