DIAGNOSTIC VALUE OF SPECIFIC IMMUNE COMPLEXES IN DETECTION OF ACTIVE TUBERCULOSIS INFECTION

The diagnostic of tuberculosis infection, including the use of immunological methods, evolved significant changes. The introduction of new diagnostic tests allowed to improve the diagnosis of latent tuberculosis infection (LTI). However, the positive results of immunological tests in both tuberculos...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	A. A. Starshinova, E. V. Istomina, Yu. S. Zinchenko, M. V. Filatov, S. B. Landa, V. S. Burdakov, E. N. Belyaeva, M. M. Nazarenko, N. V. Sapozhnikova, M. V. Pavlova, P. K. Yablonskiy
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	tuberculosis test with tuberculosis recombinant allergen immunological tests latent tuberculosis infection dynamic light scattering method Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/170504d5496a405aa526c80ad331fc71
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:170504d5496a405aa526c80ad331fc71
record_format	dspace
spelling	oai:doaj.org-article:170504d5496a405aa526c80ad331fc712021-11-18T08:03:48ZDIAGNOSTIC VALUE OF SPECIFIC IMMUNE COMPLEXES IN DETECTION OF ACTIVE TUBERCULOSIS INFECTION1563-06252313-741X10.15789/1563-0625-2019-2-269-278https://doaj.org/article/170504d5496a405aa526c80ad331fc712019-05-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1554https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe diagnostic of tuberculosis infection, including the use of immunological methods, evolved significant changes. The introduction of new diagnostic tests allowed to improve the diagnosis of latent tuberculosis infection (LTI). However, the positive results of immunological tests in both tuberculosis patients and in those with LTI do not allow to divide these conditions, which requires the development and implementation of new diagnostic approaches.Materials and methods. A prospective study with a survey of two groups of patients was conducted: group I (n=50) - patients with verified pulmonary tuberculosis, MBT (+); group II (n =15) – subjects with LTI and control group – healthy subjects (n=14). The examination complex included clinical, radiological, bacteriological, immunological (Mantoux test with 2 TU, T-SPOT.TB, QFT and Diaskintest) methods. Immune complexes were determined in all patients and healthy individuals by the method of dynamic light scattering after the in vitro addition of specific antigens - peptides ESAT-6 and SFP-10.Results. The obtained data demonstrate the low informativeness of the clinical method in the diagnostic of pulmonary tuberculosis. In the presence of characteristic X-ray changes, bacteriological verification of tuberculosis was obtained only in 46% of cases. The use of various immunological tests allows to obtain positive test results in 84-90% of cases simultaneously with the 100% of the positive results in subjects with LTI. Determination of specific immune complexes by the method of dynamic light scattering allows to determine the activity of tuberculosis infection in 100 % of cases and to identify a high-risk group for the development of active tuberculosis in people with latent tuberculosis infection.Conclusions: the obtained data can be applied not only in the diagnosis of active tuberculosis in the absence of diagnosis verification, but also allow to identify a high-risk group for the development of the disease in people with latent tuberculosis infection.A. A. StarshinovaE. V. IstominaYu. S. ZinchenkoM. V. FilatovS. B. LandaV. S. BurdakovE. N. BelyaevaM. M. NazarenkoN. V. SapozhnikovaM. V. PavlovaP. K. YablonskiySPb RAACIarticletuberculosistest with tuberculosis recombinant allergenimmunological testslatent tuberculosis infectiondynamic light scattering methodImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 2, Pp 269-278 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	tuberculosis test with tuberculosis recombinant allergen immunological tests latent tuberculosis infection dynamic light scattering method Immunologic diseases. Allergy RC581-607
spellingShingle	tuberculosis test with tuberculosis recombinant allergen immunological tests latent tuberculosis infection dynamic light scattering method Immunologic diseases. Allergy RC581-607 A. A. Starshinova E. V. Istomina Yu. S. Zinchenko M. V. Filatov S. B. Landa V. S. Burdakov E. N. Belyaeva M. M. Nazarenko N. V. Sapozhnikova M. V. Pavlova P. K. Yablonskiy DIAGNOSTIC VALUE OF SPECIFIC IMMUNE COMPLEXES IN DETECTION OF ACTIVE TUBERCULOSIS INFECTION
