Visualization of gold and platinum nanoparticles interacting with Salmonella Enteritidis and Listeria monocytogenes

Ewa Sawosz1, Andr&eacute; Chwalibog2, Jacek Szeliga3, Filip Sawosz2, Marta Grodzik1, Marlena Rupiewicz1, Tomasz Niemiec1, Katarzyna Kacprzyk11Division of Biotechnology and Biochemistry of Nutrition, Warsaw University of Life Sciences, Warsaw, Poland; 2Department of Basic Animal and Veterinar...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ewa Sawosz, Andr&eacute; Chwalibog, Jacek Szeliga, et al.
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/172b0a93b1f347df9f3505efc4f40e5f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:172b0a93b1f347df9f3505efc4f40e5f
record_format	dspace
spelling	oai:doaj.org-article:172b0a93b1f347df9f3505efc4f40e5f2021-12-02T02:49:27ZVisualization of gold and platinum nanoparticles interacting with Salmonella Enteritidis and Listeria monocytogenes1176-91141178-2013https://doaj.org/article/172b0a93b1f347df9f3505efc4f40e5f2010-08-01T00:00:00Zhttp://www.dovepress.com/visualization-of-gold-and-platinum-nanoparticles-interacting-with-salm-a5144https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ewa Sawosz1, Andr&eacute; Chwalibog2, Jacek Szeliga3, Filip Sawosz2, Marta Grodzik1, Marlena Rupiewicz1, Tomasz Niemiec1, Katarzyna Kacprzyk11Division of Biotechnology and Biochemistry of Nutrition, Warsaw University of Life Sciences, Warsaw, Poland; 2Department of Basic Animal and Veterinary Sciences, University of Copenhagen, Copenhagen, Denmark; 3Division of Microbiology of Analytical Centre, Warsaw University of Life Sciences, Warsaw, PolandPurpose: Rapid development of nanotechnology has recently brought significant attention to the extraordinary biological features of nanomaterials. The objective of the present &shy;investigation was to evaluate morphological characteristics of the assembles of gold and platinum nanoparticles (nano-Au and nano-Pt respectively), with Salmonella Enteritidis (Gram-negative) and Listeria monocytogenes (Gram-positive), to reveal possibilities of constructing bacteria-nanoparticle vehicles.Methods: Hydrocolloids of nano-Au or nano-Pt were added to two bacteria suspensions in the following order: nano-Au + Salmonella Enteritidis; nano-Au + Listeria monocytogenes; nano-Pt + Salmonella Enteritidis; nano-Pt + Listeria monocytogenes. Samples were inspected by transmission electron microscope.Results: Visualization of morphological interaction between nano-Au and Salmonella Enteritidis and Listeria monocytogenes, showed that nano-Au were aggregated within flagella or biofilm network and did not penetrate the bacterial cell. The analysis of morphological effects of interaction of nano-Pt with bacteria revealed that nano-Pt entered cells of Listeria monocytogenes and were removed from the cells. In the case of Salmonella Enteritidis, nano-Pt were seen inside bacteria cells, probably bound to DNA and partly left bacterial cells. After washing and centrifugation, some of the nano-Pt-DNA complexes were observed within Salmonella Enteritidis.Conclusion: The results indicate that the bacteria could be used as a vehicle to deliver nano-Pt to specific points in the body.Keywords: morphology, nanoparticles, gold, platinum, bacteria Ewa SawoszAndr&eacute; ChwalibogJacek Szeligaet al.Dove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 631-637 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ewa Sawosz Andr&eacute; Chwalibog Jacek Szeliga et al. Visualization of gold and platinum nanoparticles interacting with Salmonella Enteritidis and Listeria monocytogenes
description	Ewa Sawosz1, Andr&eacute; Chwalibog2, Jacek Szeliga3, Filip Sawosz2, Marta Grodzik1, Marlena Rupiewicz1, Tomasz Niemiec1, Katarzyna Kacprzyk11Division of Biotechnology and Biochemistry of Nutrition, Warsaw University of Life Sciences, Warsaw, Poland; 2Department of Basic Animal and Veterinary Sciences, University of Copenhagen, Copenhagen, Denmark; 3Division of Microbiology of Analytical Centre, Warsaw University of Life Sciences, Warsaw, PolandPurpose: Rapid development of nanotechnology has recently brought significant attention to the extraordinary biological features of nanomaterials. The objective of the present &shy;investigation was to evaluate morphological characteristics of the assembles of gold and platinum nanoparticles (nano-Au and nano-Pt respectively), with Salmonella Enteritidis (Gram-negative) and Listeria monocytogenes (Gram-positive), to reveal possibilities of constructing bacteria-nanoparticle vehicles.Methods: Hydrocolloids of nano-Au or nano-Pt were added to two bacteria suspensions in the following order: nano-Au + Salmonella Enteritidis; nano-Au + Listeria monocytogenes; nano-Pt + Salmonella Enteritidis; nano-Pt + Listeria monocytogenes. Samples were inspected by transmission electron microscope.Results: Visualization of morphological interaction between nano-Au and Salmonella Enteritidis and Listeria monocytogenes, showed that nano-Au were aggregated within flagella or biofilm network and did not penetrate the bacterial cell. The analysis of morphological effects of interaction of nano-Pt with bacteria revealed that nano-Pt entered cells of Listeria monocytogenes and were removed from the cells. In the case of Salmonella Enteritidis, nano-Pt were seen inside bacteria cells, probably bound to DNA and partly left bacterial cells. After washing and centrifugation, some of the nano-Pt-DNA complexes were observed within Salmonella Enteritidis.Conclusion: The results indicate that the bacteria could be used as a vehicle to deliver nano-Pt to specific points in the body.Keywords: morphology, nanoparticles, gold, platinum, bacteria
format	article
author	Ewa Sawosz Andr&eacute; Chwalibog Jacek Szeliga et al.
author_facet	Ewa Sawosz Andr&eacute; Chwalibog Jacek Szeliga et al.
author_sort	Ewa Sawosz
title	Visualization of gold and platinum nanoparticles interacting with Salmonella Enteritidis and Listeria monocytogenes
title_short	Visualization of gold and platinum nanoparticles interacting with Salmonella Enteritidis and Listeria monocytogenes
title_full	Visualization of gold and platinum nanoparticles interacting with Salmonella Enteritidis and Listeria monocytogenes
title_fullStr	Visualization of gold and platinum nanoparticles interacting with Salmonella Enteritidis and Listeria monocytogenes
title_full_unstemmed	Visualization of gold and platinum nanoparticles interacting with Salmonella Enteritidis and Listeria monocytogenes
title_sort	visualization of gold and platinum nanoparticles interacting with salmonella enteritidis and listeria monocytogenes
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/172b0a93b1f347df9f3505efc4f40e5f
work_keys_str_mv	AT ewasawosz visualizationofgoldandplatinumnanoparticlesinteractingwithsalmonellaenteritidisandlisteriamonocytogenes AT andrampeacutechwalibog visualizationofgoldandplatinumnanoparticlesinteractingwithsalmonellaenteritidisandlisteriamonocytogenes AT jacekszeliga visualizationofgoldandplatinumnanoparticlesinteractingwithsalmonellaenteritidisandlisteriamonocytogenes AT etal visualizationofgoldandplatinumnanoparticlesinteractingwithsalmonellaenteritidisandlisteriamonocytogenes
_version_	1718402086790496256

Visualization of gold and platinum nanoparticles interacting with Salmonella Enteritidis and Listeria monocytogenes

Ejemplares similares