Pulmonary toxicities from a 90-day chronic inhalation study with carbon black nanoparticles in rats related to the systemical immune effects

Chen Chu,1,* Lixiao Zhou,1,* Heran Xie,1,* Zijie Pei,2 Mengyue Zhang,1 Mengqi Wu,1 Shaohui Zhang,3 Luqi Wang,4 Chunfang Zhao,5 Lei Shi,6,7 Ning Zhang,1 Yujie Niu,6,7 Yuxin Zheng,8 Rong Zhang1,7,*1Department of Toxicology, Hebei Medical University, Shijiazhuang 050017, People’s Republic of...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Chu C, Zhou L, Xie H, Pei Z, Zhang M, Wu M, Zhang S, Wang L, Zhao C, Shi L, Zhang N, Niu Y, Zheng Y, Zhang R
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2019
Materias:
Acceso en línea:https://doaj.org/article/16c0a84f9836424ea2ff1e95e6dc65b0
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:16c0a84f9836424ea2ff1e95e6dc65b0
record_format dspace
institution DOAJ
collection DOAJ
language EN
topic Nanoscale CB particles
Pulmonary toxicology
Immune toxicity
Chronic toxicity
Inhalation
Medicine (General)
R5-920
spellingShingle Nanoscale CB particles
Pulmonary toxicology
Immune toxicity
Chronic toxicity
Inhalation
Medicine (General)
R5-920
Chu C
Zhou L
Xie H
Pei Z
Zhang M
Wu M
Zhang S
Wang L
Zhao C
Shi L
Zhang N
Niu Y
Zheng Y
Zhang R
Pulmonary toxicities from a 90-day chronic inhalation study with carbon black nanoparticles in rats related to the systemical immune effects
description Chen Chu,1,* Lixiao Zhou,1,* Heran Xie,1,* Zijie Pei,2 Mengyue Zhang,1 Mengqi Wu,1 Shaohui Zhang,3 Luqi Wang,4 Chunfang Zhao,5 Lei Shi,6,7 Ning Zhang,1 Yujie Niu,6,7 Yuxin Zheng,8 Rong Zhang1,7,*1Department of Toxicology, Hebei Medical University, Shijiazhuang 050017, People’s Republic of China; 2Department of Pathology, Medical School, China Three Gorge University, Yichang, 443002, People’s Republic of China; 3Department of Experimental Center, The Third Hospital of Hebei Medical University, Shijiazhuang, 050051, People’s Republic of China; 4Riodiology, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, People’s Republic of China; 5Histology and Embryology, Hebei Medical University, Shijiazhuang 050017, People’s Republic of China; 6Occupational Health and Environmental Health, Hebei Medical University, Shijiazhuang 050017, People’s Republic of China; 7Hebei Key Laboratory of Environment and Human Health, Hebei Medical University, Shijiazhuang, People’s Republic of China; 8Department of Toxicology, Public Health College, Qingdao University, Qingdao, 266000, People’s Republic of China*These authors contributed equally to this workBackground: Recent years, there occurs heavy haze pollution in northern China during wintertime. The potential influence of airborne particulate matter (PM) on human health attracts great concern. The fuel-derived PM in the inhalable size range is dominated by aggregates of nanoparticles of Carbon black (CB). However, there are still lack of evidences especially regarding long-term exposure to explain the chronic effects of nanoscaled CB and the relative mechanism.Purpose: The objective of this study was to identify the potential mechanism of chronic effects of nanoscale CB. The systemic toxicity, immune suppression or activity and local toxicity were evaluated.Methods: 32 rats were divided into 2 groups: 30 mg/m3 CB exposure (nose only, 90 d, 6h/d) and control (clean air). Half of rats were scarified after exposure and another half of rats recovered for 14 days. Eight rats in each group were executed the lung function tests using a ventilated bias flow whole body plethysmograph (WBP). SDS-PAGE protocol was used to detect the deposition and retention of CB in lung of rats. HE staining was used to observe the changes of histopathology. Cell apoptosis was examined by TUNEL assay or flow cytometry. The levels of IL-6, IL-8, IL-17 and TNF-α in serum and lung tissue were evaluated with commercially available ELISA kit. The peripheral blood cell counts were detected by Auto 5-diff hematology analyzer.Results: The lung burden of CB was 16 mg in lung of rats after a 90-day exposure by MPPD. Fourteen percentages of the amount