Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR.

<h4>Background</h4>Urinary tract infections (UTI) are frequent in outpatients. Fast pathogen identification is mandatory for shortening the time of discomfort and preventing serious complications. Urine culture needs up to 48 hours until pathogen identification. Consequently, the initial...

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Autores principales:	Lutz E Lehmann, Stefan Hauser, Thomas Malinka, Sven Klaschik, Stefan U Weber, Jens-Christian Schewe, Frank Stüber, Malte Book
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16ef1648735544f289af99f6d5a7c686
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spelling	oai:doaj.org-article:16ef1648735544f289af99f6d5a7c6862021-11-18T06:58:43ZRapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR.1932-620310.1371/journal.pone.0017146https://doaj.org/article/16ef1648735544f289af99f6d5a7c6862011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21359187/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Urinary tract infections (UTI) are frequent in outpatients. Fast pathogen identification is mandatory for shortening the time of discomfort and preventing serious complications. Urine culture needs up to 48 hours until pathogen identification. Consequently, the initial antibiotic regimen is empirical.<h4>Aim</h4>To evaluate the feasibility of qualitative urine pathogen identification by a commercially available real-time PCR blood pathogen test (SeptiFast®) and to compare the results with dipslide and microbiological culture.<h4>Design of study</h4>Pilot study with prospectively collected urine samples.<h4>Setting</h4>University hospital.<h4>Methods</h4>82 prospectively collected urine samples from 81 patients with suspected UTI were included. Dipslide urine culture was followed by microbiological pathogen identification in dipslide positive samples. In parallel, qualitative DNA based pathogen identification (SeptiFast®) was performed in all samples.<h4>Results</h4>61 samples were SeptiFast® positive, whereas 67 samples were dipslide culture positive. The inter-methodological concordance of positive and negative findings in the gram+, gram- and fungi sector was 371/410 (90%), 477/492 (97%) and 238/246 (97%), respectively. Sensitivity and specificity of the SeptiFast® test for the detection of an infection was 0.82 and 0.60, respectively. SeptiFast® pathogen identifications were available at least 43 hours prior to culture results.<h4>Conclusion</h4>The SeptiFast® platform identified bacterial DNA in urine specimens considerably faster compared to conventional culture. For UTI diagnosis sensitivity and specificity is limited by its present qualitative setup which does not allow pathogen quantification. Future quantitative assays may hold promise for PCR based UTI pathogen identification as a supplementation of conventional culture methods.Lutz E LehmannStefan HauserThomas MalinkaSven KlaschikStefan U WeberJens-Christian ScheweFrank StüberMalte BookPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e17146 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lutz E Lehmann Stefan Hauser Thomas Malinka Sven Klaschik Stefan U Weber Jens-Christian Schewe Frank Stüber Malte Book Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR.
description	<h4>Background</h4>Urinary tract infections (UTI) are frequent in outpatients. Fast pathogen identification is mandatory for shortening the time of discomfort and preventing serious complications. Urine culture needs up to 48 hours until pathogen identification. Consequently, the initial antibiotic regimen is empirical.<h4>Aim</h4>To evaluate the feasibility of qualitative urine pathogen identification by a commercially available real-time PCR blood pathogen test (SeptiFast®) and to compare the results with dipslide and microbiological culture.<h4>Design of study</h4>Pilot study with prospectively collected urine samples.<h4>Setting</h4>University hospital.<h4>Methods</h4>82 prospectively collected urine samples from 81 patients with suspected UTI were included. Dipslide urine culture was followed by microbiological pathogen identification in dipslide positive samples. In parallel, qualitative DNA based pathogen identification (SeptiFast®) was performed in all samples.<h4>Results</h4>61 samples were SeptiFast® positive, whereas 67 samples were dipslide culture positive. The inter-methodological concordance of positive and negative findings in the gram+, gram- and fungi sector was 371/410 (90%), 477/492 (97%) and 238/246 (97%), respectively. Sensitivity and specificity of the SeptiFast® test for the detection of an infection was 0.82 and 0.60, respectively. SeptiFast® pathogen identifications were available at least 43 hours prior to culture results.<h4>Conclusion</h4>The SeptiFast® platform identified bacterial DNA in urine specimens considerably faster compared to conventional culture. For UTI diagnosis sensitivity and specificity is limited by its present qualitative setup which does not allow pathogen quantification. Future quantitative assays may hold promise for PCR based UTI pathogen identification as a supplementation of conventional culture methods.
format	article
author	Lutz E Lehmann Stefan Hauser Thomas Malinka Sven Klaschik Stefan U Weber Jens-Christian Schewe Frank Stüber Malte Book
author_facet	Lutz E Lehmann Stefan Hauser Thomas Malinka Sven Klaschik Stefan U Weber Jens-Christian Schewe Frank Stüber Malte Book
author_sort	Lutz E Lehmann
title	Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR.
title_short	Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR.
title_full	Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR.
title_fullStr	Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR.
title_full_unstemmed	Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR.
title_sort	rapid qualitative urinary tract infection pathogen identification by septifast real-time pcr.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/16ef1648735544f289af99f6d5a7c686
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Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR.

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