Highly phosphorylated functionalized rice starch produced by transgenic rice expressing the potato GWD1 gene

Abstract Starch phosphorylation occurs naturally during starch metabolism in the plant and is catalysed by glucan water dikinases (GWD1) and phosphoglucan water dikinase/glucan water dikinase 3 (PWD/GWD3). We generated six stable individual transgenic lines by over-expressing the potato GWD1 in rice...

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Autores principales:	Yaling Chen, Xiao Sun, Xin Zhou, Kim H. Hebelstrup, Andreas Blennow, Jinsong Bao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16c9d7edf117427a84811359b56e4363
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spelling	oai:doaj.org-article:16c9d7edf117427a84811359b56e43632021-12-02T11:40:22ZHighly phosphorylated functionalized rice starch produced by transgenic rice expressing the potato GWD1 gene10.1038/s41598-017-03637-52045-2322https://doaj.org/article/16c9d7edf117427a84811359b56e43632017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03637-5https://doaj.org/toc/2045-2322Abstract Starch phosphorylation occurs naturally during starch metabolism in the plant and is catalysed by glucan water dikinases (GWD1) and phosphoglucan water dikinase/glucan water dikinase 3 (PWD/GWD3). We generated six stable individual transgenic lines by over-expressing the potato GWD1 in rice. Transgenic rice grain starch had 9-fold higher 6-phospho (6-P) monoesters and double amounts of 3-phospho (3-P) monoesters, respectively, compared to control grain. The shape and topography of the transgenic starch granules were moderately altered including surface pores and less well defined edges. The gelatinization temperatures of both rice flour and extracted starch were significantly lower than those of the control and hence negatively correlated with the starch phosphate content. The 6-P content was positively correlated with amylose content and relatively long amylopectin chains with DP25-36, and the 3-P content was positively correlated with short chains of DP6-12. The starch pasting temperature, peak viscosity and the breakdown were lower but the setback was higher for transgenic rice flour. The 6-P content was negatively correlated with texture adhesiveness but positively correlated with the cohesiveness of rice flour gels. Our data demonstrate a way forward to employ a starch bioengineering approach for clean modification of starch, opening up completely new applications for rice starch.Yaling ChenXiao SunXin ZhouKim H. HebelstrupAndreas BlennowJinsong BaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yaling Chen Xiao Sun Xin Zhou Kim H. Hebelstrup Andreas Blennow Jinsong Bao Highly phosphorylated functionalized rice starch produced by transgenic rice expressing the potato GWD1 gene
description	Abstract Starch phosphorylation occurs naturally during starch metabolism in the plant and is catalysed by glucan water dikinases (GWD1) and phosphoglucan water dikinase/glucan water dikinase 3 (PWD/GWD3). We generated six stable individual transgenic lines by over-expressing the potato GWD1 in rice. Transgenic rice grain starch had 9-fold higher 6-phospho (6-P) monoesters and double amounts of 3-phospho (3-P) monoesters, respectively, compared to control grain. The shape and topography of the transgenic starch granules were moderately altered including surface pores and less well defined edges. The gelatinization temperatures of both rice flour and extracted starch were significantly lower than those of the control and hence negatively correlated with the starch phosphate content. The 6-P content was positively correlated with amylose content and relatively long amylopectin chains with DP25-36, and the 3-P content was positively correlated with short chains of DP6-12. The starch pasting temperature, peak viscosity and the breakdown were lower but the setback was higher for transgenic rice flour. The 6-P content was negatively correlated with texture adhesiveness but positively correlated with the cohesiveness of rice flour gels. Our data demonstrate a way forward to employ a starch bioengineering approach for clean modification of starch, opening up completely new applications for rice starch.
format	article
author	Yaling Chen Xiao Sun Xin Zhou Kim H. Hebelstrup Andreas Blennow Jinsong Bao
author_facet	Yaling Chen Xiao Sun Xin Zhou Kim H. Hebelstrup Andreas Blennow Jinsong Bao
author_sort	Yaling Chen
title	Highly phosphorylated functionalized rice starch produced by transgenic rice expressing the potato GWD1 gene
title_short	Highly phosphorylated functionalized rice starch produced by transgenic rice expressing the potato GWD1 gene
title_full	Highly phosphorylated functionalized rice starch produced by transgenic rice expressing the potato GWD1 gene
title_fullStr	Highly phosphorylated functionalized rice starch produced by transgenic rice expressing the potato GWD1 gene
title_full_unstemmed	Highly phosphorylated functionalized rice starch produced by transgenic rice expressing the potato GWD1 gene
title_sort	highly phosphorylated functionalized rice starch produced by transgenic rice expressing the potato gwd1 gene
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/16c9d7edf117427a84811359b56e4363
work_keys_str_mv	AT yalingchen highlyphosphorylatedfunctionalizedricestarchproducedbytransgenicriceexpressingthepotatogwd1gene AT xiaosun highlyphosphorylatedfunctionalizedricestarchproducedbytransgenicriceexpressingthepotatogwd1gene AT xinzhou highlyphosphorylatedfunctionalizedricestarchproducedbytransgenicriceexpressingthepotatogwd1gene AT kimhhebelstrup highlyphosphorylatedfunctionalizedricestarchproducedbytransgenicriceexpressingthepotatogwd1gene AT andreasblennow highlyphosphorylatedfunctionalizedricestarchproducedbytransgenicriceexpressingthepotatogwd1gene AT jinsongbao highlyphosphorylatedfunctionalizedricestarchproducedbytransgenicriceexpressingthepotatogwd1gene
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Highly phosphorylated functionalized rice starch produced by transgenic rice expressing the potato GWD1 gene

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