Isolation and Identification of Antibacterial and Antifungal Compounds from Praxelis clematidea R. M. King & H. Robinson as an Effective Potential Treatment against Rice Pathogens

Bacterial leaf blight and blast diseases caused by the bacterium Xanthomonas oryzae pv. oryzae and the fungus Pyricularia oryzae, respectively, are among the most important infectious diseases affecting rice. We evaluated the antimicrobial eff...

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Autores principales:	Cuong C. Nguyen, Thanh Q. C. Nguyen, Kenji Kanaori, Tran Duy Binh, Le Van Vang, Kaeko Kamei
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Xanthomonas oryzae</i> pv. <i>oryzae</i> <i>Pyricularia oryzae</i> plant extracts <i>Praxelis clematidea</i> natural antimicrobials alternative antimicrobials Agriculture S
Acceso en línea:	https://doaj.org/article/16b2aa1a7a844c959555bde68f3d03ee
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spelling	oai:doaj.org-article:16b2aa1a7a844c959555bde68f3d03ee2021-11-25T16:12:43ZIsolation and Identification of Antibacterial and Antifungal Compounds from <i>Praxelis clematidea</i> R. M. King & H. Robinson as an Effective Potential Treatment against Rice Pathogens10.3390/agronomy111123662073-4395https://doaj.org/article/16b2aa1a7a844c959555bde68f3d03ee2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2366https://doaj.org/toc/2073-4395Bacterial leaf blight and blast diseases caused by the bacterium <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> and the fungus <i>Pyricularia oryzae</i>, respectively, are among the most important infectious diseases affecting rice. We evaluated the antimicrobial effects of compounds derived from <i>Praxelis clematidea</i> on <i>Xanthomonas oryzae</i> and <i>Pyricularia oryzae</i>. The dried aerial parts of <i>Praxelis clematidea</i> were subjected to ethanol extraction, separated by solvent partitioning using hexane, chloroform, ethyl acetate, and water. In vitro assays demonstrated that the main antibacterial and antifungal activities were distributed in the ethyl acetate and chloroform fractions, respectively. These fractions were further separated using silica gel chromatography and reversed-phase chromatography. Finally, we isolated five compounds, <b>1</b>–<b>5</b>, that inhibited the growth of <i>Xanthomonas oryzae</i> in vitro and four compounds, <b>6</b>–<b>9</b>, that exhibited in vitro antifungal activity against <i>Pyricularia oryzae</i>. We evaluated their antimicrobial activities and identified their chemical structures by NMR and mass spectrometry analyses. This is the first study to isolate compound <b>2</b> (4,4′,4″-nitrilotriphenol) as an alternative microbial from natural resources and evaluate its physiological activity. Moreover, this is the first report to demonstrate antibacterial activity in comparison with flavonoids. <i>Praxelis clematidea</i> extracts plausibly exert both antibacterial and antifungal effects, which should be further validated in field trials.Cuong C. NguyenThanh Q. C. NguyenKenji KanaoriTran Duy BinhLe Van VangKaeko KameiMDPI AGarticle<i>Xanthomonas oryzae</i> pv. <i>oryzae</i><i>Pyricularia oryzae</i>plant extracts<i>Praxelis clematidea</i>natural antimicrobialsalternative antimicrobialsAgricultureSENAgronomy, Vol 11, Iss 2366, p 2366 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Xanthomonas oryzae</i> pv. <i>oryzae</i> <i>Pyricularia oryzae</i> plant extracts <i>Praxelis clematidea</i> natural antimicrobials alternative antimicrobials Agriculture S
spellingShingle	<i>Xanthomonas oryzae</i> pv. <i>oryzae</i> <i>Pyricularia oryzae</i> plant extracts <i>Praxelis clematidea</i> natural antimicrobials alternative antimicrobials Agriculture S Cuong C. Nguyen Thanh Q. C. Nguyen Kenji Kanaori Tran Duy Binh Le Van Vang Kaeko Kamei Isolation and Identification of Antibacterial and Antifungal Compounds from <i>Praxelis clematidea</i> R. M. King & H. Robinson as an Effective Potential Treatment against Rice Pathogens
description	Bacterial leaf blight and blast diseases caused by the bacterium <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> and the fungus <i>Pyricularia oryzae</i>, respectively, are among the most important infectious diseases affecting rice. We evaluated the antimicrobial effects of compounds derived from <i>Praxelis clematidea</i> on <i>Xanthomonas oryzae</i> and <i>Pyricularia oryzae</i>. The dried aerial parts of <i>Praxelis clematidea</i> were subjected to ethanol extraction, separated by solvent partitioning using hexane, chloroform, ethyl acetate, and water. In vitro assays demonstrated that the main antibacterial and antifungal activities were distributed in the ethyl acetate and chloroform fractions, respectively. These fractions were further separated using silica gel chromatography and reversed-phase chromatography. Finally, we isolated five compounds, <b>1</b>–<b>5</b>, that inhibited the growth of <i>Xanthomonas oryzae</i> in vitro and four compounds, <b>6</b>–<b>9</b>, that exhibited in vitro antifungal activity against <i>Pyricularia oryzae</i>. We evaluated their antimicrobial activities and identified their chemical structures by NMR and mass spectrometry analyses. This is the first study to isolate compound <b>2</b> (4,4′,4″-nitrilotriphenol) as an alternative microbial from natural resources and evaluate its physiological activity. Moreover, this is the first report to demonstrate antibacterial activity in comparison with flavonoids. <i>Praxelis clematidea</i> extracts plausibly exert both antibacterial and antifungal effects, which should be further validated in field trials.
format	article
author	Cuong C. Nguyen Thanh Q. C. Nguyen Kenji Kanaori Tran Duy Binh Le Van Vang Kaeko Kamei
author_facet	Cuong C. Nguyen Thanh Q. C. Nguyen Kenji Kanaori Tran Duy Binh Le Van Vang Kaeko Kamei
author_sort	Cuong C. Nguyen
title	Isolation and Identification of Antibacterial and Antifungal Compounds from <i>Praxelis clematidea</i> R. M. King & H. Robinson as an Effective Potential Treatment against Rice Pathogens
title_short	Isolation and Identification of Antibacterial and Antifungal Compounds from <i>Praxelis clematidea</i> R. M. King & H. Robinson as an Effective Potential Treatment against Rice Pathogens
title_full	Isolation and Identification of Antibacterial and Antifungal Compounds from <i>Praxelis clematidea</i> R. M. King & H. Robinson as an Effective Potential Treatment against Rice Pathogens
title_fullStr	Isolation and Identification of Antibacterial and Antifungal Compounds from <i>Praxelis clematidea</i> R. M. King & H. Robinson as an Effective Potential Treatment against Rice Pathogens
title_full_unstemmed	Isolation and Identification of Antibacterial and Antifungal Compounds from <i>Praxelis clematidea</i> R. M. King & H. Robinson as an Effective Potential Treatment against Rice Pathogens
title_sort	isolation and identification of antibacterial and antifungal compounds from <i>praxelis clematidea</i> r. m. king & h. robinson as an effective potential treatment against rice pathogens
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/16b2aa1a7a844c959555bde68f3d03ee
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Isolation and Identification of Antibacterial and Antifungal Compounds from <i>Praxelis clematidea</i> R. M. King & H. Robinson as an Effective Potential Treatment against Rice Pathogens

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