CircRNA75 and CircRNA72 Function as the Sponge of MicroRNA-200 to Suppress Coelomocyte Apoptosis Via Targeting Tollip in Apostichopus japonicus

Circular RNAs (circRNAs) act as essential regulators in many biological processes, especially in mammalian immune response. Nonetheless, the functions and mechanisms of circRNAs in the invertebrate immune system are largely unclarified. In our previous work, 261 differentially expressed circRNAs pot...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiqing Liu, Xuelin Zhao, Xuemei Duan, Weiwei Zhang, Chenghua Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Apostichopus japonicus circRNA miR-200 Tollip apoptosis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/171c5b89da9b42ceb4ffeff0d35d1a57
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:171c5b89da9b42ceb4ffeff0d35d1a57
record_format	dspace
spelling	oai:doaj.org-article:171c5b89da9b42ceb4ffeff0d35d1a572021-11-17T04:49:46ZCircRNA75 and CircRNA72 Function as the Sponge of MicroRNA-200 to Suppress Coelomocyte Apoptosis Via Targeting Tollip in Apostichopus japonicus1664-322410.3389/fimmu.2021.770055https://doaj.org/article/171c5b89da9b42ceb4ffeff0d35d1a572021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.770055/fullhttps://doaj.org/toc/1664-3224Circular RNAs (circRNAs) act as essential regulators in many biological processes, especially in mammalian immune response. Nonetheless, the functions and mechanisms of circRNAs in the invertebrate immune system are largely unclarified. In our previous work, 261 differentially expressed circRNAs potentially related to the development of Apostichopus japonicus skin ulceration syndrome (SUS), which is a major problem restricting the sea cucumber breeding industry, were identified by genome-wide screening. In this study, via miRanda analysis, both circRNA75 and circrRNA72 were shown to share the miR-200 binding site, a key microRNA in the SUS. The two circRNAs were verified to be increased significantly in LPS-exposed primary coelomocytes, similar to the results of circRNA-seq in sea cucumber under Vibrio splendidus-challenged conditions. A dual-luciferase assay indicated that both circRNA75 and circRNA72 could bind miR-200 in vivo, in which circRNA75 had four binding sites of miR-200 and only one for circRNA72. Furthermore, we found that miR-200 could bind the 3’-UTR of Toll interacting protein (Tollip) to negatively mediate the expression of Tollip. Silencing Tollip increased primary coelomocyte apoptosis. Consistently, inference of circRNA75 and circRNA72 could also downregulate Tollip expression, thereby increasing the apoptosis of primary coelomocytes, which could be blocked by miR-200 inhibitor treatment. Moreover, the rate of si-circRNA75-downregulated Tollip expression was higher than that of si-circRNA72 under an equivalent amount. CircRNA75 and circRNA72 suppressed coelomocyte apoptosis by sponging miR-200 to promote Tollip expression. The ability of circRNA to adsorb miRNA might be positively related to the number of binding sites for miRNA.Jiqing LiuJiqing LiuXuelin ZhaoXuelin ZhaoXuemei DuanXuemei DuanWeiwei ZhangWeiwei ZhangChenghua LiChenghua LiChenghua LiFrontiers Media S.A.articleApostichopus japonicuscircRNAmiR-200TollipapoptosisImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Apostichopus japonicus circRNA miR-200 Tollip apoptosis Immunologic diseases. Allergy RC581-607
spellingShingle	Apostichopus japonicus circRNA miR-200 Tollip apoptosis Immunologic diseases. Allergy RC581-607 Jiqing Liu Jiqing Liu Xuelin Zhao Xuelin Zhao Xuemei Duan Xuemei Duan Weiwei Zhang Weiwei Zhang Chenghua Li Chenghua Li Chenghua Li CircRNA75 and CircRNA72 Function as the Sponge of MicroRNA-200 to Suppress Coelomocyte Apoptosis Via Targeting Tollip in Apostichopus japonicus
