Metabolomic similarities between bronchoalveolar lavage fluid and plasma in humans and mice

Abstract This observational study catalogues the overlap in metabolites between matched bronchoalveolar lavage fluid (BALF) and plasma, identifies the degree of congruence between these metabolomes in human and mouse, and determines how molecules may change in response to cigarette smoke (CS) exposu...

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Autores principales:	Charmion Cruickshank-Quinn, Roger Powell, Sean Jacobson, Katerina Kechris, Russell P. Bowler, Irina Petrache, Nichole Reisdorph
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16b1e6216ee24f1cab03bfed9b0e6a91
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spelling	oai:doaj.org-article:16b1e6216ee24f1cab03bfed9b0e6a912021-12-02T16:07:44ZMetabolomic similarities between bronchoalveolar lavage fluid and plasma in humans and mice10.1038/s41598-017-05374-12045-2322https://doaj.org/article/16b1e6216ee24f1cab03bfed9b0e6a912017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05374-1https://doaj.org/toc/2045-2322Abstract This observational study catalogues the overlap in metabolites between matched bronchoalveolar lavage fluid (BALF) and plasma, identifies the degree of congruence between these metabolomes in human and mouse, and determines how molecules may change in response to cigarette smoke (CS) exposure. Matched BALF and plasma was collected from mice (ambient air or CS-exposed) and humans (current or former smokers), and analyzed using mass spectrometry. There were 1155 compounds in common in all 4 sample types; fatty acyls and glycerophospholipids strongly overlapped between groups. In humans and mice, more than half of the metabolites present in BALF were also present in plasma. Mouse BALF and human BALF had a strong positive correlation with 2040 metabolites in common, suggesting that mouse models can be used to interrogate human lung metabolome changes. While power was affected by small sample size in the mouse study, the BALF metabolome appeared to be more affected by CS than plasma. CS-exposed mice showed increased plasma and BALF glycerolipids and glycerophospholipids. This is the first report cataloguing the metabolites present across mouse and human, BALF and plasma. Findings are relevant to translational studies where mouse models are used to examine human disease, and where plasma may be interrogated in lieu of BALF or lung tissue.Charmion Cruickshank-QuinnRoger PowellSean JacobsonKaterina KechrisRussell P. BowlerIrina PetracheNichole ReisdorphNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charmion Cruickshank-Quinn Roger Powell Sean Jacobson Katerina Kechris Russell P. Bowler Irina Petrache Nichole Reisdorph Metabolomic similarities between bronchoalveolar lavage fluid and plasma in humans and mice
description	Abstract This observational study catalogues the overlap in metabolites between matched bronchoalveolar lavage fluid (BALF) and plasma, identifies the degree of congruence between these metabolomes in human and mouse, and determines how molecules may change in response to cigarette smoke (CS) exposure. Matched BALF and plasma was collected from mice (ambient air or CS-exposed) and humans (current or former smokers), and analyzed using mass spectrometry. There were 1155 compounds in common in all 4 sample types; fatty acyls and glycerophospholipids strongly overlapped between groups. In humans and mice, more than half of the metabolites present in BALF were also present in plasma. Mouse BALF and human BALF had a strong positive correlation with 2040 metabolites in common, suggesting that mouse models can be used to interrogate human lung metabolome changes. While power was affected by small sample size in the mouse study, the BALF metabolome appeared to be more affected by CS than plasma. CS-exposed mice showed increased plasma and BALF glycerolipids and glycerophospholipids. This is the first report cataloguing the metabolites present across mouse and human, BALF and plasma. Findings are relevant to translational studies where mouse models are used to examine human disease, and where plasma may be interrogated in lieu of BALF or lung tissue.
format	article
author	Charmion Cruickshank-Quinn Roger Powell Sean Jacobson Katerina Kechris Russell P. Bowler Irina Petrache Nichole Reisdorph
author_facet	Charmion Cruickshank-Quinn Roger Powell Sean Jacobson Katerina Kechris Russell P. Bowler Irina Petrache Nichole Reisdorph
author_sort	Charmion Cruickshank-Quinn
title	Metabolomic similarities between bronchoalveolar lavage fluid and plasma in humans and mice
title_short	Metabolomic similarities between bronchoalveolar lavage fluid and plasma in humans and mice
title_full	Metabolomic similarities between bronchoalveolar lavage fluid and plasma in humans and mice
title_fullStr	Metabolomic similarities between bronchoalveolar lavage fluid and plasma in humans and mice
title_full_unstemmed	Metabolomic similarities between bronchoalveolar lavage fluid and plasma in humans and mice
title_sort	metabolomic similarities between bronchoalveolar lavage fluid and plasma in humans and mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/16b1e6216ee24f1cab03bfed9b0e6a91
work_keys_str_mv	AT charmioncruickshankquinn metabolomicsimilaritiesbetweenbronchoalveolarlavagefluidandplasmainhumansandmice AT rogerpowell metabolomicsimilaritiesbetweenbronchoalveolarlavagefluidandplasmainhumansandmice AT seanjacobson metabolomicsimilaritiesbetweenbronchoalveolarlavagefluidandplasmainhumansandmice AT katerinakechris metabolomicsimilaritiesbetweenbronchoalveolarlavagefluidandplasmainhumansandmice AT russellpbowler metabolomicsimilaritiesbetweenbronchoalveolarlavagefluidandplasmainhumansandmice AT irinapetrache metabolomicsimilaritiesbetweenbronchoalveolarlavagefluidandplasmainhumansandmice AT nicholereisdorph metabolomicsimilaritiesbetweenbronchoalveolarlavagefluidandplasmainhumansandmice
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Metabolomic similarities between bronchoalveolar lavage fluid and plasma in humans and mice

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