Improved molecular fingerprint analysis employing multi-branched gold nanoparticles in conjunction with surface-enhanced Raman scattering

Jencilin Johnston,1 Erik N Taylor,1,2 Richard J Gilbert,2 Thomas J Webster1,3 1Department of Chemical Engineering, 2Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA; 3Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi...

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Autores principales:	Johnston J, Taylor EN, Gilbert RJ, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Surface Enhanced Raman spectroscopy (SERS) Multi-branched gold nanoparticles gold nanospheres Raman tags IR820 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/16ef2f9f2cb4427bb5dc8ae0956f6c41
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spelling	oai:doaj.org-article:16ef2f9f2cb4427bb5dc8ae0956f6c412021-12-02T08:20:27ZImproved molecular fingerprint analysis employing multi-branched gold nanoparticles in conjunction with surface-enhanced Raman scattering1178-2013https://doaj.org/article/16ef2f9f2cb4427bb5dc8ae0956f6c412015-12-01T00:00:00Zhttps://www.dovepress.com/improved-molecular-fingerprint-analysis-employing-multi-branched-gold--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jencilin Johnston,1 Erik N Taylor,1,2 Richard J Gilbert,2 Thomas J Webster1,3 1Department of Chemical Engineering, 2Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA; 3Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Vibrational spectroscopy is a powerful analytical tool that assesses molecular properties based on spectroscopic signatures. In this study, the effect of gold nanoparticle morphology (spherical vs multi-branched) was assessed for the characterization of a Raman signal (ie, molecular fingerprint) that may be helpful for numerous medical applications. Multi-branched gold nanoparticles (MBAuNPs) were fabricated using a green chemistry method which employed the reduction of gold ion solute by 2-[4-(2-hydroxyethyl)-1-piperazyl] ethane sulfonic acid. Two types of reporter dyes, indocyanine (IR820 and IR792) and carbocyanine (DTTC [3,3'-diethylthiatricarbocyanine iodide] and DTDC [3,3'-diethylthiadicarbocyanine iodide]), were functionalized to the surface of the MBAuNPs and stabilized with denatured bovine serum albumin, thus forming the surface-enhanced Raman spectroscopy tag. Fluorescein isothiocyanate-conjugated anti-epidermal growth factor receptor to the surface-enhanced Raman spectroscopy tags and the properties of the resulting conjugates were assessed through determination of the Raman signal. Using the MBAuNP Raman probes synthesized in this manner, we demonstrated that MBAuNP provided significantly more surface-enhanced Raman scattering signal when compared with the associated spherical gold nanoparticle of similar size and concentration. MBAuNP enhancements were retained in the surface-enhanced Raman spectroscopy tags complexed to anti-epidermal growth factor receptor, providing evidence that this could be a useful biological probe for enhanced Raman molecular fingerprinting. Furthermore, while utilizing IR820 as a novel reporter dye linked with MBAuNP, superior Raman signal fingerprint results were obtained. Such results provide significant promise for the use of MBAuNP in the detection of numerous diseases for which biologically specific surface markers exist. Keywords: gold nanostars, IR820, SERSJohnston JTaylor ENGilbert RJWebster TJDove Medical PressarticleSurface Enhanced Raman spectroscopy (SERS)Multi-branched gold nanoparticlesgold nanospheresRaman tagsIR820Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 45-53 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Surface Enhanced Raman spectroscopy (SERS) Multi-branched gold nanoparticles gold nanospheres Raman tags IR820 Medicine (General) R5-920
spellingShingle	Surface Enhanced Raman spectroscopy (SERS) Multi-branched gold nanoparticles gold nanospheres Raman tags IR820 Medicine (General) R5-920 Johnston J Taylor EN Gilbert RJ Webster TJ Improved molecular fingerprint analysis employing multi-branched gold nanoparticles in conjunction with surface-enhanced Raman scattering
description	Jencilin Johnston,1 Erik N Taylor,1,2 Richard J Gilbert,2 Thomas J Webster1,3 1Department of Chemical Engineering, 2Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA; 3Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Vibrational spectroscopy is a powerful analytical tool that assesses molecular properties based on spectroscopic signatures. In this study, the effect of gold nanoparticle morphology (spherical vs multi-branched) was assessed for the characterization of a Raman signal (ie, molecular fingerprint) that may be helpful for numerous medical applications. Multi-branched gold nanoparticles (MBAuNPs) were fabricated using a green chemistry method which employed the reduction of gold ion solute by 2-[4-(2-hydroxyethyl)-1-piperazyl] ethane sulfonic acid. Two types of reporter dyes, indocyanine (IR820 and IR792) and carbocyanine (DTTC [3,3'-diethylthiatricarbocyanine iodide] and DTDC [3,3'-diethylthiadicarbocyanine iodide]), were functionalized to the surface of the MBAuNPs and stabilized with denatured bovine serum albumin, thus forming the surface-enhanced Raman spectroscopy tag. Fluorescein isothiocyanate-conjugated anti-epidermal growth factor receptor to the surface-enhanced Raman spectroscopy tags and the properties of the resulting conjugates were assessed through determination of the Raman signal. Using the MBAuNP Raman probes synthesized in this manner, we demonstrated that MBAuNP provided significantly more surface-enhanced Raman scattering signal when compared with the associated spherical gold nanoparticle of similar size and concentration. MBAuNP enhancements were retained in the surface-enhanced Raman spectroscopy tags complexed to anti-epidermal growth factor receptor, providing evidence that this could be a useful biological probe for enhanced Raman molecular fingerprinting. Furthermore, while utilizing IR820 as a novel reporter dye linked with MBAuNP, superior Raman signal fingerprint results were obtained. Such results provide significant promise for the use of MBAuNP in the detection of numerous diseases for which biologically specific surface markers exist. Keywords: gold nanostars, IR820, SERS
format	article
author	Johnston J Taylor EN Gilbert RJ Webster TJ
author_facet	Johnston J Taylor EN Gilbert RJ Webster TJ
author_sort	Johnston J
title	Improved molecular fingerprint analysis employing multi-branched gold nanoparticles in conjunction with surface-enhanced Raman scattering
title_short	Improved molecular fingerprint analysis employing multi-branched gold nanoparticles in conjunction with surface-enhanced Raman scattering
title_full	Improved molecular fingerprint analysis employing multi-branched gold nanoparticles in conjunction with surface-enhanced Raman scattering
title_fullStr	Improved molecular fingerprint analysis employing multi-branched gold nanoparticles in conjunction with surface-enhanced Raman scattering
title_full_unstemmed	Improved molecular fingerprint analysis employing multi-branched gold nanoparticles in conjunction with surface-enhanced Raman scattering
title_sort	improved molecular fingerprint analysis employing multi-branched gold nanoparticles in conjunction with surface-enhanced raman scattering
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/16ef2f9f2cb4427bb5dc8ae0956f6c41
work_keys_str_mv	AT johnstonj improvedmolecularfingerprintanalysisemployingmultibranchedgoldnanoparticlesinconjunctionwithsurfaceenhancedramanscattering AT tayloren improvedmolecularfingerprintanalysisemployingmultibranchedgoldnanoparticlesinconjunctionwithsurfaceenhancedramanscattering AT gilbertrj improvedmolecularfingerprintanalysisemployingmultibranchedgoldnanoparticlesinconjunctionwithsurfaceenhancedramanscattering AT webstertj improvedmolecularfingerprintanalysisemployingmultibranchedgoldnanoparticlesinconjunctionwithsurfaceenhancedramanscattering
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Improved molecular fingerprint analysis employing multi-branched gold nanoparticles in conjunction with surface-enhanced Raman scattering

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