Knockdown of hsa_circ_0000729 Inhibits the Tumorigenesis of Non-Small Cell Lung Cancer Through Mediation of miR-1281/FOXO3 Axis

Xiao Xie, Fangbao Ding, Haibo Xiao Department of Cardiothoracic Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, People’s Republic of ChinaCorrespondence: Fangbao Ding; Haibo XiaoDepartment of Cardiothoracic Surgery, Xinhua Hospital Affiliate...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xie X, Ding F, Xiao H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	hsa_circ_0000729 mir-1281 foxo3 pyroptosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/16b8e75583db464687ed2a1db4a10150
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:16b8e75583db464687ed2a1db4a10150
record_format	dspace
spelling	oai:doaj.org-article:16b8e75583db464687ed2a1db4a101502021-11-09T18:40:35ZKnockdown of hsa_circ_0000729 Inhibits the Tumorigenesis of Non-Small Cell Lung Cancer Through Mediation of miR-1281/FOXO3 Axis1179-1322https://doaj.org/article/16b8e75583db464687ed2a1db4a101502021-11-01T00:00:00Zhttps://www.dovepress.com/knockdown-of-hsacirc0000729-inhibits-the-tumorigenesis-of-non-small-ce-peer-reviewed-fulltext-article-CMARhttps://doaj.org/toc/1179-1322Xiao Xie, Fangbao Ding, Haibo Xiao Department of Cardiothoracic Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, People’s Republic of ChinaCorrespondence: Fangbao Ding; Haibo XiaoDepartment of Cardiothoracic Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No. 1665 Kongjiang Road, Shanghai, 200092, People’s Republic of ChinaEmail dingfangbao@xinhuamed.com.cn; xiaohaibo@xinhuamed.com.cnBackground: Non-small cell lung cancer (NSCLC) is a subtype of lung cancer which seriously threatens the health of people. Circular RNAs (CircRNAs) are endogenous RNAs which have stable closed structure; they are known to be involved in tumorigenesis of NSCLC. Meanwhile, hsa_circ_0000729 was reported to be upregulated in NSCLC. Nevertheless, the function of hsa_circ_0000729 in NSCLC remains unclear.Methods: Western blot and RT-qPCR were performed to investigate protein and mRNA levels, respectively. CCK-8 assay was performed to test the cell viability and cell death was investigated by flow cytometry. NSCLC cell pyroptosis was observed by electron microscope. In addition, the migration and invasion of NSCLC cells were detected by wound healing and transwell assay. The relation among hsa_circ_0000729, miR-1281 and FOXO3 was explored by dual luciferase reporter assay and RNA pull-down.Results: Hsa_circ_0000729 was found to be upregulated in NSCLC cells, and hsa_circ_0000729 knockdown obviously suppressed the proliferation of NSCLC cells through inducing pyroptosis. In addition, silencing of hsa_circ_0000729 notably inhibited the invasion and migration of NSCLC cells. Meanwhile, hsa_circ_0000729 could bind with miR-1281, and FOXO3 was directly targeted by miR-1281. Moreover, the anti-tumor effect of hsa_circ_0000729 siRNAs on NSCLC was markedly reversed by miR-1281 antagomir. Furthermore, silencing of hsa_circ_0000729 inhibited the tumor growth of NSCLC in vivo.Conclusion: Knockdown of hsa_circ_0000729 inhibits the tumorigenesis of NSCLC through mediation of miR-1281/FOXO3 axis. Thus, hsa_circ_0000729 might be served as a crucial mediator in NSCLC.Keywords: hsa_circ_0000729, miR-1281, FOXO3, pyroptosisXie XDing FXiao HDove Medical Pressarticlehsa_circ_0000729mir-1281foxo3pyroptosisNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancer Management and Research, Vol Volume 13, Pp 8445-8455 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hsa_circ_0000729 mir-1281 foxo3 pyroptosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	hsa_circ_0000729 mir-1281 foxo3 pyroptosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Xie X Ding F Xiao H Knockdown of hsa_circ_0000729 Inhibits the Tumorigenesis of Non-Small Cell Lung Cancer Through Mediation of miR-1281/FOXO3 Axis
