Study on RNAi-based herbicide for Mikania micrantha

The invasive plant Mikania micrantha Kunth (M. micrantha) from South America poses a significant threat to the stability and biodiversity of ecosystems. However, an effective and economical method to control M. micrantha is still lacking. RNA interference (RNAi) has been widely studied and applied i...

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Autores principales:	Jiantao Mai, Lingling Liao, Rongsong Ling, Xiaolong Guo, Jingying Lin, Beixin Mo, Weizhao Chen, Yu Yu
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2021
Materias:	Invasive plant Mikania micrantha RNA interference Spray-induced gene silencing Chlorophyll a/b-binding protein Nucleic acid bioherbicide Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/16e58581e39b48e698c10cd4834367ec
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spelling	oai:doaj.org-article:16e58581e39b48e698c10cd4834367ec2021-11-30T04:16:29ZStudy on RNAi-based herbicide for Mikania micrantha2405-805X10.1016/j.synbio.2021.11.005https://doaj.org/article/16e58581e39b48e698c10cd4834367ec2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405805X21000739https://doaj.org/toc/2405-805XThe invasive plant Mikania micrantha Kunth (M. micrantha) from South America poses a significant threat to the stability and biodiversity of ecosystems. However, an effective and economical method to control M. micrantha is still lacking. RNA interference (RNAi) has been widely studied and applied in agriculture for trait improvement. Spray-induced gene silencing (SIGS) can produce RNAi silencing effects without introducing heritable modifications to the plant genome and is becoming a novel nontransformation strategy for plant protection. In this study, the genes encoding chlorophyll a/b-binding proteins were selected as targets of RNAi, based on high-throughput sequencing of M. micrantha transcriptome and bioinformatic analyses of sequence specificity. Three types of RNAi molecules, double-stranded RNA, RNAi nanomicrosphere, and short hairpin RNA (shRNA), with their corresponding short interfering RNA sequences were designed and synthesized for SIGS vector construction, from which each RNAi molecule was transcribed and extracted to be sprayed on M. micrantha leaves. Whereas water-treated control leaves remained green, leaves treated with RNAi molecules turned yellow and eventually wilted. Quantitative real-time PCR showed that the expression levels of target genes were significantly reduced in the RNAi-treated groups compared with those of the control, suggesting that all three types of RNAi herbicides effectively silenced the endogenous target genes, which are essential for the growth of M. micrantha. We also found that shRNA showed better silencing efficiency than the other two molecules. Taken together, our study successfully designed three types of RNAi-based herbicides that specifically silenced endogenous target genes and controlled the growth of M. micrantha. Moreover, we identified a gene family encoding chlorophyll a/b-binding proteins that is important for the growth and development of M. micrantha and could serve as potential targets for controlling the spread of M. micrantha.Jiantao MaiLingling LiaoRongsong LingXiaolong GuoJingying LinBeixin MoWeizhao ChenYu YuKeAi Communications Co., Ltd.articleInvasive plantMikania micranthaRNA interferenceSpray-induced gene silencingChlorophyll a/b-binding proteinNucleic acid bioherbicideBiotechnologyTP248.13-248.65Biology (General)QH301-705.5ENSynthetic and Systems Biotechnology, Vol 6, Iss 4, Pp 437-445 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Invasive plant Mikania micrantha RNA interference Spray-induced gene silencing Chlorophyll a/b-binding protein Nucleic acid bioherbicide Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
spellingShingle	Invasive plant Mikania micrantha RNA interference Spray-induced gene silencing Chlorophyll a/b-binding protein Nucleic acid bioherbicide Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 Jiantao Mai Lingling Liao Rongsong Ling Xiaolong Guo Jingying Lin Beixin Mo Weizhao Chen Yu Yu Study on RNAi-based herbicide for Mikania micrantha
description	The invasive plant Mikania micrantha Kunth (M. micrantha) from South America poses a significant threat to the stability and biodiversity of ecosystems. However, an effective and economical method to control M. micrantha is still lacking. RNA interference (RNAi) has been widely studied and applied in agriculture for trait improvement. Spray-induced gene silencing (SIGS) can produce RNAi silencing effects without introducing heritable modifications to the plant genome and is becoming a novel nontransformation strategy for plant protection. In this study, the genes encoding chlorophyll a/b-binding proteins were selected as targets of RNAi, based on high-throughput sequencing of M. micrantha transcriptome and bioinformatic analyses of sequence specificity. Three types of RNAi molecules, double-stranded RNA, RNAi nanomicrosphere, and short hairpin RNA (shRNA), with their corresponding short interfering RNA sequences were designed and synthesized for SIGS vector construction, from which each RNAi molecule was transcribed and extracted to be sprayed on M. micrantha leaves. Whereas water-treated control leaves remained green, leaves treated with RNAi molecules turned yellow and eventually wilted. Quantitative real-time PCR showed that the expression levels of target genes were significantly reduced in the RNAi-treated groups compared with those of the control, suggesting that all three types of RNAi herbicides effectively silenced the endogenous target genes, which are essential for the growth of M. micrantha. We also found that shRNA showed better silencing efficiency than the other two molecules. Taken together, our study successfully designed three types of RNAi-based herbicides that specifically silenced endogenous target genes and controlled the growth of M. micrantha. Moreover, we identified a gene family encoding chlorophyll a/b-binding proteins that is important for the growth and development of M. micrantha and could serve as potential targets for controlling the spread of M. micrantha.
format	article
author	Jiantao Mai Lingling Liao Rongsong Ling Xiaolong Guo Jingying Lin Beixin Mo Weizhao Chen Yu Yu
author_facet	Jiantao Mai Lingling Liao Rongsong Ling Xiaolong Guo Jingying Lin Beixin Mo Weizhao Chen Yu Yu
author_sort	Jiantao Mai
title	Study on RNAi-based herbicide for Mikania micrantha
title_short	Study on RNAi-based herbicide for Mikania micrantha
title_full	Study on RNAi-based herbicide for Mikania micrantha
title_fullStr	Study on RNAi-based herbicide for Mikania micrantha
title_full_unstemmed	Study on RNAi-based herbicide for Mikania micrantha
title_sort	study on rnai-based herbicide for mikania micrantha
publisher	KeAi Communications Co., Ltd.
publishDate	2021
url	https://doaj.org/article/16e58581e39b48e698c10cd4834367ec
work_keys_str_mv	AT jiantaomai studyonrnaibasedherbicideformikaniamicrantha AT linglingliao studyonrnaibasedherbicideformikaniamicrantha AT rongsongling studyonrnaibasedherbicideformikaniamicrantha AT xiaolongguo studyonrnaibasedherbicideformikaniamicrantha AT jingyinglin studyonrnaibasedherbicideformikaniamicrantha AT beixinmo studyonrnaibasedherbicideformikaniamicrantha AT weizhaochen studyonrnaibasedherbicideformikaniamicrantha AT yuyu studyonrnaibasedherbicideformikaniamicrantha
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Study on RNAi-based herbicide for Mikania micrantha

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