description	The diagnostic of tuberculosis infection, including the use of immunological methods, evolved significant changes. The introduction of new diagnostic tests allowed to improve the diagnosis of latent tuberculosis infection (LTI). However, the positive results of immunological tests in both tuberculosis patients and in those with LTI do not allow to divide these conditions, which requires the development and implementation of new diagnostic approaches.Materials and methods. A prospective study with a survey of two groups of patients was conducted: group I (n=50) - patients with verified pulmonary tuberculosis, MBT (+); group II (n =15) – subjects with LTI and control group – healthy subjects (n=14). The examination complex included clinical, radiological, bacteriological, immunological (Mantoux test with 2 TU, T-SPOT.TB, QFT and Diaskintest) methods. Immune complexes were determined in all patients and healthy individuals by the method of dynamic light scattering after the in vitro addition of specific antigens - peptides ESAT-6 and SFP-10.Results. The obtained data demonstrate the low informativeness of the clinical method in the diagnostic of pulmonary tuberculosis. In the presence of characteristic X-ray changes, bacteriological verification of tuberculosis was obtained only in 46% of cases. The use of various immunological tests allows to obtain positive test results in 84-90% of cases simultaneously with the 100% of the positive results in subjects with LTI. Determination of specific immune complexes by the method of dynamic light scattering allows to determine the activity of tuberculosis infection in 100 % of cases and to identify a high-risk group for the development of active tuberculosis in people with latent tuberculosis infection.Conclusions: the obtained data can be applied not only in the diagnosis of active tuberculosis in the absence of diagnosis verification, but also allow to identify a high-risk group for the development of the disease in people with latent tuberculosis infection.
format	article
author	A. A. Starshinova E. V. Istomina Yu. S. Zinchenko M. V. Filatov S. B. Landa V. S. Burdakov E. N. Belyaeva M. M. Nazarenko N. V. Sapozhnikova M. V. Pavlova P. K. Yablonskiy
author_facet	A. A. Starshinova E. V. Istomina Yu. S. Zinchenko M. V. Filatov S. B. Landa V. S. Burdakov E. N. Belyaeva M. M. Nazarenko N. V. Sapozhnikova M. V. Pavlova P. K. Yablonskiy
author_sort	A. A. Starshinova
title	DIAGNOSTIC VALUE OF SPECIFIC IMMUNE COMPLEXES IN DETECTION OF ACTIVE TUBERCULOSIS INFECTION
title_short	DIAGNOSTIC VALUE OF SPECIFIC IMMUNE COMPLEXES IN DETECTION OF ACTIVE TUBERCULOSIS INFECTION
title_full	DIAGNOSTIC VALUE OF SPECIFIC IMMUNE COMPLEXES IN DETECTION OF ACTIVE TUBERCULOSIS INFECTION
title_fullStr	DIAGNOSTIC VALUE OF SPECIFIC IMMUNE COMPLEXES IN DETECTION OF ACTIVE TUBERCULOSIS INFECTION
title_full_unstemmed	DIAGNOSTIC VALUE OF SPECIFIC IMMUNE COMPLEXES IN DETECTION OF ACTIVE TUBERCULOSIS INFECTION
title_sort	diagnostic value of specific immune complexes in detection of active tuberculosis infection
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/170504d5496a405aa526c80ad331fc71
work_keys_str_mv	AT aastarshinova diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection AT evistomina diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection AT yuszinchenko diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection AT mvfilatov diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection AT sblanda diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection AT vsburdakov diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection AT enbelyaeva diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection AT mmnazarenko diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection AT nvsapozhnikova diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection AT mvpavlova diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection AT pkyablonskiy diagnosticvalueofspecificimmunecomplexesindetectionofactivetuberculosisinfection
_version_	1718422334783619072

DIAGNOSTIC VALUE OF SPECIFIC IMMUNE COMPLEXES IN DETECTION OF ACTIVE TUBERCULOSIS INFECTION

Ejemplares similares