of CB accumulated at the end of the exposure period was cleared from the lung during the 14 dys recovery period. The lung function was significantly decreased and could not recover after a short time recovery. The fibroblasts and granuloma formation were found in lung. The levels of apoptosis and DNA damages were significantly increased in lung cells after CB inhalation. The cytokines levels in lung but not in serum were significantly increased in CB exposure group. The cell counts of WBC, monocytes and neutrophils had 1.72, 3.13, and 2.73-fold increases after CB exposure, respectively. The percentages of CD4+ lymphocytes and the rates of CD4+/CD8+ were statistically increased after CB exposure. The stimulation indexes of the peripheral blood lymphocytes were significantly decreased after CB exposure. In the CB exposure group, the disrupted histomorphology of thymus and spleen were found as well as the early apoptotic thymocytes had a 2.36-fold increase.Conclusion: CB induced the localized or direct toxicity and systemic immune toxicity. The direct and systemic immune responses had a combined effect on the lung damages caused by CB.Keywords: nanoscale CB particles, pulmonary toxicology, immune toxicity, chronic toxicity, inhalation
format article
author Chu C
Zhou L
Xie H
Pei Z
Zhang M
Wu M
Zhang S
Wang L
Zhao C
Shi L
Zhang N
Niu Y
Zheng Y
Zhang R
author_facet Chu C
Zhou L
Xie H
Pei Z
Zhang M
Wu M
Zhang S
Wang L
Zhao C
Shi L
Zhang N
Niu Y
Zheng Y
Zhang R
author_sort Chu C
title Pulmonary toxicities from a 90-day chronic inhalation study with carbon black nanoparticles in rats related to the systemical immune effects
title_short Pulmonary toxicities from a 90-day chronic inhalation study with carbon black nanoparticles in rats related to the systemical immune effects
title_full Pulmonary toxicities from a 90-day chronic inhalation study with carbon black nanoparticles in rats related to the systemical immune effects
title_fullStr Pulmonary toxicities from a 90-day chronic inhalation study with carbon black nanoparticles in rats related to the systemical immune effects
title_full_unstemmed Pulmonary toxicities from a 90-day chronic inhalation study with carbon black nanoparticles in rats related to the systemical immune effects
title_sort pulmonary toxicities from a 90-day chronic inhalation study with carbon black nanoparticles in rats related to the systemical immune effects
publisher Dove Medical Press
publishDate 2019
url https://doaj.org/article/16c0a84f9836424ea2ff1e95e6dc65b0
work_keys_str_mv AT chuc pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT zhoul pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT xieh pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT peiz pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT zhangm pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT wum pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT zhangs pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT wangl pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT zhaoc pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT shil pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT zhangn pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT niuy pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT zhengy pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
AT zhangr pulmonarytoxicitiesfroma90daychronicinhalationstudywithcarbonblacknanoparticlesinratsrelatedtothesystemicalimmuneeffects
_version_ 1718403422236966912
