Surface nanotopography mediated albumin adsorption, unfolding and modulation of early innate immune responses

Surface roughness plays an important role in regulating protein adsorption to biomaterial surfaces and modulating the subsequent inflammatory response. In this study, we examined the role of surface nanotopography on albumin adsorption, unfolding and subsequent immune responses. To achieve the objec...

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Autores principales:	Panthihage Ruvini L. Dabare, Akash Bachhuka, Emma Parkinson-Lawrence, Krasimir Vasilev
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Plasma polymerization Nanotopography Albumin adsorption Immune cells interaction Immune response Materials of engineering and construction. Mechanics of materials TA401-492
Acceso en línea:	https://doaj.org/article/170c2fcb659d4a25ae02563bef558ed0
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spelling	oai:doaj.org-article:170c2fcb659d4a25ae02563bef558ed02021-11-28T04:37:37ZSurface nanotopography mediated albumin adsorption, unfolding and modulation of early innate immune responses2590-049810.1016/j.mtadv.2021.100187https://doaj.org/article/170c2fcb659d4a25ae02563bef558ed02021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590049821000576https://doaj.org/toc/2590-0498Surface roughness plays an important role in regulating protein adsorption to biomaterial surfaces and modulating the subsequent inflammatory response. In this study, we examined the role of surface nanotopography on albumin adsorption, unfolding and subsequent immune responses. To achieve the objectives of the study, we create model surfaces of hill-like nanoprotrusions by covalently immobilizing gold nanoparticles (AuNPs) of predetermined sizes (16, 38, and 68 nm) on a functional plasma polymer layer. The amount of adsorbed albumin increased with the increase in surface area caused by greater surface nanotopography scales. Circular dichroism spectroscopy was used to evaluate albumin conformational changes and pointed to loss of α-helical structure on all model surfaces with the greatest conformational changes found on the smooth surface and the surface with largest nanotopography features. Studies with differentiated THP-1 cells (dTHP-1) demonstrated that immune cells interacted with surface adsorbed albumin via their scavenger receptors, which could bind to exposed peptide sequences caused by surface induced unfolding of the albumin. Pre-adsorption of albumin resulted in an overall decrease in the level of expression of pro-inflammatory cytokines from dTHP-1 cells. On the other hand, pre-adsorption of albumin led in an increase in the production of anti-inflammatory markers, which suggests a switch to the M2 pro-healing phenotype. The knowledge obtained from this study could instruct the design of healthcare materials where the generation of targeted surface nanotopography and pre-adsorption of albumin may enhance the biomaterial biocompatibility and lead to faster wound healing.Panthihage Ruvini L. DabareAkash BachhukaEmma Parkinson-LawrenceKrasimir VasilevElsevierarticlePlasma polymerizationNanotopographyAlbumin adsorptionImmune cells interactionImmune responseMaterials of engineering and construction. Mechanics of materialsTA401-492ENMaterials Today Advances, Vol 12, Iss , Pp 100187- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Plasma polymerization Nanotopography Albumin adsorption Immune cells interaction Immune response Materials of engineering and construction. Mechanics of materials TA401-492
spellingShingle	Plasma polymerization Nanotopography Albumin adsorption Immune cells interaction Immune response Materials of engineering and construction. Mechanics of materials TA401-492 Panthihage Ruvini L. Dabare Akash Bachhuka Emma Parkinson-Lawrence Krasimir Vasilev Surface nanotopography mediated albumin adsorption, unfolding and modulation of early innate immune responses
description	Surface roughness plays an important role in regulating protein adsorption to biomaterial surfaces and modulating the subsequent inflammatory response. In this study, we examined the role of surface nanotopography on albumin adsorption, unfolding and subsequent immune responses. To achieve the objectives of the study, we create model surfaces of hill-like nanoprotrusions by covalently immobilizing gold nanoparticles (AuNPs) of predetermined sizes (16, 38, and 68 nm) on a functional plasma polymer layer. The amount of adsorbed albumin increased with the increase in surface area caused by greater surface nanotopography scales. Circular dichroism spectroscopy was used to evaluate albumin conformational changes and pointed to loss of α-helical structure on all model surfaces with the greatest conformational changes found on the smooth surface and the surface with largest nanotopography features. Studies with differentiated THP-1 cells (dTHP-1) demonstrated that immune cells interacted with surface adsorbed albumin via their scavenger receptors, which could bind to exposed peptide sequences caused by surface induced unfolding of the albumin. Pre-adsorption of albumin resulted in an overall decrease in the level of expression of pro-inflammatory cytokines from dTHP-1 cells. On the other hand, pre-adsorption of albumin led in an increase in the production of anti-inflammatory markers, which suggests a switch to the M2 pro-healing phenotype. The knowledge obtained from this study could instruct the design of healthcare materials where the generation of targeted surface nanotopography and pre-adsorption of albumin may enhance the biomaterial biocompatibility and lead to faster wound healing.
format	article
author	Panthihage Ruvini L. Dabare Akash Bachhuka Emma Parkinson-Lawrence Krasimir Vasilev
author_facet	Panthihage Ruvini L. Dabare Akash Bachhuka Emma Parkinson-Lawrence Krasimir Vasilev
author_sort	Panthihage Ruvini L. Dabare
title	Surface nanotopography mediated albumin adsorption, unfolding and modulation of early innate immune responses
title_short	Surface nanotopography mediated albumin adsorption, unfolding and modulation of early innate immune responses
title_full	Surface nanotopography mediated albumin adsorption, unfolding and modulation of early innate immune responses
title_fullStr	Surface nanotopography mediated albumin adsorption, unfolding and modulation of early innate immune responses
title_full_unstemmed	Surface nanotopography mediated albumin adsorption, unfolding and modulation of early innate immune responses
title_sort	surface nanotopography mediated albumin adsorption, unfolding and modulation of early innate immune responses
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/170c2fcb659d4a25ae02563bef558ed0
work_keys_str_mv	AT panthihageruvinildabare surfacenanotopographymediatedalbuminadsorptionunfoldingandmodulationofearlyinnateimmuneresponses AT akashbachhuka surfacenanotopographymediatedalbuminadsorptionunfoldingandmodulationofearlyinnateimmuneresponses AT emmaparkinsonlawrence surfacenanotopographymediatedalbuminadsorptionunfoldingandmodulationofearlyinnateimmuneresponses AT krasimirvasilev surfacenanotopographymediatedalbuminadsorptionunfoldingandmodulationofearlyinnateimmuneresponses
_version_	1718408243301056512

Surface nanotopography mediated albumin adsorption, unfolding and modulation of early innate immune responses

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