Comparative NanoUPLC-MSE analysis between magainin I-susceptible and -resistant Escherichia coli strains

Abstract In recent years the antimicrobial peptides (AMPs) have been prospected and designed as new alternatives to conventional antibiotics. Indeed, AMPs have presented great potential toward pathogenic bacterial strains by means of complex mechanisms of action. However, reports have increasingly e...

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Autores principales:	Marlon H. Cardoso, Keyla C. de Almeida, Elizabete de S. Cândido, André M. Murad, Simoni C. Dias, Octávio L. Franco
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/170da4a763be4493a6aeadf0ff3829cf
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spelling	oai:doaj.org-article:170da4a763be4493a6aeadf0ff3829cf2021-12-02T12:32:00ZComparative NanoUPLC-MSE analysis between magainin I-susceptible and -resistant Escherichia coli strains10.1038/s41598-017-04181-y2045-2322https://doaj.org/article/170da4a763be4493a6aeadf0ff3829cf2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04181-yhttps://doaj.org/toc/2045-2322Abstract In recent years the antimicrobial peptides (AMPs) have been prospected and designed as new alternatives to conventional antibiotics. Indeed, AMPs have presented great potential toward pathogenic bacterial strains by means of complex mechanisms of action. However, reports have increasingly emerged regarding the mechanisms by which bacteria resist AMP administration. In this context, we performed a comparative proteomic study by using the total bacterial lysate of magainin I-susceptible and –resistant E. coli strains. After nanoUPLC-MSE analyses we identified 742 proteins distributed among the experimental groups, and 25 proteins were differentially expressed in the resistant strains. Among them 10 proteins involved in bacterial resistance, homeostasis, nutrition and protein transport were upregulated, while 15 proteins related to bacterial surface modifications, genetic information and β-lactams binding-protein were downregulated. Moreover, 60 exclusive proteins were identified in the resistant strains, among which biofilm and cell wall formation and multidrug efflux pump proteins could be observed. Thus, differentially from previous studies that could only associate single proteins to AMP bacterial resistance, data here reported show that several metabolic pathways may be related to E. coli resistance to AMPs, revealing the crucial role of multiple “omics” studies in order to elucidate the global molecular mechanisms involved in this resistance.Marlon H. CardosoKeyla C. de AlmeidaElizabete de S. CândidoAndré M. MuradSimoni C. DiasOctávio L. FrancoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marlon H. Cardoso Keyla C. de Almeida Elizabete de S. Cândido André M. Murad Simoni C. Dias Octávio L. Franco Comparative NanoUPLC-MSE analysis between magainin I-susceptible and -resistant Escherichia coli strains
description	Abstract In recent years the antimicrobial peptides (AMPs) have been prospected and designed as new alternatives to conventional antibiotics. Indeed, AMPs have presented great potential toward pathogenic bacterial strains by means of complex mechanisms of action. However, reports have increasingly emerged regarding the mechanisms by which bacteria resist AMP administration. In this context, we performed a comparative proteomic study by using the total bacterial lysate of magainin I-susceptible and –resistant E. coli strains. After nanoUPLC-MSE analyses we identified 742 proteins distributed among the experimental groups, and 25 proteins were differentially expressed in the resistant strains. Among them 10 proteins involved in bacterial resistance, homeostasis, nutrition and protein transport were upregulated, while 15 proteins related to bacterial surface modifications, genetic information and β-lactams binding-protein were downregulated. Moreover, 60 exclusive proteins were identified in the resistant strains, among which biofilm and cell wall formation and multidrug efflux pump proteins could be observed. Thus, differentially from previous studies that could only associate single proteins to AMP bacterial resistance, data here reported show that several metabolic pathways may be related to E. coli resistance to AMPs, revealing the crucial role of multiple “omics” studies in order to elucidate the global molecular mechanisms involved in this resistance.
format	article
author	Marlon H. Cardoso Keyla C. de Almeida Elizabete de S. Cândido André M. Murad Simoni C. Dias Octávio L. Franco
author_facet	Marlon H. Cardoso Keyla C. de Almeida Elizabete de S. Cândido André M. Murad Simoni C. Dias Octávio L. Franco
author_sort	Marlon H. Cardoso
title	Comparative NanoUPLC-MSE analysis between magainin I-susceptible and -resistant Escherichia coli strains
title_short	Comparative NanoUPLC-MSE analysis between magainin I-susceptible and -resistant Escherichia coli strains
title_full	Comparative NanoUPLC-MSE analysis between magainin I-susceptible and -resistant Escherichia coli strains
title_fullStr	Comparative NanoUPLC-MSE analysis between magainin I-susceptible and -resistant Escherichia coli strains
title_full_unstemmed	Comparative NanoUPLC-MSE analysis between magainin I-susceptible and -resistant Escherichia coli strains
title_sort	comparative nanouplc-mse analysis between magainin i-susceptible and -resistant escherichia coli strains
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/170da4a763be4493a6aeadf0ff3829cf
work_keys_str_mv	AT marlonhcardoso comparativenanouplcmseanalysisbetweenmagaininisusceptibleandresistantescherichiacolistrains AT keylacdealmeida comparativenanouplcmseanalysisbetweenmagaininisusceptibleandresistantescherichiacolistrains AT elizabetedescandido comparativenanouplcmseanalysisbetweenmagaininisusceptibleandresistantescherichiacolistrains AT andremmurad comparativenanouplcmseanalysisbetweenmagaininisusceptibleandresistantescherichiacolistrains AT simonicdias comparativenanouplcmseanalysisbetweenmagaininisusceptibleandresistantescherichiacolistrains AT octaviolfranco comparativenanouplcmseanalysisbetweenmagaininisusceptibleandresistantescherichiacolistrains
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Comparative NanoUPLC-MSE analysis between magainin I-susceptible and -resistant Escherichia coli strains

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