ZipA and FtsA* stabilize FtsZ-GDP miniring structures

Abstract The cytokinetic division ring of Escherichia coli comprises filaments of FtsZ tethered to the membrane by FtsA and ZipA. Previous results suggested that ZipA is a Z-ring stabilizer, since in vitro experiments it is shown that ZipA enhanced FtsZ assembly and caused the filaments to bundles....

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Autores principales:	Yaodong Chen, Haiyan Huang, Masaki Osawa, Harold P. Erickson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16b42d13f92846d8898a352de1dd6d64
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spelling	oai:doaj.org-article:16b42d13f92846d8898a352de1dd6d642021-12-02T16:06:03ZZipA and FtsA* stabilize FtsZ-GDP miniring structures10.1038/s41598-017-03983-42045-2322https://doaj.org/article/16b42d13f92846d8898a352de1dd6d642017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03983-4https://doaj.org/toc/2045-2322Abstract The cytokinetic division ring of Escherichia coli comprises filaments of FtsZ tethered to the membrane by FtsA and ZipA. Previous results suggested that ZipA is a Z-ring stabilizer, since in vitro experiments it is shown that ZipA enhanced FtsZ assembly and caused the filaments to bundles. However, this function of ZipA has been challenged by recent studies. First, ZipA-induced FtsZ bundling was not significant at pH greater than 7. Second, some FtsA mutants, such as FtsA* were able to bypass the need of ZipA. We reinvestigated the interaction of FtsZ with ZipA in vitro. We found that ZipA not only stabilized and bundled straight filaments of FtsZ-GTP, but also stabilized the highly curved filaments and miniring structures formed by FtsZ-GDP. FtsA* had a similar stabilization of FtsZ-GDP minirings. Our results suggest that ZipA and FtsA* may contribute to constriction by stabilizing this miniring conformation.Yaodong ChenHaiyan HuangMasaki OsawaHarold P. EricksonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yaodong Chen Haiyan Huang Masaki Osawa Harold P. Erickson ZipA and FtsA* stabilize FtsZ-GDP miniring structures
description	Abstract The cytokinetic division ring of Escherichia coli comprises filaments of FtsZ tethered to the membrane by FtsA and ZipA. Previous results suggested that ZipA is a Z-ring stabilizer, since in vitro experiments it is shown that ZipA enhanced FtsZ assembly and caused the filaments to bundles. However, this function of ZipA has been challenged by recent studies. First, ZipA-induced FtsZ bundling was not significant at pH greater than 7. Second, some FtsA mutants, such as FtsA* were able to bypass the need of ZipA. We reinvestigated the interaction of FtsZ with ZipA in vitro. We found that ZipA not only stabilized and bundled straight filaments of FtsZ-GTP, but also stabilized the highly curved filaments and miniring structures formed by FtsZ-GDP. FtsA* had a similar stabilization of FtsZ-GDP minirings. Our results suggest that ZipA and FtsA* may contribute to constriction by stabilizing this miniring conformation.
format	article
author	Yaodong Chen Haiyan Huang Masaki Osawa Harold P. Erickson
author_facet	Yaodong Chen Haiyan Huang Masaki Osawa Harold P. Erickson
author_sort	Yaodong Chen
title	ZipA and FtsA* stabilize FtsZ-GDP miniring structures
title_short	ZipA and FtsA* stabilize FtsZ-GDP miniring structures
title_full	ZipA and FtsA* stabilize FtsZ-GDP miniring structures
title_fullStr	ZipA and FtsA* stabilize FtsZ-GDP miniring structures
title_full_unstemmed	ZipA and FtsA* stabilize FtsZ-GDP miniring structures
title_sort	zipa and ftsa* stabilize ftsz-gdp miniring structures
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/16b42d13f92846d8898a352de1dd6d64
work_keys_str_mv	AT yaodongchen zipaandftsastabilizeftszgdpminiringstructures AT haiyanhuang zipaandftsastabilizeftszgdpminiringstructures AT masakiosawa zipaandftsastabilizeftszgdpminiringstructures AT haroldperickson zipaandftsastabilizeftszgdpminiringstructures
_version_	1718385131858690048

ZipA and FtsA* stabilize FtsZ-GDP miniring structures

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