lncRNA GAS5/miR-452-5p Reduces Oxidative Stress and Pyroptosis of High-Glucose-Stimulated Renal Tubular Cells
Cuisong Xie,1 Weiling Wu,1 Ainan Tang,2 Ning Luo,1 Yanfei Tan1 1Department of Endocrinology, Chenzhou No.1 People’s Hospital, Chenzhou, Hunan 423000, People’s Republic of China; 2Department of Endocrinology, Chenzhou 3rd People’s Hospital, Chenzhou, Hunan 423000, People...
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oai:doaj.org-article:16b4233aa60c4233bdeaea52c60bba6e2021-12-02T03:25:48ZlncRNA GAS5/miR-452-5p Reduces Oxidative Stress and Pyroptosis of High-Glucose-Stimulated Renal Tubular Cells1178-7007https://doaj.org/article/16b4233aa60c4233bdeaea52c60bba6e2019-12-01T00:00:00Zhttps://www.dovepress.com/lncrna-gas5mir-452-5p-reduces-oxidative-stress-and-pyroptosis-of-high--peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Cuisong Xie,1 Weiling Wu,1 Ainan Tang,2 Ning Luo,1 Yanfei Tan1 1Department of Endocrinology, Chenzhou No.1 People’s Hospital, Chenzhou, Hunan 423000, People’s Republic of China; 2Department of Endocrinology, Chenzhou 3rd People’s Hospital, Chenzhou, Hunan 423000, People’s Republic of ChinaCorrespondence: Cuisong XieDepartment of Endocrinology, Chenzhou No.1 People’s Hospital, 102 Xijie Street, Beihu District, Chenzhou, Hunan 423000, People’s Republic of ChinaEmail xcszgr@163.comBackground: Diabetic nephropathy (DN) is the leading cause of end-stage renal failure worldwide. lncRNAs are demonstrated to improve the DN by changing the expression of miRNAs. This study was aimed to investigate the effect of lncRNA GAS5/miR-452-5p on the inflammation, oxidative stress and pyroptosis of high-glucose-induced renal tubular cells.Methods: HK-2 cells were induced by HG to simulate DN cells. RT-qPCR analysis confirmed the transfection effects and detected the expression of GAS5, NLRP3, caspase1, IL-1β, pro-caspase1, pro-IL-1β, GSDMD-N and miR-452-5p. Western blot analysis determined the protein expression of NLRP3, caspase1, IL-1β, pro-caspase1, pro-IL-1β and GSDMD-N. The expression of GSDMD-N was also verified by immunofluorescence. The levels of TNF-α, IL-6, MCP-1, ROS, MDA and SOD were measured by commercial assay kits, respectively. Dual-luciferase reporter assay indicated that GAS5 could combine with miR-452-5p.Results: GAS5 expression was decreased in HG-induced HK-2 cells. GAS5 overexpression could decrease the levels of TNF-α, IL-6, MCP-1, ROS and MDA and increase the levels of SOD. Moreover, GAS5 overexpression suppressed the expression of NLRP3, caspase1, IL-1β and GSDMD-N, and the results of immunofluorescence verified the above results. miR-452-5p interference could cause the same changes as GAS5 overexpression for HG-induced HK-2 cells, and GAS5 inhibition could reverse the effect of miR-452-5p interference.Conclusion: GAS5 overexpression inhibited the inflammation, oxidative stress and pyroptosis of HG-induced renal tubular cells by downregulating the expression of miR-452-5p.Keywords: lncRNA GAS5, miR-452-5p, oxidative stress, pyroptosis, high glucose, renal tubular cellsXie CWu WTang ALuo NTan YDove Medical Pressarticlelncrna gas5mir-452-5poxidative stresspyroptosishigh glucoserenal tubular cellsSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 2609-2617 (2019) |
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lncrna gas5 mir-452-5p oxidative stress pyroptosis high glucose renal tubular cells Specialties of internal medicine RC581-951 |
