Systematic Analysis of the Lysine Crotonylome and Multiple Posttranslational Modification Analysis (Acetylation, Succinylation, and Crotonylation) in <named-content content-type="genus-species">Candida albicans</named-content>

ABSTRACT Candida albicans is an opportunistic pathogen that causes lethal fungal infections in immunocompromised patients. Lysine crotonylation is a newly discovered PTM (posttranslational modification) epigenetic type that may play a critical role in regulating gene expression. In this study, we us...

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Autores principales:	Xiaowei Zhou, Nana Song, Dongmei Li, Xiaofang Li, Weida Liu
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Candida albicans crotonylome histone lysine crotonylation motif PPI analysis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/171cad9f7cd44622bf5448d998faa5f6
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spelling	oai:doaj.org-article:171cad9f7cd44622bf5448d998faa5f62021-12-02T19:22:16ZSystematic Analysis of the Lysine Crotonylome and Multiple Posttranslational Modification Analysis (Acetylation, Succinylation, and Crotonylation) in <named-content content-type="genus-species">Candida albicans</named-content>10.1128/mSystems.01316-202379-5077https://doaj.org/article/171cad9f7cd44622bf5448d998faa5f62021-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.01316-20https://doaj.org/toc/2379-5077ABSTRACT Candida albicans is an opportunistic pathogen that causes lethal fungal infections in immunocompromised patients. Lysine crotonylation is a newly discovered PTM (posttranslational modification) epigenetic type that may play a critical role in regulating gene expression. In this study, we used an antibody-enrichment approach along with LC-MS/MS to carry out a quantitative crotonylome analysis in C. albicans. We found a total of 5,242 crotonylation sites and 1,584 crotonylated proteins among 9,038 proteins in this organism. Of these crotonylated proteins, a few unique crotonylated motifs are noted such as D and E in positions +1, +2, or +3 or K and R in positions +5 or +6, while A, E, F, G, P, W, and Y are in the −1 position or A, K, and R are found in positions −5, −6, −7, or −8. Functional analysis has shown that a majority of the crotonylated proteins are related to biosynthetic events and carbon metabolism. When combined with previously collected data on acetylation and succinylation, PPI (protein-protein interaction network) analysis reveals that proteins with functions in ribosomal biogenesis, oxidative phosphorylation, nucleus activity, and proteasome formation are heavily modified by these three PTM types. To the best of our knowledge, this is the first crotonylome study carried out in C. albicans and is an important step to a better understanding of the biological and pathogenic impact of PTM in C. albicans. IMPORTANCE C. albicans is a kind of pathogen of fungal infections that is found worldwide. Lysine crotonylation of proteins as a recently discovered PTM (posttranslational modification) may have a critical role in regulating cells. We first carried out large-scale analysis of crotonylated proteome and multiple PTM analysis (acetylation, succinylation, and crotonylation), then drew a diagram to show multiple PTM sites on histones in C. albicans of our study. This study about crotonylome in human pathogenic fungi is a milestone that first and deeply investigates the functional analysis of crotonylated proteins in C. albicans, which marks an important start for further research.Xiaowei ZhouNana SongDongmei LiXiaofang LiWeida LiuAmerican Society for MicrobiologyarticleCandida albicanscrotonylomehistonelysine crotonylation motifPPI analysisMicrobiologyQR1-502ENmSystems, Vol 6, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Candida albicans crotonylome histone lysine crotonylation motif PPI analysis Microbiology QR1-502
spellingShingle	Candida albicans crotonylome histone lysine crotonylation motif PPI analysis Microbiology QR1-502 Xiaowei Zhou Nana Song Dongmei Li Xiaofang Li Weida Liu Systematic Analysis of the Lysine Crotonylome and Multiple Posttranslational Modification Analysis (Acetylation, Succinylation, and Crotonylation) in <named-content content-type="genus-species">Candida albicans</named-content>
description	ABSTRACT Candida albicans is an opportunistic pathogen that causes lethal fungal infections in immunocompromised patients. Lysine crotonylation is a newly discovered PTM (posttranslational modification) epigenetic type that may play a critical role in regulating gene expression. In this study, we used an antibody-enrichment approach along with LC-MS/MS to carry out a quantitative crotonylome analysis in C. albicans. We found a total of 5,242 crotonylation sites and 1,584 crotonylated proteins among 9,038 proteins in this organism. Of these crotonylated proteins, a few unique crotonylated motifs are noted such as D and E in positions +1, +2, or +3 or K and R in positions +5 or +6, while A, E, F, G, P, W, and Y are in the −1 position or A, K, and R are found in positions −5, −6, −7, or −8. Functional analysis has shown that a majority of the crotonylated proteins are related to biosynthetic events and carbon metabolism. When combined with previously collected data on acetylation and succinylation, PPI (protein-protein interaction network) analysis reveals that proteins with functions in ribosomal biogenesis, oxidative phosphorylation, nucleus activity, and proteasome formation are heavily modified by these three PTM types. To the best of our knowledge, this is the first crotonylome study carried out in C. albicans and is an important step to a better understanding of the biological and pathogenic impact of PTM in C. albicans. IMPORTANCE C. albicans is a kind of pathogen of fungal infections that is found worldwide. Lysine crotonylation of proteins as a recently discovered PTM (posttranslational modification) may have a critical role in regulating cells. We first carried out large-scale analysis of crotonylated proteome and multiple PTM analysis (acetylation, succinylation, and crotonylation), then drew a diagram to show multiple PTM sites on histones in C. albicans of our study. This study about crotonylome in human pathogenic fungi is a milestone that first and deeply investigates the functional analysis of crotonylated proteins in C. albicans, which marks an important start for further research.
format	article
author	Xiaowei Zhou Nana Song Dongmei Li Xiaofang Li Weida Liu
author_facet	Xiaowei Zhou Nana Song Dongmei Li Xiaofang Li Weida Liu
author_sort	Xiaowei Zhou
title	Systematic Analysis of the Lysine Crotonylome and Multiple Posttranslational Modification Analysis (Acetylation, Succinylation, and Crotonylation) in <named-content content-type="genus-species">Candida albicans</named-content>
title_short	Systematic Analysis of the Lysine Crotonylome and Multiple Posttranslational Modification Analysis (Acetylation, Succinylation, and Crotonylation) in <named-content content-type="genus-species">Candida albicans</named-content>
title_full	Systematic Analysis of the Lysine Crotonylome and Multiple Posttranslational Modification Analysis (Acetylation, Succinylation, and Crotonylation) in <named-content content-type="genus-species">Candida albicans</named-content>
title_fullStr	Systematic Analysis of the Lysine Crotonylome and Multiple Posttranslational Modification Analysis (Acetylation, Succinylation, and Crotonylation) in <named-content content-type="genus-species">Candida albicans</named-content>
title_full_unstemmed	Systematic Analysis of the Lysine Crotonylome and Multiple Posttranslational Modification Analysis (Acetylation, Succinylation, and Crotonylation) in <named-content content-type="genus-species">Candida albicans</named-content>
title_sort	systematic analysis of the lysine crotonylome and multiple posttranslational modification analysis (acetylation, succinylation, and crotonylation) in <named-content content-type="genus-species">candida albicans</named-content>
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/171cad9f7cd44622bf5448d998faa5f6
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Systematic Analysis of the Lysine Crotonylome and Multiple Posttranslational Modification Analysis (Acetylation, Succinylation, and Crotonylation) in <named-content content-type="genus-species">Candida albicans</named-content>

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