Capturing the surface texture and shape of pollen: a comparison of microscopy techniques.

Research on the comparative morphology of pollen grains depends crucially on the application of appropriate microscopy techniques. Information on the performance of microscopy techniques can be used to inform that choice. We compared the ability of several microscopy techniques to provide informatio...

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Autores principales:	Mayandi Sivaguru, Luke Mander, Glenn Fried, Surangi W Punyasena
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16ddf6abff094b0abbd7f6abd548b294
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spelling	oai:doaj.org-article:16ddf6abff094b0abbd7f6abd548b2942021-11-18T07:15:41ZCapturing the surface texture and shape of pollen: a comparison of microscopy techniques.1932-620310.1371/journal.pone.0039129https://doaj.org/article/16ddf6abff094b0abbd7f6abd548b2942012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22720050/?tool=EBIhttps://doaj.org/toc/1932-6203Research on the comparative morphology of pollen grains depends crucially on the application of appropriate microscopy techniques. Information on the performance of microscopy techniques can be used to inform that choice. We compared the ability of several microscopy techniques to provide information on the shape and surface texture of three pollen types with differing morphologies. These techniques are: widefield, apotome, confocal and two-photon microscopy (reflected light techniques), and brightfield and differential interference contrast microscopy (DIC) (transmitted light techniques). We also provide a first view of pollen using super-resolution microscopy. The three pollen types used to contrast the performance of each technique are: Croton hirtus (Euphorbiaceae), Mabea occidentalis (Euphorbiaceae) and Agropyron repens (Poaceae). No single microscopy technique provided an adequate picture of both the shape and surface texture of any of the three pollen types investigated here. The wavelength of incident light, photon-collection ability of the optical technique, signal-to-noise ratio, and the thickness and light absorption characteristics of the exine profoundly affect the recovery of morphological information by a given optical microscopy technique. Reflected light techniques, particularly confocal and two-photon microscopy, best capture pollen shape but provide limited information on very fine surface texture. In contrast, transmitted light techniques, particularly differential interference contrast microscopy, can resolve very fine surface texture but provide limited information on shape. Texture comprising sculptural elements that are spaced near the diffraction limit of light (~250 nm; NDL) presents an acute challenge to optical microscopy. Super-resolution structured illumination microscopy provides data on the NDL texture of A. repens that is more comparable to textural data from scanning electron microscopy than any other optical microscopy technique investigated here. Maximizing the recovery of morphological information from pollen grains should lead to more robust classifications, and an increase in the taxonomic precision with which ancient vegetation can be reconstructed.Mayandi SivaguruLuke ManderGlenn FriedSurangi W PunyasenaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e39129 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mayandi Sivaguru Luke Mander Glenn Fried Surangi W Punyasena Capturing the surface texture and shape of pollen: a comparison of microscopy techniques.
description	Research on the comparative morphology of pollen grains depends crucially on the application of appropriate microscopy techniques. Information on the performance of microscopy techniques can be used to inform that choice. We compared the ability of several microscopy techniques to provide information on the shape and surface texture of three pollen types with differing morphologies. These techniques are: widefield, apotome, confocal and two-photon microscopy (reflected light techniques), and brightfield and differential interference contrast microscopy (DIC) (transmitted light techniques). We also provide a first view of pollen using super-resolution microscopy. The three pollen types used to contrast the performance of each technique are: Croton hirtus (Euphorbiaceae), Mabea occidentalis (Euphorbiaceae) and Agropyron repens (Poaceae). No single microscopy technique provided an adequate picture of both the shape and surface texture of any of the three pollen types investigated here. The wavelength of incident light, photon-collection ability of the optical technique, signal-to-noise ratio, and the thickness and light absorption characteristics of the exine profoundly affect the recovery of morphological information by a given optical microscopy technique. Reflected light techniques, particularly confocal and two-photon microscopy, best capture pollen shape but provide limited information on very fine surface texture. In contrast, transmitted light techniques, particularly differential interference contrast microscopy, can resolve very fine surface texture but provide limited information on shape. Texture comprising sculptural elements that are spaced near the diffraction limit of light (~250 nm; NDL) presents an acute challenge to optical microscopy. Super-resolution structured illumination microscopy provides data on the NDL texture of A. repens that is more comparable to textural data from scanning electron microscopy than any other optical microscopy technique investigated here. Maximizing the recovery of morphological information from pollen grains should lead to more robust classifications, and an increase in the taxonomic precision with which ancient vegetation can be reconstructed.
format	article
author	Mayandi Sivaguru Luke Mander Glenn Fried Surangi W Punyasena
author_facet	Mayandi Sivaguru Luke Mander Glenn Fried Surangi W Punyasena
author_sort	Mayandi Sivaguru
title	Capturing the surface texture and shape of pollen: a comparison of microscopy techniques.
title_short	Capturing the surface texture and shape of pollen: a comparison of microscopy techniques.
title_full	Capturing the surface texture and shape of pollen: a comparison of microscopy techniques.
title_fullStr	Capturing the surface texture and shape of pollen: a comparison of microscopy techniques.
title_full_unstemmed	Capturing the surface texture and shape of pollen: a comparison of microscopy techniques.
title_sort	capturing the surface texture and shape of pollen: a comparison of microscopy techniques.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/16ddf6abff094b0abbd7f6abd548b294
work_keys_str_mv	AT mayandisivaguru capturingthesurfacetextureandshapeofpollenacomparisonofmicroscopytechniques AT lukemander capturingthesurfacetextureandshapeofpollenacomparisonofmicroscopytechniques AT glennfried capturingthesurfacetextureandshapeofpollenacomparisonofmicroscopytechniques AT surangiwpunyasena capturingthesurfacetextureandshapeofpollenacomparisonofmicroscopytechniques
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Capturing the surface texture and shape of pollen: a comparison of microscopy techniques.

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