SPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level

Abstract The oral squamous cell carcinoma (OSCC), which has a high morbidity rate, affects patients worldwide. Changes in SPINK7 in precancerous lesions could promote oncogenesis. Our aim was to evaluate SPINK7 as a potential molecular biomarker which predicts OSCC stages, compared to: HER2, TP53, R...

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Autores principales:	Gina Pennacchiotti, Fabio Valdés-Gutiérrez, Wilfredo Alejandro González-Arriagada, Héctor Federico Montes, Judith Maria Roxana Parra, Valeria Andrea Guida, Silvina Esther Gómez, Martin Eduardo Guerrero-Gimenez, Juan Manuel Fernandez-Muñoz, Felipe Carlos Martin Zoppino, Rubén Walter Carón, Marcelo Eduardo Ezquer, Ricardo Fernández-Ramires, Flavia Alejandra Bruna
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16c942f7f2eb4db38e03d730d4b87dae
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spelling	oai:doaj.org-article:16c942f7f2eb4db38e03d730d4b87dae2021-12-02T17:04:07ZSPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level10.1038/s41598-021-86208-z2045-2322https://doaj.org/article/16c942f7f2eb4db38e03d730d4b87dae2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86208-zhttps://doaj.org/toc/2045-2322Abstract The oral squamous cell carcinoma (OSCC), which has a high morbidity rate, affects patients worldwide. Changes in SPINK7 in precancerous lesions could promote oncogenesis. Our aim was to evaluate SPINK7 as a potential molecular biomarker which predicts OSCC stages, compared to: HER2, TP53, RB1, NFKB and CYP4B1. This study used oral biopsies from three patient groups: dysplasia (n = 33), less invasive (n = 28) and highly invasive OSCC (n = 18). The control group consisted of clinically suspicious cases later to be confirmed as normal mucosa (n = 20). Gene levels of SPINK7, P53, RB, NFKB and CYP4B1 were quantified by qPCR. SPINK7 levels were correlated with a cohort of 330 patients from the TCGA. Also, SPINK7, HER2, TP53, and RB1, were evaluated by immunohistofluorescence. One-way Kruskal–Wallis test and Dunn's post-hoc with a p < 0.05 significance was used to analyze data. In OSCC, the SPINK7 expression had down regulated while P53, RB, NFKB and CYP4B1 had up regulated (p < 0.001). SPINK7 had also diminished in TCGA patients (p = 2.10e-6). In less invasive OSCC, SPINK7 and HER2 proteins had decreased while TP53 and RB1 had increased with respect to the other groups (p < 0.05). The changes of SPINK7 accompanied by HER2, P53 and RB1 can be used to classify the molecular stage of OSCC lesions allowing a diagnosis at molecular and histopathological levels.Gina PennacchiottiFabio Valdés-GutiérrezWilfredo Alejandro González-ArriagadaHéctor Federico MontesJudith Maria Roxana ParraValeria Andrea GuidaSilvina Esther GómezMartin Eduardo Guerrero-GimenezJuan Manuel Fernandez-MuñozFelipe Carlos Martin ZoppinoRubén Walter CarónMarcelo Eduardo EzquerRicardo Fernández-RamiresFlavia Alejandra BrunaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gina Pennacchiotti Fabio Valdés-Gutiérrez Wilfredo Alejandro González-Arriagada Héctor Federico Montes Judith Maria Roxana Parra Valeria Andrea Guida Silvina Esther Gómez Martin Eduardo Guerrero-Gimenez Juan Manuel Fernandez-Muñoz Felipe Carlos Martin Zoppino Rubén Walter Carón Marcelo Eduardo Ezquer Ricardo Fernández-Ramires Flavia Alejandra Bruna SPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level
description	Abstract The oral squamous cell carcinoma (OSCC), which has a high morbidity rate, affects patients worldwide. Changes in SPINK7 in precancerous lesions could promote oncogenesis. Our aim was to evaluate SPINK7 as a potential molecular biomarker which predicts OSCC stages, compared to: HER2, TP53, RB1, NFKB and CYP4B1. This study used oral biopsies from three patient groups: dysplasia (n = 33), less invasive (n = 28) and highly invasive OSCC (n = 18). The control group consisted of clinically suspicious cases later to be confirmed as normal mucosa (n = 20). Gene levels of SPINK7, P53, RB, NFKB and CYP4B1 were quantified by qPCR. SPINK7 levels were correlated with a cohort of 330 patients from the TCGA. Also, SPINK7, HER2, TP53, and RB1, were evaluated by immunohistofluorescence. One-way Kruskal–Wallis test and Dunn's post-hoc with a p < 0.05 significance was used to analyze data. In OSCC, the SPINK7 expression had down regulated while P53, RB, NFKB and CYP4B1 had up regulated (p < 0.001). SPINK7 had also diminished in TCGA patients (p = 2.10e-6). In less invasive OSCC, SPINK7 and HER2 proteins had decreased while TP53 and RB1 had increased with respect to the other groups (p < 0.05). The changes of SPINK7 accompanied by HER2, P53 and RB1 can be used to classify the molecular stage of OSCC lesions allowing a diagnosis at molecular and histopathological levels.
format	article
author	Gina Pennacchiotti Fabio Valdés-Gutiérrez Wilfredo Alejandro González-Arriagada Héctor Federico Montes Judith Maria Roxana Parra Valeria Andrea Guida Silvina Esther Gómez Martin Eduardo Guerrero-Gimenez Juan Manuel Fernandez-Muñoz Felipe Carlos Martin Zoppino Rubén Walter Carón Marcelo Eduardo Ezquer Ricardo Fernández-Ramires Flavia Alejandra Bruna
author_facet	Gina Pennacchiotti Fabio Valdés-Gutiérrez Wilfredo Alejandro González-Arriagada Héctor Federico Montes Judith Maria Roxana Parra Valeria Andrea Guida Silvina Esther Gómez Martin Eduardo Guerrero-Gimenez Juan Manuel Fernandez-Muñoz Felipe Carlos Martin Zoppino Rubén Walter Carón Marcelo Eduardo Ezquer Ricardo Fernández-Ramires Flavia Alejandra Bruna
author_sort	Gina Pennacchiotti
title	SPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level
title_short	SPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level
title_full	SPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level
title_fullStr	SPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level
title_full_unstemmed	SPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level
title_sort	spink7 expression changes accompanied by her2, p53 and rb1 can be relevant in predicting oral squamous cell carcinoma at a molecular level
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/16c942f7f2eb4db38e03d730d4b87dae
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SPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level

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