Viral Diversity of House Mice in New York City

ABSTRACT The microbiome of wild Mus musculus (house mouse), a globally distributed invasive pest that resides in close contact with humans in urban centers, is largely unexplored. Here, we report analysis of the fecal virome of house mice in residential buildings in New York City, NY. Mice were coll...

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Autores principales:	Simon H. Williams, Xiaoyu Che, Joel A. Garcia, John D. Klena, Bohyun Lee, Dorothy Muller, Werner Ulrich, Robert M. Corrigan, Stuart Nichol, Komal Jain, W. Ian Lipkin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	environmental microbiology microbial ecology microbial genetics New York City veterinary microbiology mouse virome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/16ce31fd5afd402e9260af65e027c78d
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spelling	oai:doaj.org-article:16ce31fd5afd402e9260af65e027c78d2021-11-15T15:53:27ZViral Diversity of House Mice in New York City10.1128/mBio.01354-172150-7511https://doaj.org/article/16ce31fd5afd402e9260af65e027c78d2018-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01354-17https://doaj.org/toc/2150-7511ABSTRACT The microbiome of wild Mus musculus (house mouse), a globally distributed invasive pest that resides in close contact with humans in urban centers, is largely unexplored. Here, we report analysis of the fecal virome of house mice in residential buildings in New York City, NY. Mice were collected at seven sites in Manhattan, Queens, Brooklyn, and the Bronx over a period of 1 year. Unbiased high-throughput sequencing of feces revealed 36 viruses from 18 families and 21 genera, including at least 6 novel viruses and 3 novel genera. A representative screen of 15 viruses by PCR confirmed the presence of 13 of these viruses in liver. We identified an uneven distribution of diversity, with several viruses being associated with specific locations. Higher mouse weight was associated with an increase in the number of viruses detected per mouse, after adjusting for site, sex, and length. We found neither genetic footprints to known human viral pathogens nor antibodies to lymphocytic choriomeningitis virus. IMPORTANCE Mice carry a wide range of infectious agents with zoonotic potential. Their proximity to humans in the built environment is therefore a concern for public health. Laboratory mice are also the most common experimental model for investigating the pathobiology of infectious diseases. In this survey of mice trapped in multiple locations within New York City over a period of 1 year, we found a diverse collection of viruses that includes some previously not associated with house mice and others that appear to be novel. Although we found no known human pathogens, our findings provide insights into viral ecology and may yield models that have utility for clinical microbiology.Simon H. WilliamsXiaoyu CheJoel A. GarciaJohn D. KlenaBohyun LeeDorothy MullerWerner UlrichRobert M. CorriganStuart NicholKomal JainW. Ian LipkinAmerican Society for Microbiologyarticleenvironmental microbiologymicrobial ecologymicrobial geneticsNew York Cityveterinary microbiologymouse viromeMicrobiologyQR1-502ENmBio, Vol 9, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	environmental microbiology microbial ecology microbial genetics New York City veterinary microbiology mouse virome Microbiology QR1-502
spellingShingle	environmental microbiology microbial ecology microbial genetics New York City veterinary microbiology mouse virome Microbiology QR1-502 Simon H. Williams Xiaoyu Che Joel A. Garcia John D. Klena Bohyun Lee Dorothy Muller Werner Ulrich Robert M. Corrigan Stuart Nichol Komal Jain W. Ian Lipkin Viral Diversity of House Mice in New York City
description	ABSTRACT The microbiome of wild Mus musculus (house mouse), a globally distributed invasive pest that resides in close contact with humans in urban centers, is largely unexplored. Here, we report analysis of the fecal virome of house mice in residential buildings in New York City, NY. Mice were collected at seven sites in Manhattan, Queens, Brooklyn, and the Bronx over a period of 1 year. Unbiased high-throughput sequencing of feces revealed 36 viruses from 18 families and 21 genera, including at least 6 novel viruses and 3 novel genera. A representative screen of 15 viruses by PCR confirmed the presence of 13 of these viruses in liver. We identified an uneven distribution of diversity, with several viruses being associated with specific locations. Higher mouse weight was associated with an increase in the number of viruses detected per mouse, after adjusting for site, sex, and length. We found neither genetic footprints to known human viral pathogens nor antibodies to lymphocytic choriomeningitis virus. IMPORTANCE Mice carry a wide range of infectious agents with zoonotic potential. Their proximity to humans in the built environment is therefore a concern for public health. Laboratory mice are also the most common experimental model for investigating the pathobiology of infectious diseases. In this survey of mice trapped in multiple locations within New York City over a period of 1 year, we found a diverse collection of viruses that includes some previously not associated with house mice and others that appear to be novel. Although we found no known human pathogens, our findings provide insights into viral ecology and may yield models that have utility for clinical microbiology.
format	article
author	Simon H. Williams Xiaoyu Che Joel A. Garcia John D. Klena Bohyun Lee Dorothy Muller Werner Ulrich Robert M. Corrigan Stuart Nichol Komal Jain W. Ian Lipkin
author_facet	Simon H. Williams Xiaoyu Che Joel A. Garcia John D. Klena Bohyun Lee Dorothy Muller Werner Ulrich Robert M. Corrigan Stuart Nichol Komal Jain W. Ian Lipkin
author_sort	Simon H. Williams
title	Viral Diversity of House Mice in New York City
title_short	Viral Diversity of House Mice in New York City
title_full	Viral Diversity of House Mice in New York City
title_fullStr	Viral Diversity of House Mice in New York City
title_full_unstemmed	Viral Diversity of House Mice in New York City
title_sort	viral diversity of house mice in new york city
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/16ce31fd5afd402e9260af65e027c78d
work_keys_str_mv	AT simonhwilliams viraldiversityofhousemiceinnewyorkcity AT xiaoyuche viraldiversityofhousemiceinnewyorkcity AT joelagarcia viraldiversityofhousemiceinnewyorkcity AT johndklena viraldiversityofhousemiceinnewyorkcity AT bohyunlee viraldiversityofhousemiceinnewyorkcity AT dorothymuller viraldiversityofhousemiceinnewyorkcity AT wernerulrich viraldiversityofhousemiceinnewyorkcity AT robertmcorrigan viraldiversityofhousemiceinnewyorkcity AT stuartnichol viraldiversityofhousemiceinnewyorkcity AT komaljain viraldiversityofhousemiceinnewyorkcity AT wianlipkin viraldiversityofhousemiceinnewyorkcity
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Viral Diversity of House Mice in New York City

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