description	Circular RNAs (circRNAs) act as essential regulators in many biological processes, especially in mammalian immune response. Nonetheless, the functions and mechanisms of circRNAs in the invertebrate immune system are largely unclarified. In our previous work, 261 differentially expressed circRNAs potentially related to the development of Apostichopus japonicus skin ulceration syndrome (SUS), which is a major problem restricting the sea cucumber breeding industry, were identified by genome-wide screening. In this study, via miRanda analysis, both circRNA75 and circrRNA72 were shown to share the miR-200 binding site, a key microRNA in the SUS. The two circRNAs were verified to be increased significantly in LPS-exposed primary coelomocytes, similar to the results of circRNA-seq in sea cucumber under Vibrio splendidus-challenged conditions. A dual-luciferase assay indicated that both circRNA75 and circRNA72 could bind miR-200 in vivo, in which circRNA75 had four binding sites of miR-200 and only one for circRNA72. Furthermore, we found that miR-200 could bind the 3’-UTR of Toll interacting protein (Tollip) to negatively mediate the expression of Tollip. Silencing Tollip increased primary coelomocyte apoptosis. Consistently, inference of circRNA75 and circRNA72 could also downregulate Tollip expression, thereby increasing the apoptosis of primary coelomocytes, which could be blocked by miR-200 inhibitor treatment. Moreover, the rate of si-circRNA75-downregulated Tollip expression was higher than that of si-circRNA72 under an equivalent amount. CircRNA75 and circRNA72 suppressed coelomocyte apoptosis by sponging miR-200 to promote Tollip expression. The ability of circRNA to adsorb miRNA might be positively related to the number of binding sites for miRNA.
format	article
author	Jiqing Liu Jiqing Liu Xuelin Zhao Xuelin Zhao Xuemei Duan Xuemei Duan Weiwei Zhang Weiwei Zhang Chenghua Li Chenghua Li Chenghua Li
author_facet	Jiqing Liu Jiqing Liu Xuelin Zhao Xuelin Zhao Xuemei Duan Xuemei Duan Weiwei Zhang Weiwei Zhang Chenghua Li Chenghua Li Chenghua Li
author_sort	Jiqing Liu
title	CircRNA75 and CircRNA72 Function as the Sponge of MicroRNA-200 to Suppress Coelomocyte Apoptosis Via Targeting Tollip in Apostichopus japonicus
title_short	CircRNA75 and CircRNA72 Function as the Sponge of MicroRNA-200 to Suppress Coelomocyte Apoptosis Via Targeting Tollip in Apostichopus japonicus
title_full	CircRNA75 and CircRNA72 Function as the Sponge of MicroRNA-200 to Suppress Coelomocyte Apoptosis Via Targeting Tollip in Apostichopus japonicus
title_fullStr	CircRNA75 and CircRNA72 Function as the Sponge of MicroRNA-200 to Suppress Coelomocyte Apoptosis Via Targeting Tollip in Apostichopus japonicus
title_full_unstemmed	CircRNA75 and CircRNA72 Function as the Sponge of MicroRNA-200 to Suppress Coelomocyte Apoptosis Via Targeting Tollip in Apostichopus japonicus
title_sort	circrna75 and circrna72 function as the sponge of microrna-200 to suppress coelomocyte apoptosis via targeting tollip in apostichopus japonicus
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/171c5b89da9b42ceb4ffeff0d35d1a57
work_keys_str_mv	AT jiqingliu circrna75andcircrna72functionasthespongeofmicrorna200tosuppresscoelomocyteapoptosisviatargetingtollipinapostichopusjaponicus AT jiqingliu circrna75andcircrna72functionasthespongeofmicrorna200tosuppresscoelomocyteapoptosisviatargetingtollipinapostichopusjaponicus AT xuelinzhao circrna75andcircrna72functionasthespongeofmicrorna200tosuppresscoelomocyteapoptosisviatargetingtollipinapostichopusjaponicus AT xuelinzhao circrna75andcircrna72functionasthespongeofmicrorna200tosuppresscoelomocyteapoptosisviatargetingtollipinapostichopusjaponicus AT xuemeiduan circrna75andcircrna72functionasthespongeofmicrorna200tosuppresscoelomocyteapoptosisviatargetingtollipinapostichopusjaponicus AT xuemeiduan circrna75andcircrna72functionasthespongeofmicrorna200tosuppresscoelomocyteapoptosisviatargetingtollipinapostichopusjaponicus AT weiweizhang circrna75andcircrna72functionasthespongeofmicrorna200tosuppresscoelomocyteapoptosisviatargetingtollipinapostichopusjaponicus AT weiweizhang circrna75andcircrna72functionasthespongeofmicrorna200tosuppresscoelomocyteapoptosisviatargetingtollipinapostichopusjaponicus AT chenghuali circrna75andcircrna72functionasthespongeofmicrorna200tosuppresscoelomocyteapoptosisviatargetingtollipinapostichopusjaponicus AT chenghuali circrna75andcircrna72functionasthespongeofmicrorna200tosuppresscoelomocyteapoptosisviatargetingtollipinapostichopusjaponicus AT chenghuali circrna75andcircrna72functionasthespongeofmicrorna200tosuppresscoelomocyteapoptosisviatargetingtollipinapostichopusjaponicus
_version_	1718425972497186816

CircRNA75 and CircRNA72 Function as the Sponge of MicroRNA-200 to Suppress Coelomocyte Apoptosis Via Targeting Tollip in Apostichopus japonicus

Ejemplares similares