Stable expression of mtlD gene imparts multiple stress tolerance in finger millet.

Finger millet is susceptible to abiotic stresses, especially drought and salinity stress, in the field during seed germination and early stages of seedling development. Therefore developing stress tolerant finger millet plants combating drought, salinity and associated oxidative stress in these two...

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Autores principales:	Ramanna Hema, Ramu S Vemanna, Shivakumar Sreeramulu, Chandrasekhara P Reddy, Muthappa Senthil-Kumar, Makarla Udayakumar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1705830a5e3945338425a07870e92d87
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spelling	oai:doaj.org-article:1705830a5e3945338425a07870e92d872021-11-18T08:15:58ZStable expression of mtlD gene imparts multiple stress tolerance in finger millet.1932-620310.1371/journal.pone.0099110https://doaj.org/article/1705830a5e3945338425a07870e92d872014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24922513/?tool=EBIhttps://doaj.org/toc/1932-6203Finger millet is susceptible to abiotic stresses, especially drought and salinity stress, in the field during seed germination and early stages of seedling development. Therefore developing stress tolerant finger millet plants combating drought, salinity and associated oxidative stress in these two growth stages is important. Cellular protection through osmotic adjustment and efficient free radical scavenging ability during abiotic stress are important components of stress tolerance mechanisms in plants. Mannitol, an osmolyte, is known to scavenge hydroxyl radicals generated during various abiotic stresses and thereby minimize stress damage in several plant species. In this study transgenic finger millet plants expressing the mannitol biosynthetic pathway gene from bacteria, mannitol-1-phosphate dehydrogenase (mtlD), were developed through Agrobacterium tumefaciens-mediated genetic transformation. mtlD gene integration in the putative transgenic plants was confirmed by Southern blot. Further, performance of transgenic finger millet under drought, salinity and oxidative stress was studied at plant level in T1 generation and in T1 and T2 generation seedlings. Results from these experiments showed that transgenic finger millet had better growth under drought and salinity stress compared to wild-type. At plant level, transgenic plants showed better osmotic adjustment and chlorophyll retention under drought stress compared to the wild-type. However, the overall increase in stress tolerance of transgenics for the three stresses, especially for oxidative stress, was only marginal compared to other mtlD gene expressing plant species reported in the literature. Moreover, the Agrobacterium-mediated genetic transformation protocol developed for finger millet in this study can be used to introduce diverse traits of agronomic importance in finger millet.Ramanna HemaRamu S VemannaShivakumar SreeramuluChandrasekhara P ReddyMuthappa Senthil-KumarMakarla UdayakumarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99110 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ramanna Hema Ramu S Vemanna Shivakumar Sreeramulu Chandrasekhara P Reddy Muthappa Senthil-Kumar Makarla Udayakumar Stable expression of mtlD gene imparts multiple stress tolerance in finger millet.
description	Finger millet is susceptible to abiotic stresses, especially drought and salinity stress, in the field during seed germination and early stages of seedling development. Therefore developing stress tolerant finger millet plants combating drought, salinity and associated oxidative stress in these two growth stages is important. Cellular protection through osmotic adjustment and efficient free radical scavenging ability during abiotic stress are important components of stress tolerance mechanisms in plants. Mannitol, an osmolyte, is known to scavenge hydroxyl radicals generated during various abiotic stresses and thereby minimize stress damage in several plant species. In this study transgenic finger millet plants expressing the mannitol biosynthetic pathway gene from bacteria, mannitol-1-phosphate dehydrogenase (mtlD), were developed through Agrobacterium tumefaciens-mediated genetic transformation. mtlD gene integration in the putative transgenic plants was confirmed by Southern blot. Further, performance of transgenic finger millet under drought, salinity and oxidative stress was studied at plant level in T1 generation and in T1 and T2 generation seedlings. Results from these experiments showed that transgenic finger millet had better growth under drought and salinity stress compared to wild-type. At plant level, transgenic plants showed better osmotic adjustment and chlorophyll retention under drought stress compared to the wild-type. However, the overall increase in stress tolerance of transgenics for the three stresses, especially for oxidative stress, was only marginal compared to other mtlD gene expressing plant species reported in the literature. Moreover, the Agrobacterium-mediated genetic transformation protocol developed for finger millet in this study can be used to introduce diverse traits of agronomic importance in finger millet.
format	article
author	Ramanna Hema Ramu S Vemanna Shivakumar Sreeramulu Chandrasekhara P Reddy Muthappa Senthil-Kumar Makarla Udayakumar
author_facet	Ramanna Hema Ramu S Vemanna Shivakumar Sreeramulu Chandrasekhara P Reddy Muthappa Senthil-Kumar Makarla Udayakumar
author_sort	Ramanna Hema
title	Stable expression of mtlD gene imparts multiple stress tolerance in finger millet.
title_short	Stable expression of mtlD gene imparts multiple stress tolerance in finger millet.
title_full	Stable expression of mtlD gene imparts multiple stress tolerance in finger millet.
title_fullStr	Stable expression of mtlD gene imparts multiple stress tolerance in finger millet.
title_full_unstemmed	Stable expression of mtlD gene imparts multiple stress tolerance in finger millet.
title_sort	stable expression of mtld gene imparts multiple stress tolerance in finger millet.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/1705830a5e3945338425a07870e92d87
work_keys_str_mv	AT ramannahema stableexpressionofmtldgeneimpartsmultiplestresstoleranceinfingermillet AT ramusvemanna stableexpressionofmtldgeneimpartsmultiplestresstoleranceinfingermillet AT shivakumarsreeramulu stableexpressionofmtldgeneimpartsmultiplestresstoleranceinfingermillet AT chandrasekharapreddy stableexpressionofmtldgeneimpartsmultiplestresstoleranceinfingermillet AT muthappasenthilkumar stableexpressionofmtldgeneimpartsmultiplestresstoleranceinfingermillet AT makarlaudayakumar stableexpressionofmtldgeneimpartsmultiplestresstoleranceinfingermillet
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Stable expression of mtlD gene imparts multiple stress tolerance in finger millet.

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