The role of polyamines in regulating amino acid biosynthesis in rice grains

Abstract Polyamines (PAs) are important endogenous plant growth regulators mediating the grain yield and quality in rice. This study was to clarify whether and how PAs regulate amino acid composition and content in milled rice. We grew three categories of rice cultivars differing in protein contents...

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Autores principales:	Yunji Xu, Chaoqun Jian, Ke Li, Yinfang Tian, Kuanyu Zhu, Weiyang Zhang, Weilu Wang, Zhiqin Wang, Jianchang Yang
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	amino acid biosynthesis rice (Oryza sativa L.) spermidine spermine Agriculture S Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/1708b4a10d1844cdafc45a94c2f96dff
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spelling	oai:doaj.org-article:1708b4a10d1844cdafc45a94c2f96dff2021-11-17T04:20:03ZThe role of polyamines in regulating amino acid biosynthesis in rice grains2048-369410.1002/fes3.306https://doaj.org/article/1708b4a10d1844cdafc45a94c2f96dff2021-11-01T00:00:00Zhttps://doi.org/10.1002/fes3.306https://doaj.org/toc/2048-3694Abstract Polyamines (PAs) are important endogenous plant growth regulators mediating the grain yield and quality in rice. This study was to clarify whether and how PAs regulate amino acid composition and content in milled rice. We grew three categories of rice cultivars differing in protein contents in the field and investigated the relationship of free‐PAs concentrations in filling grains with the activities of key enzymes involved in amino acid biosynthesis and with the amino acid contents of milled rice. Cultivars with higher concentrations of free‐Spermidine (Spd) and free‐Spermine (Spm) exhibited higher amino acid contents than the others. The concentrations of free‐Spd and free‐Spm were significantly and positively correlated with the contents of essential amino acids (EAAs), non‐essential amino acids (NEAAs), and total amino acids (TAAs) in milled rice, whereas the concentration of free‐Putrescine (Put) had no effect on these parameters. The concentrations of free‐Spd and free‐Spm were linearly associated with the activities of glutamine synthetase (GS), glutamate synthetase (GOGAT), aspartate transaminase (AST), aspartate kinase (AK), and alanine transaminase (ALT), but only GOGAT, AST, and ALT were significantly and positively correlated with amino acid content. The application of exogenous Spd or Spm to panicles increased the activities of these key enzymes and amino acid contents, whereas the application of MGBG (an inhibitor of Spd and Spm synthesis) had the opposite effect. Applying Put had no significant effect on these traits. These results suggest that free‐Spd and free‐Spm enhance amino acid biosynthesis during grain filling in rice, primarily by improving the activities of GOGAT, AST, and ALT.Yunji XuChaoqun JianKe LiYinfang TianKuanyu ZhuWeiyang ZhangWeilu WangZhiqin WangJianchang YangWileyarticleamino acidbiosynthesisrice (Oryza sativa L.)spermidinespermineAgricultureSAgriculture (General)S1-972ENFood and Energy Security, Vol 10, Iss 4, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	amino acid biosynthesis rice (Oryza sativa L.) spermidine spermine Agriculture S Agriculture (General) S1-972
spellingShingle	amino acid biosynthesis rice (Oryza sativa L.) spermidine spermine Agriculture S Agriculture (General) S1-972 Yunji Xu Chaoqun Jian Ke Li Yinfang Tian Kuanyu Zhu Weiyang Zhang Weilu Wang Zhiqin Wang Jianchang Yang The role of polyamines in regulating amino acid biosynthesis in rice grains
description	Abstract Polyamines (PAs) are important endogenous plant growth regulators mediating the grain yield and quality in rice. This study was to clarify whether and how PAs regulate amino acid composition and content in milled rice. We grew three categories of rice cultivars differing in protein contents in the field and investigated the relationship of free‐PAs concentrations in filling grains with the activities of key enzymes involved in amino acid biosynthesis and with the amino acid contents of milled rice. Cultivars with higher concentrations of free‐Spermidine (Spd) and free‐Spermine (Spm) exhibited higher amino acid contents than the others. The concentrations of free‐Spd and free‐Spm were significantly and positively correlated with the contents of essential amino acids (EAAs), non‐essential amino acids (NEAAs), and total amino acids (TAAs) in milled rice, whereas the concentration of free‐Putrescine (Put) had no effect on these parameters. The concentrations of free‐Spd and free‐Spm were linearly associated with the activities of glutamine synthetase (GS), glutamate synthetase (GOGAT), aspartate transaminase (AST), aspartate kinase (AK), and alanine transaminase (ALT), but only GOGAT, AST, and ALT were significantly and positively correlated with amino acid content. The application of exogenous Spd or Spm to panicles increased the activities of these key enzymes and amino acid contents, whereas the application of MGBG (an inhibitor of Spd and Spm synthesis) had the opposite effect. Applying Put had no significant effect on these traits. These results suggest that free‐Spd and free‐Spm enhance amino acid biosynthesis during grain filling in rice, primarily by improving the activities of GOGAT, AST, and ALT.
format	article
author	Yunji Xu Chaoqun Jian Ke Li Yinfang Tian Kuanyu Zhu Weiyang Zhang Weilu Wang Zhiqin Wang Jianchang Yang
author_facet	Yunji Xu Chaoqun Jian Ke Li Yinfang Tian Kuanyu Zhu Weiyang Zhang Weilu Wang Zhiqin Wang Jianchang Yang
author_sort	Yunji Xu
title	The role of polyamines in regulating amino acid biosynthesis in rice grains
title_short	The role of polyamines in regulating amino acid biosynthesis in rice grains
title_full	The role of polyamines in regulating amino acid biosynthesis in rice grains
title_fullStr	The role of polyamines in regulating amino acid biosynthesis in rice grains
title_full_unstemmed	The role of polyamines in regulating amino acid biosynthesis in rice grains
title_sort	role of polyamines in regulating amino acid biosynthesis in rice grains
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/1708b4a10d1844cdafc45a94c2f96dff
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The role of polyamines in regulating amino acid biosynthesis in rice grains

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