Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia

Irawati W, Ompusunggu NP, Susilowati DN, Yuwono T. 2019. Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia. Biodiversitas 20: 344-349. The use of indigenous bacteria as bioremediation agents in wastewater treatments plan...

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Autores principales:	WAHYU IRAWATI, NOMMENSEN PANGIHUTAN OMPUSUNGGU, DWI NINGSIH SUSILOWATI, TRIWIBOWO YUWONO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	copper accumulation and removal copper-resistant bacteria phylogenetic tree Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/16c4993698284e2c8055fe03a3eddd44
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spelling	oai:doaj.org-article:16c4993698284e2c8055fe03a3eddd442021-11-16T14:11:27ZMolecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia1412-033X2085-472210.13057/biodiv/d200206https://doaj.org/article/16c4993698284e2c8055fe03a3eddd442019-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3446https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Irawati W, Ompusunggu NP, Susilowati DN, Yuwono T. 2019. Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia. Biodiversitas 20: 344-349. The use of indigenous bacteria as bioremediation agents in wastewater treatments plant is a promising solution for tackling the environmental problem. The objectives of this research were to establish the phylogenetic tree and physiological characterization of copper-resistant bacteria isolated from Cikapundung River, West Java, Indonesia. A phylogenetic tree was constructed based on 16S rDNA sequences. Physiological characterization including growth and the potency of bacterial strain for copper accumulation and copper removal was also studied. Five highly copper-resistant bacteria designated as strains CN1, CN2, CN5, CN6, and CN8 have been isolated from Cikapundung river with the MICs (Minimum Inhibitory Concentration) of 5 mM-8mM. Sequence alignment and phylogenetic analysis showed that strains CN1 and CN6 belong to Klebsiella pneumoniae with the similarity of 99%. Meanwhile, strain CN2, CN5, and CN8 belong to Acinetobacter calcoaceticus, Acinetobacter sp. and Escherichia coli with the similarity of 99%, 96%, and 95%, respectively. The highest resistant bacterial isolates were strains CN6 and CN8 with the MICs of 8 mM. The highest accumulation capability was found in strain CN1 with a total of 4.62 mg/g dry weight of cells. Meanwhile, strain CN8 demonstrated the highest copper removal with the value of 48.15%.WAHYU IRAWATINOMMENSEN PANGIHUTAN OMPUSUNGGUDWI NINGSIH SUSILOWATITRIWIBOWO YUWONOMBI & UNS Soloarticlecopper accumulation and removalcopper-resistant bacteriaphylogenetic treeBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 2, Pp 344-349 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	copper accumulation and removal copper-resistant bacteria phylogenetic tree Biology (General) QH301-705.5
spellingShingle	copper accumulation and removal copper-resistant bacteria phylogenetic tree Biology (General) QH301-705.5 WAHYU IRAWATI NOMMENSEN PANGIHUTAN OMPUSUNGGU DWI NINGSIH SUSILOWATI TRIWIBOWO YUWONO Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia
description	Irawati W, Ompusunggu NP, Susilowati DN, Yuwono T. 2019. Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia. Biodiversitas 20: 344-349. The use of indigenous bacteria as bioremediation agents in wastewater treatments plant is a promising solution for tackling the environmental problem. The objectives of this research were to establish the phylogenetic tree and physiological characterization of copper-resistant bacteria isolated from Cikapundung River, West Java, Indonesia. A phylogenetic tree was constructed based on 16S rDNA sequences. Physiological characterization including growth and the potency of bacterial strain for copper accumulation and copper removal was also studied. Five highly copper-resistant bacteria designated as strains CN1, CN2, CN5, CN6, and CN8 have been isolated from Cikapundung river with the MICs (Minimum Inhibitory Concentration) of 5 mM-8mM. Sequence alignment and phylogenetic analysis showed that strains CN1 and CN6 belong to Klebsiella pneumoniae with the similarity of 99%. Meanwhile, strain CN2, CN5, and CN8 belong to Acinetobacter calcoaceticus, Acinetobacter sp. and Escherichia coli with the similarity of 99%, 96%, and 95%, respectively. The highest resistant bacterial isolates were strains CN6 and CN8 with the MICs of 8 mM. The highest accumulation capability was found in strain CN1 with a total of 4.62 mg/g dry weight of cells. Meanwhile, strain CN8 demonstrated the highest copper removal with the value of 48.15%.
format	article
author	WAHYU IRAWATI NOMMENSEN PANGIHUTAN OMPUSUNGGU DWI NINGSIH SUSILOWATI TRIWIBOWO YUWONO
author_facet	WAHYU IRAWATI NOMMENSEN PANGIHUTAN OMPUSUNGGU DWI NINGSIH SUSILOWATI TRIWIBOWO YUWONO
author_sort	WAHYU IRAWATI
title	Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia
title_short	Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia
title_full	Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia
title_fullStr	Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia
title_full_unstemmed	Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia
title_sort	molecular and physiological characterization of indigenous copper-resistant bacteria from cikapundung river, west java, indonesia
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/16c4993698284e2c8055fe03a3eddd44
work_keys_str_mv	AT wahyuirawati molecularandphysiologicalcharacterizationofindigenouscopperresistantbacteriafromcikapundungriverwestjavaindonesia AT nommensenpangihutanompusunggu molecularandphysiologicalcharacterizationofindigenouscopperresistantbacteriafromcikapundungriverwestjavaindonesia AT dwiningsihsusilowati molecularandphysiologicalcharacterizationofindigenouscopperresistantbacteriafromcikapundungriverwestjavaindonesia AT triwibowoyuwono molecularandphysiologicalcharacterizationofindigenouscopperresistantbacteriafromcikapundungriverwestjavaindonesia
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Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia

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