Chloroplast Genomic Resource of Paris for Species Discrimination

Abstract Paris is famous in China for its medicinal value and has been included in the Chinese Pharmacopoeia. Inaccurate identification of these species could confound their effective exploration, conservation, and domestication. Due to the plasticity of the morphological characteristics, correct id...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yun Song, Shaojun Wang, Yuanming Ding, Jin Xu, Ming Fu Li, Shuifang Zhu, Naizhong Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/16f74d201f2340b39296b2a64a520529
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:16f74d201f2340b39296b2a64a520529
record_format	dspace
spelling	oai:doaj.org-article:16f74d201f2340b39296b2a64a5205292021-12-02T12:32:46ZChloroplast Genomic Resource of Paris for Species Discrimination10.1038/s41598-017-02083-72045-2322https://doaj.org/article/16f74d201f2340b39296b2a64a5205292017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02083-7https://doaj.org/toc/2045-2322Abstract Paris is famous in China for its medicinal value and has been included in the Chinese Pharmacopoeia. Inaccurate identification of these species could confound their effective exploration, conservation, and domestication. Due to the plasticity of the morphological characteristics, correct identification among Paris species remains problematic. In this regard, we report the complete chloroplast genome of P. thibetica and P. rugosa to develop highly variable molecular markers. Comparing three chloroplast genomes, we sought out the most variable regions to develop the best cpDNA barcodes for Paris. The size of Paris chloroplast genome ranged from 162,708 to 163,200 bp. A total of 134 genes comprising 81 protein coding genes, 45 tRNA genes and 8 rRNA genes were observed in all three chloroplast genomes. Eight rapidly evolving regions were detected, as well as the difference of simple sequence repeats (SSR) and repeat sequence. Two regions of the coding gene ycf1, ycf1a and ycf1b, evolved the quickest and were proposed as core barcodes for Paris. The complete chloroplast genome sequences provide more integrated and adequate information for better understanding the phylogenetic pattern and improving efficient discrimination during species identification.Yun SongShaojun WangYuanming DingJin XuMing Fu LiShuifang ZhuNaizhong ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yun Song Shaojun Wang Yuanming Ding Jin Xu Ming Fu Li Shuifang Zhu Naizhong Chen Chloroplast Genomic Resource of Paris for Species Discrimination
description	Abstract Paris is famous in China for its medicinal value and has been included in the Chinese Pharmacopoeia. Inaccurate identification of these species could confound their effective exploration, conservation, and domestication. Due to the plasticity of the morphological characteristics, correct identification among Paris species remains problematic. In this regard, we report the complete chloroplast genome of P. thibetica and P. rugosa to develop highly variable molecular markers. Comparing three chloroplast genomes, we sought out the most variable regions to develop the best cpDNA barcodes for Paris. The size of Paris chloroplast genome ranged from 162,708 to 163,200 bp. A total of 134 genes comprising 81 protein coding genes, 45 tRNA genes and 8 rRNA genes were observed in all three chloroplast genomes. Eight rapidly evolving regions were detected, as well as the difference of simple sequence repeats (SSR) and repeat sequence. Two regions of the coding gene ycf1, ycf1a and ycf1b, evolved the quickest and were proposed as core barcodes for Paris. The complete chloroplast genome sequences provide more integrated and adequate information for better understanding the phylogenetic pattern and improving efficient discrimination during species identification.
format	article
author	Yun Song Shaojun Wang Yuanming Ding Jin Xu Ming Fu Li Shuifang Zhu Naizhong Chen
author_facet	Yun Song Shaojun Wang Yuanming Ding Jin Xu Ming Fu Li Shuifang Zhu Naizhong Chen
author_sort	Yun Song
title	Chloroplast Genomic Resource of Paris for Species Discrimination
title_short	Chloroplast Genomic Resource of Paris for Species Discrimination
title_full	Chloroplast Genomic Resource of Paris for Species Discrimination
title_fullStr	Chloroplast Genomic Resource of Paris for Species Discrimination
title_full_unstemmed	Chloroplast Genomic Resource of Paris for Species Discrimination
title_sort	chloroplast genomic resource of paris for species discrimination
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/16f74d201f2340b39296b2a64a520529
work_keys_str_mv	AT yunsong chloroplastgenomicresourceofparisforspeciesdiscrimination AT shaojunwang chloroplastgenomicresourceofparisforspeciesdiscrimination AT yuanmingding chloroplastgenomicresourceofparisforspeciesdiscrimination AT jinxu chloroplastgenomicresourceofparisforspeciesdiscrimination AT mingfuli chloroplastgenomicresourceofparisforspeciesdiscrimination AT shuifangzhu chloroplastgenomicresourceofparisforspeciesdiscrimination AT naizhongchen chloroplastgenomicresourceofparisforspeciesdiscrimination
_version_	1718393963634753536

Chloroplast Genomic Resource of Paris for Species Discrimination

Ejemplares similares