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lncrna gas5 mir-452-5p oxidative stress pyroptosis high glucose renal tubular cells Specialties of internal medicine RC581-951 Xie C Wu W Tang A Luo N Tan Y lncRNA GAS5/miR-452-5p Reduces Oxidative Stress and Pyroptosis of High-Glucose-Stimulated Renal Tubular Cells |
| description |
Cuisong Xie,1 Weiling Wu,1 Ainan Tang,2 Ning Luo,1 Yanfei Tan1 1Department of Endocrinology, Chenzhou No.1 People’s Hospital, Chenzhou, Hunan 423000, People’s Republic of China; 2Department of Endocrinology, Chenzhou 3rd People’s Hospital, Chenzhou, Hunan 423000, People’s Republic of ChinaCorrespondence: Cuisong XieDepartment of Endocrinology, Chenzhou No.1 People’s Hospital, 102 Xijie Street, Beihu District, Chenzhou, Hunan 423000, People’s Republic of ChinaEmail xcszgr@163.comBackground: Diabetic nephropathy (DN) is the leading cause of end-stage renal failure worldwide. lncRNAs are demonstrated to improve the DN by changing the expression of miRNAs. This study was aimed to investigate the effect of lncRNA GAS5/miR-452-5p on the inflammation, oxidative stress and pyroptosis of high-glucose-induced renal tubular cells.Methods: HK-2 cells were induced by HG to simulate DN cells. RT-qPCR analysis confirmed the transfection effects and detected the expression of GAS5, NLRP3, caspase1, IL-1β, pro-caspase1, pro-IL-1β, GSDMD-N and miR-452-5p. Western blot analysis determined the protein expression of NLRP3, caspase1, IL-1β, pro-caspase1, pro-IL-1β and GSDMD-N. The expression of GSDMD-N was also verified by immunofluorescence. The levels of TNF-α, IL-6, MCP-1, ROS, MDA and SOD were measured by commercial assay kits, respectively. Dual-luciferase reporter assay indicated that GAS5 could combine with miR-452-5p.Results: GAS5 expression was decreased in HG-induced HK-2 cells. GAS5 overexpression could decrease the levels of TNF-α, IL-6, MCP-1, ROS and MDA and increase the levels of SOD. Moreover, GAS5 overexpression suppressed the expression of NLRP3, caspase1, IL-1β and GSDMD-N, and the results of immunofluorescence verified the above results. miR-452-5p interference could cause the same changes as GAS5 overexpression for HG-induced HK-2 cells, and GAS5 inhibition could reverse the effect of miR-452-5p interference.Conclusion: GAS5 overexpression inhibited the inflammation, oxidative stress and pyroptosis of HG-induced renal tubular cells by downregulating the expression of miR-452-5p.Keywords: lncRNA GAS5, miR-452-5p, oxidative stress, pyroptosis, high glucose, renal tubular cells |
| format |
article |
| author |
Xie C Wu W Tang A Luo N Tan Y |
| author_facet |
Xie C Wu W Tang A Luo N Tan Y |
| author_sort |
Xie C |
| title |
lncRNA GAS5/miR-452-5p Reduces Oxidative Stress and Pyroptosis of High-Glucose-Stimulated Renal Tubular Cells |
| title_short |
lncRNA GAS5/miR-452-5p Reduces Oxidative Stress and Pyroptosis of High-Glucose-Stimulated Renal Tubular Cells |
| title_full |
lncRNA GAS5/miR-452-5p Reduces Oxidative Stress and Pyroptosis of High-Glucose-Stimulated Renal Tubular Cells |
| title_fullStr |
lncRNA GAS5/miR-452-5p Reduces Oxidative Stress and Pyroptosis of High-Glucose-Stimulated Renal Tubular Cells |
| title_full_unstemmed |
lncRNA GAS5/miR-452-5p Reduces Oxidative Stress and Pyroptosis of High-Glucose-Stimulated Renal Tubular Cells |
| title_sort |
lncrna gas5/mir-452-5p reduces oxidative stress and pyroptosis of high-glucose-stimulated renal tubular cells |
| publisher |
Dove Medical Press |
| publishDate |
2019 |
| url |
https://doaj.org/article/16b4233aa60c4233bdeaea52c60bba6e |
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