Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing

Abstract Tear fluid is receiving growing attention as a source for novel diagnostic biomarkers. Multiple techniques are available for its collection and impact the composition of acquired samples. We sought to provide a direct comparison of two collection methods with regard to implementation, accep...

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Autores principales:	Franziska Bachhuber, André Huss, Makbule Senel, Hayrettin Tumani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/170989ba04e1481ea84583e4bb9d0ea9
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spelling	oai:doaj.org-article:170989ba04e1481ea84583e4bb9d0ea92021-12-02T16:50:17ZDiagnostic biomarkers in tear fluid: from sampling to preanalytical processing10.1038/s41598-021-89514-82045-2322https://doaj.org/article/170989ba04e1481ea84583e4bb9d0ea92021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89514-8https://doaj.org/toc/2045-2322Abstract Tear fluid is receiving growing attention as a source for novel diagnostic biomarkers. Multiple techniques are available for its collection and impact the composition of acquired samples. We sought to provide a direct comparison of two collection methods with regard to implementation, acceptance, and impact on sample composition. Tear fluid was collected from fifteen healthy volunteers with capillary tubes and Schirmer strips and analyzed for total protein and IgG concentrations. Sampling parameters and perception by test persons were compared. The use of capillary tubes was more convenient for the participants while causing more effort for the collector. Tear flow rates as well as the relative and absolute amount of IgG were higher when Schirmer strips were used. Consecutive collections with Schirmer strips significantly influenced tear flow rates, IgG, and protein concentrations. A moderate correlation was observed between tear flow rates and IgG concentrations for both methods. Samples collected with both methods can be analyzed by isoelectric focusing, a potential diagnostic application in the field of neurology. The specific advantages and limitations of tear fluid sampling with either capillary tubes or Schirmer strips demonstrate the need for a thorough investigation of collection methods with regard to the application of interest.Franziska BachhuberAndré HussMakbule SenelHayrettin TumaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Franziska Bachhuber André Huss Makbule Senel Hayrettin Tumani Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing
description	Abstract Tear fluid is receiving growing attention as a source for novel diagnostic biomarkers. Multiple techniques are available for its collection and impact the composition of acquired samples. We sought to provide a direct comparison of two collection methods with regard to implementation, acceptance, and impact on sample composition. Tear fluid was collected from fifteen healthy volunteers with capillary tubes and Schirmer strips and analyzed for total protein and IgG concentrations. Sampling parameters and perception by test persons were compared. The use of capillary tubes was more convenient for the participants while causing more effort for the collector. Tear flow rates as well as the relative and absolute amount of IgG were higher when Schirmer strips were used. Consecutive collections with Schirmer strips significantly influenced tear flow rates, IgG, and protein concentrations. A moderate correlation was observed between tear flow rates and IgG concentrations for both methods. Samples collected with both methods can be analyzed by isoelectric focusing, a potential diagnostic application in the field of neurology. The specific advantages and limitations of tear fluid sampling with either capillary tubes or Schirmer strips demonstrate the need for a thorough investigation of collection methods with regard to the application of interest.
format	article
author	Franziska Bachhuber André Huss Makbule Senel Hayrettin Tumani
author_facet	Franziska Bachhuber André Huss Makbule Senel Hayrettin Tumani
author_sort	Franziska Bachhuber
title	Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing
title_short	Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing
title_full	Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing
title_fullStr	Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing
title_full_unstemmed	Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing
title_sort	diagnostic biomarkers in tear fluid: from sampling to preanalytical processing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/170989ba04e1481ea84583e4bb9d0ea9
work_keys_str_mv	AT franziskabachhuber diagnosticbiomarkersintearfluidfromsamplingtopreanalyticalprocessing AT andrehuss diagnosticbiomarkersintearfluidfromsamplingtopreanalyticalprocessing AT makbulesenel diagnosticbiomarkersintearfluidfromsamplingtopreanalyticalprocessing AT hayrettintumani diagnosticbiomarkersintearfluidfromsamplingtopreanalyticalprocessing
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Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing

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