A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids

Plants synthesize more than 3000 tetrahydroisoquinoline (THIQ) alkaloids, but only a few of them have been produced by engineered microbes and titers are very low. Here, the authors increase (S)-reticuline titer to 4.6 g/L and repurpose the yeast Ehrlich pathway to synthesize a diverse array of THIQ...

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Autores principales:	Michael E. Pyne, Kaspar Kevvai, Parbir S. Grewal, Lauren Narcross, Brian Choi, Leanne Bourgeois, John E. Dueber, Vincent J. J. Martin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1711736c3c694987b81eeec5d825890d
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spelling	oai:doaj.org-article:1711736c3c694987b81eeec5d825890d2021-12-02T18:18:46ZA yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids10.1038/s41467-020-17172-x2041-1723https://doaj.org/article/1711736c3c694987b81eeec5d825890d2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17172-xhttps://doaj.org/toc/2041-1723Plants synthesize more than 3000 tetrahydroisoquinoline (THIQ) alkaloids, but only a few of them have been produced by engineered microbes and titers are very low. Here, the authors increase (S)-reticuline titer to 4.6 g/L and repurpose the yeast Ehrlich pathway to synthesize a diverse array of THIQ scaffolds.Michael E. PyneKaspar KevvaiParbir S. GrewalLauren NarcrossBrian ChoiLeanne BourgeoisJohn E. DueberVincent J. J. MartinNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Michael E. Pyne Kaspar Kevvai Parbir S. Grewal Lauren Narcross Brian Choi Leanne Bourgeois John E. Dueber Vincent J. J. Martin A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
description	Plants synthesize more than 3000 tetrahydroisoquinoline (THIQ) alkaloids, but only a few of them have been produced by engineered microbes and titers are very low. Here, the authors increase (S)-reticuline titer to 4.6 g/L and repurpose the yeast Ehrlich pathway to synthesize a diverse array of THIQ scaffolds.
format	article
author	Michael E. Pyne Kaspar Kevvai Parbir S. Grewal Lauren Narcross Brian Choi Leanne Bourgeois John E. Dueber Vincent J. J. Martin
author_facet	Michael E. Pyne Kaspar Kevvai Parbir S. Grewal Lauren Narcross Brian Choi Leanne Bourgeois John E. Dueber Vincent J. J. Martin
author_sort	Michael E. Pyne
title	A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
title_short	A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
title_full	A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
title_fullStr	A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
title_full_unstemmed	A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
title_sort	yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/1711736c3c694987b81eeec5d825890d
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A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids

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