Trans-cellular introduction of HIV-1 protein Nef induces pathogenic response in Caenorhabditis elegans.

<h4>Background</h4>Caenorhabditis elegans has emerged as a very powerful model for studying the host pathogen interactions. Despite the absence of a naturally occurring viral infection for C. elegans, the model is now being exploited experimentally to study the basic aspects of virus-hos...

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Autores principales:	Aamir Nazir, Shreesh Raj Sammi, Pankaj Singh, Raj Kamal Tripathi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16a9a2fc98324cd3a8efabdbeab39998
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spelling	oai:doaj.org-article:16a9a2fc98324cd3a8efabdbeab399982021-11-18T07:01:46ZTrans-cellular introduction of HIV-1 protein Nef induces pathogenic response in Caenorhabditis elegans.1932-620310.1371/journal.pone.0015312https://doaj.org/article/16a9a2fc98324cd3a8efabdbeab399982010-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21179446/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Caenorhabditis elegans has emerged as a very powerful model for studying the host pathogen interactions. Despite the absence of a naturally occurring viral infection for C. elegans, the model is now being exploited experimentally to study the basic aspects of virus-host interplay. The data generated from recent studies suggests that the virus that infects mammalian cells does infect, replicate and accumulate in C. elegans.<h4>Methodology/principal findings</h4>We took advantage of the easy-to-achieve protein introduction in C. elegans and employing the methodology, we administered HIV-1 protein Nef into live worms. Nef is known to be an important protein for exacerbating HIV-1 pathogenesis in host by enhancing viral replication. The deletion of nef from the viral genome has been reported to inhibit its replication in the host, thereby leading to delayed pathogenesis. Our studies, employing Nef introduction into C. elegans, led to creation of an in-vivo model that allowed us to study, whether or not, the protein induces effect in the whole organism. We observed a marked lipodystrophy, effect on neuromuscular function, impaired fertility and reduced longevity in the worms exposed to Nef. The observed effects resemble to those observed in Nef transgenic mice and most interestingly the effects also relate to some of the pathogenic aspects exhibited by human AIDS patients.<h4>Conclusions/significance</h4>Our studies underline the importance of this in vivo model for studying the interactions of Nef with host proteins, which could further be used for identifying possible inhibitors of such interactions.Aamir NazirShreesh Raj SammiPankaj SinghRaj Kamal TripathiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 12, p e15312 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aamir Nazir Shreesh Raj Sammi Pankaj Singh Raj Kamal Tripathi Trans-cellular introduction of HIV-1 protein Nef induces pathogenic response in Caenorhabditis elegans.
description	<h4>Background</h4>Caenorhabditis elegans has emerged as a very powerful model for studying the host pathogen interactions. Despite the absence of a naturally occurring viral infection for C. elegans, the model is now being exploited experimentally to study the basic aspects of virus-host interplay. The data generated from recent studies suggests that the virus that infects mammalian cells does infect, replicate and accumulate in C. elegans.<h4>Methodology/principal findings</h4>We took advantage of the easy-to-achieve protein introduction in C. elegans and employing the methodology, we administered HIV-1 protein Nef into live worms. Nef is known to be an important protein for exacerbating HIV-1 pathogenesis in host by enhancing viral replication. The deletion of nef from the viral genome has been reported to inhibit its replication in the host, thereby leading to delayed pathogenesis. Our studies, employing Nef introduction into C. elegans, led to creation of an in-vivo model that allowed us to study, whether or not, the protein induces effect in the whole organism. We observed a marked lipodystrophy, effect on neuromuscular function, impaired fertility and reduced longevity in the worms exposed to Nef. The observed effects resemble to those observed in Nef transgenic mice and most interestingly the effects also relate to some of the pathogenic aspects exhibited by human AIDS patients.<h4>Conclusions/significance</h4>Our studies underline the importance of this in vivo model for studying the interactions of Nef with host proteins, which could further be used for identifying possible inhibitors of such interactions.
format	article
author	Aamir Nazir Shreesh Raj Sammi Pankaj Singh Raj Kamal Tripathi
author_facet	Aamir Nazir Shreesh Raj Sammi Pankaj Singh Raj Kamal Tripathi
author_sort	Aamir Nazir
title	Trans-cellular introduction of HIV-1 protein Nef induces pathogenic response in Caenorhabditis elegans.
title_short	Trans-cellular introduction of HIV-1 protein Nef induces pathogenic response in Caenorhabditis elegans.
title_full	Trans-cellular introduction of HIV-1 protein Nef induces pathogenic response in Caenorhabditis elegans.
title_fullStr	Trans-cellular introduction of HIV-1 protein Nef induces pathogenic response in Caenorhabditis elegans.
title_full_unstemmed	Trans-cellular introduction of HIV-1 protein Nef induces pathogenic response in Caenorhabditis elegans.
title_sort	trans-cellular introduction of hiv-1 protein nef induces pathogenic response in caenorhabditis elegans.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/16a9a2fc98324cd3a8efabdbeab39998
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Trans-cellular introduction of HIV-1 protein Nef induces pathogenic response in Caenorhabditis elegans.

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