description	Xiao Xie, Fangbao Ding, Haibo Xiao Department of Cardiothoracic Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, People’s Republic of ChinaCorrespondence: Fangbao Ding; Haibo XiaoDepartment of Cardiothoracic Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No. 1665 Kongjiang Road, Shanghai, 200092, People’s Republic of ChinaEmail dingfangbao@xinhuamed.com.cn; xiaohaibo@xinhuamed.com.cnBackground: Non-small cell lung cancer (NSCLC) is a subtype of lung cancer which seriously threatens the health of people. Circular RNAs (CircRNAs) are endogenous RNAs which have stable closed structure; they are known to be involved in tumorigenesis of NSCLC. Meanwhile, hsa_circ_0000729 was reported to be upregulated in NSCLC. Nevertheless, the function of hsa_circ_0000729 in NSCLC remains unclear.Methods: Western blot and RT-qPCR were performed to investigate protein and mRNA levels, respectively. CCK-8 assay was performed to test the cell viability and cell death was investigated by flow cytometry. NSCLC cell pyroptosis was observed by electron microscope. In addition, the migration and invasion of NSCLC cells were detected by wound healing and transwell assay. The relation among hsa_circ_0000729, miR-1281 and FOXO3 was explored by dual luciferase reporter assay and RNA pull-down.Results: Hsa_circ_0000729 was found to be upregulated in NSCLC cells, and hsa_circ_0000729 knockdown obviously suppressed the proliferation of NSCLC cells through inducing pyroptosis. In addition, silencing of hsa_circ_0000729 notably inhibited the invasion and migration of NSCLC cells. Meanwhile, hsa_circ_0000729 could bind with miR-1281, and FOXO3 was directly targeted by miR-1281. Moreover, the anti-tumor effect of hsa_circ_0000729 siRNAs on NSCLC was markedly reversed by miR-1281 antagomir. Furthermore, silencing of hsa_circ_0000729 inhibited the tumor growth of NSCLC in vivo.Conclusion: Knockdown of hsa_circ_0000729 inhibits the tumorigenesis of NSCLC through mediation of miR-1281/FOXO3 axis. Thus, hsa_circ_0000729 might be served as a crucial mediator in NSCLC.Keywords: hsa_circ_0000729, miR-1281, FOXO3, pyroptosis
format	article
author	Xie X Ding F Xiao H
author_facet	Xie X Ding F Xiao H
author_sort	Xie X
title	Knockdown of hsa_circ_0000729 Inhibits the Tumorigenesis of Non-Small Cell Lung Cancer Through Mediation of miR-1281/FOXO3 Axis
title_short	Knockdown of hsa_circ_0000729 Inhibits the Tumorigenesis of Non-Small Cell Lung Cancer Through Mediation of miR-1281/FOXO3 Axis
title_full	Knockdown of hsa_circ_0000729 Inhibits the Tumorigenesis of Non-Small Cell Lung Cancer Through Mediation of miR-1281/FOXO3 Axis
title_fullStr	Knockdown of hsa_circ_0000729 Inhibits the Tumorigenesis of Non-Small Cell Lung Cancer Through Mediation of miR-1281/FOXO3 Axis
title_full_unstemmed	Knockdown of hsa_circ_0000729 Inhibits the Tumorigenesis of Non-Small Cell Lung Cancer Through Mediation of miR-1281/FOXO3 Axis
title_sort	knockdown of hsa_circ_0000729 inhibits the tumorigenesis of non-small cell lung cancer through mediation of mir-1281/foxo3 axis
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/16b8e75583db464687ed2a1db4a10150
work_keys_str_mv	AT xiex knockdownofhsacirc0000729inhibitsthetumorigenesisofnonsmallcelllungcancerthroughmediationofmir1281foxo3axis AT dingf knockdownofhsacirc0000729inhibitsthetumorigenesisofnonsmallcelllungcancerthroughmediationofmir1281foxo3axis AT xiaoh knockdownofhsacirc0000729inhibitsthetumorigenesisofnonsmallcelllungcancerthroughmediationofmir1281foxo3axis
_version_	1718440814325006336

Knockdown of hsa_circ_0000729 Inhibits the Tumorigenesis of Non-Small Cell Lung Cancer Through Mediation of miR-1281/FOXO3 Axis

Ejemplares similares