Early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury

Abstract The present study aimed to investigate the interaction between early diabetes and renal IR-induced AKI and to clarify the mechanisms involved. C57BL/6J mice were assigned to the following groups: (1) sham-operated; (2) renal IR; (3) streptozotocin (STZ—55 mg/kg/day) and sham operation; and...

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Autores principales:	Mariana Charleaux de Ponte, Vanessa Gerolde Cardoso, Guilherme Lopes Gonçalves, Juliana Martins Costa-Pessoa, Maria Oliveira-Souza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16d8c6a9b48e40acbc54a6ce18659f37
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spelling	oai:doaj.org-article:16d8c6a9b48e40acbc54a6ce18659f372021-12-02T18:48:09ZEarly type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury10.1038/s41598-021-97839-72045-2322https://doaj.org/article/16d8c6a9b48e40acbc54a6ce18659f372021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97839-7https://doaj.org/toc/2045-2322Abstract The present study aimed to investigate the interaction between early diabetes and renal IR-induced AKI and to clarify the mechanisms involved. C57BL/6J mice were assigned to the following groups: (1) sham-operated; (2) renal IR; (3) streptozotocin (STZ—55 mg/kg/day) and sham operation; and (4) STZ and renal IR. On the 12th day after treatments, the animals were subjected to bilateral IR for 30 min followed by reperfusion for 48 h, at which time the animals were euthanized. Renal function was assessed by plasma creatinine and urea levels, as well urinary protein contents. Kidney morphology and gene and protein expression were also evaluated. Compared to the sham group, renal IR increased plasma creatinine, urea and albuminuria levels and decreased Nphs1 mRNA expression and nephrin and WT1 protein staining. Tubular injury was observed with increased Havcr1 and Mki67 mRNA expression accompanied by reduced megalin staining. Renal IR also resulted in increased SQSTM1 protein expression and increased proinflammatory and profibrotic factors mRNA expression. Although STZ treatment resulted in hyperglycemia, it did not induce significant changes in renal function. On the other hand, STZ treatment aggravated renal IR-induced AKI by exacerbating renal dysfunction, glomerular and tubular injury, inflammation, and profibrotic responses. Thus, early diabetes constitutes a relevant risk factor for renal IR-induced AKI.Mariana Charleaux de PonteVanessa Gerolde CardosoGuilherme Lopes GonçalvesJuliana Martins Costa-PessoaMaria Oliveira-SouzaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariana Charleaux de Ponte Vanessa Gerolde Cardoso Guilherme Lopes Gonçalves Juliana Martins Costa-Pessoa Maria Oliveira-Souza Early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury
description	Abstract The present study aimed to investigate the interaction between early diabetes and renal IR-induced AKI and to clarify the mechanisms involved. C57BL/6J mice were assigned to the following groups: (1) sham-operated; (2) renal IR; (3) streptozotocin (STZ—55 mg/kg/day) and sham operation; and (4) STZ and renal IR. On the 12th day after treatments, the animals were subjected to bilateral IR for 30 min followed by reperfusion for 48 h, at which time the animals were euthanized. Renal function was assessed by plasma creatinine and urea levels, as well urinary protein contents. Kidney morphology and gene and protein expression were also evaluated. Compared to the sham group, renal IR increased plasma creatinine, urea and albuminuria levels and decreased Nphs1 mRNA expression and nephrin and WT1 protein staining. Tubular injury was observed with increased Havcr1 and Mki67 mRNA expression accompanied by reduced megalin staining. Renal IR also resulted in increased SQSTM1 protein expression and increased proinflammatory and profibrotic factors mRNA expression. Although STZ treatment resulted in hyperglycemia, it did not induce significant changes in renal function. On the other hand, STZ treatment aggravated renal IR-induced AKI by exacerbating renal dysfunction, glomerular and tubular injury, inflammation, and profibrotic responses. Thus, early diabetes constitutes a relevant risk factor for renal IR-induced AKI.
format	article
author	Mariana Charleaux de Ponte Vanessa Gerolde Cardoso Guilherme Lopes Gonçalves Juliana Martins Costa-Pessoa Maria Oliveira-Souza
author_facet	Mariana Charleaux de Ponte Vanessa Gerolde Cardoso Guilherme Lopes Gonçalves Juliana Martins Costa-Pessoa Maria Oliveira-Souza
author_sort	Mariana Charleaux de Ponte
title	Early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury
title_short	Early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury
title_full	Early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury
title_fullStr	Early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury
title_full_unstemmed	Early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury
title_sort	early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/16d8c6a9b48e40acbc54a6ce18659f37
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Early type 1 diabetes aggravates renal ischemia/reperfusion-induced acute kidney injury

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