spelling oai:doaj.org-article:16c0a84f9836424ea2ff1e95e6dc65b02021-12-02T00:50:42ZPulmonary toxicities from a 90-day chronic inhalation study with carbon black nanoparticles in rats related to the systemical immune effects1178-2013https://doaj.org/article/16c0a84f9836424ea2ff1e95e6dc65b02019-04-01T00:00:00Zhttps://www.dovepress.com/pulmonary-toxicities-from-a-90-day-chronic-inhalation-study-with-carbo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chen Chu,1,* Lixiao Zhou,1,* Heran Xie,1,* Zijie Pei,2 Mengyue Zhang,1 Mengqi Wu,1 Shaohui Zhang,3 Luqi Wang,4 Chunfang Zhao,5 Lei Shi,6,7 Ning Zhang,1 Yujie Niu,6,7 Yuxin Zheng,8 Rong Zhang1,7,*1Department of Toxicology, Hebei Medical University, Shijiazhuang 050017, People’s Republic of China; 2Department of Pathology, Medical School, China Three Gorge University, Yichang, 443002, People’s Republic of China; 3Department of Experimental Center, The Third Hospital of Hebei Medical University, Shijiazhuang, 050051, People’s Republic of China; 4Riodiology, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, People’s Republic of China; 5Histology and Embryology, Hebei Medical University, Shijiazhuang 050017, People’s Republic of China; 6Occupational Health and Environmental Health, Hebei Medical University, Shijiazhuang 050017, People’s Republic of China; 7Hebei Key Laboratory of Environment and Human Health, Hebei Medical University, Shijiazhuang, People’s Republic of China; 8Department of Toxicology, Public Health College, Qingdao University, Qingdao, 266000, People’s Republic of China*These authors contributed equally to this workBackground: Recent years, there occurs heavy haze pollution in northern China during wintertime. The potential influence of airborne particulate matter (PM) on human health attracts great concern. The fuel-derived PM in the inhalable size range is dominated by aggregates of nanoparticles of Carbon black (CB). However, there are still lack of evidences especially regarding long-term exposure to explain the chronic effects of nanoscaled CB and the relative mechanism.Purpose: The objective of this study was to identify the potential mechanism of chronic effects of nanoscale CB. The systemic toxicity, immune suppression or activity and local toxicity were evaluated.Methods: 32 rats were divided into 2 groups: 30 mg/m3 CB exposure (nose only, 90 d, 6h/d) and control (clean air). Half of rats were scarified after exposure and another half of rats recovered for 14 days. Eight rats in each group were executed the lung function tests using a ventilated bias flow whole body plethysmograph (WBP). SDS-PAGE protocol was used to detect the deposition and retention of CB in lung of rats. HE staining was used to observe the changes of histopathology. Cell apoptosis was examined by TUNEL assay or flow cytometry. The levels of IL-6, IL-8, IL-17 and TNF-α in serum and lung tissue were evaluated with commercially available ELISA kit. The peripheral blood cell counts were detected by Auto 5-diff hematology analyzer.Results: The lung burden of CB was 16 mg in lung of rats after a 90-day exposure by MPPD. Fourteen percentages of the amount of CB accumulated at the end of the exposure period was cleared from the lung during the 14 dys recovery period. The lung function was significantly decreased and could not recover after a short time recovery. The fibroblasts and granuloma formation were found in lung. The levels of apoptosis and DNA damages were significantly increased in lung cells after CB inhalation. The cytokines levels in lung but not in serum were significantly increased in CB exposure group. The cell counts of WBC, monocytes and neutrophils had 1.72, 3.13, and 2.73-fold increases after CB exposure, respectively. The percentages of CD4+ lymphocytes and the rates of CD4+/CD8+ were statistically increased after CB exposure. The stimulation indexes of the peripheral blood lymphocytes were significantly decreased after CB exposure. In the CB exposure group, the disrupted histomorphology of thymus and spleen were found as well as the early apoptotic thymocytes had a 2.36-fold increase.Conclusion: CB induced the localized or direct toxicity and systemic immune toxicity. The direct and systemic immune responses had a combined effect on the lung damages caused by CB.Keywords: nanoscale CB particles, pulmonary toxicology, immune toxicity, chronic toxicity, inhalationChu CZhou LXie HPei ZZhang MWu MZhang SWang LZhao CShi LZhang NNiu YZheng YZhang RDove Medical PressarticleNanoscale CB particlesPulmonary toxicologyImmune toxicityChronic toxicityInhalationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 2995-3013 (2019)