Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye

Abstract Lysophosphatidylcholine (LPC)-induced demyelination is a versatile animal model that is frequently used to identify and examine molecular pathways of demyelination and remyelination in the central (CNS) and peripheral nervous system (PNS). However, identification of focally demyelinated les...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Reiji Yamazaki, Yasuyuki Osanai, Tom Kouki, Yoshiaki Shinohara, Jeffrey K. Huang, Nobuhiko Ohno
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/172e8f312b4e4b13b21153cfac8c51a6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:172e8f312b4e4b13b21153cfac8c51a6
record_format	dspace
spelling	oai:doaj.org-article:172e8f312b4e4b13b21153cfac8c51a62021-12-02T16:45:46ZMacroscopic detection of demyelinated lesions in mouse PNS with neutral red dye10.1038/s41598-021-96395-42045-2322https://doaj.org/article/172e8f312b4e4b13b21153cfac8c51a62021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96395-4https://doaj.org/toc/2045-2322Abstract Lysophosphatidylcholine (LPC)-induced demyelination is a versatile animal model that is frequently used to identify and examine molecular pathways of demyelination and remyelination in the central (CNS) and peripheral nervous system (PNS). However, identification of focally demyelinated lesion had been difficult and usually required tissue fixation, sectioning and histological analysis. Recently, a method for labeling and identification of demyelinated lesions in the CNS by intraperitoneal injection of neutral red (NR) dye was developed. However, it remained unknown whether NR can be used to label demyelinated lesions in PNS. In this study, we generated LPC-induced demyelination in sciatic nerve of mice, and demonstrated that the demyelinated lesions at the site of LPC injection were readily detectable at 7 days postlesion (dpl) by macroscopic observation of NR labeling. Moreover, NR staining gradually decreased from 7 to 21 dpl over the course of remyelination. Electron microscopy analysis of NR-labeled sciatic nerves at 7 dpl confirmed demyelination and myelin debris in lesions. Furthermore, fluorescence microscopy showed NR co-labeling with activated macrophages and Schwann cells in the PNS lesions. Together, NR labeling is a straightforward method that allows the macroscopic detection of demyelinated lesions in sciatic nerves after LPC injection.Reiji YamazakiYasuyuki OsanaiTom KoukiYoshiaki ShinoharaJeffrey K. HuangNobuhiko OhnoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Reiji Yamazaki Yasuyuki Osanai Tom Kouki Yoshiaki Shinohara Jeffrey K. Huang Nobuhiko Ohno Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye
description	Abstract Lysophosphatidylcholine (LPC)-induced demyelination is a versatile animal model that is frequently used to identify and examine molecular pathways of demyelination and remyelination in the central (CNS) and peripheral nervous system (PNS). However, identification of focally demyelinated lesion had been difficult and usually required tissue fixation, sectioning and histological analysis. Recently, a method for labeling and identification of demyelinated lesions in the CNS by intraperitoneal injection of neutral red (NR) dye was developed. However, it remained unknown whether NR can be used to label demyelinated lesions in PNS. In this study, we generated LPC-induced demyelination in sciatic nerve of mice, and demonstrated that the demyelinated lesions at the site of LPC injection were readily detectable at 7 days postlesion (dpl) by macroscopic observation of NR labeling. Moreover, NR staining gradually decreased from 7 to 21 dpl over the course of remyelination. Electron microscopy analysis of NR-labeled sciatic nerves at 7 dpl confirmed demyelination and myelin debris in lesions. Furthermore, fluorescence microscopy showed NR co-labeling with activated macrophages and Schwann cells in the PNS lesions. Together, NR labeling is a straightforward method that allows the macroscopic detection of demyelinated lesions in sciatic nerves after LPC injection.
format	article
author	Reiji Yamazaki Yasuyuki Osanai Tom Kouki Yoshiaki Shinohara Jeffrey K. Huang Nobuhiko Ohno
author_facet	Reiji Yamazaki Yasuyuki Osanai Tom Kouki Yoshiaki Shinohara Jeffrey K. Huang Nobuhiko Ohno
author_sort	Reiji Yamazaki
title	Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye
title_short	Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye
title_full	Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye
title_fullStr	Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye
title_full_unstemmed	Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye
title_sort	macroscopic detection of demyelinated lesions in mouse pns with neutral red dye
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/172e8f312b4e4b13b21153cfac8c51a6
work_keys_str_mv	AT reijiyamazaki macroscopicdetectionofdemyelinatedlesionsinmousepnswithneutralreddye AT yasuyukiosanai macroscopicdetectionofdemyelinatedlesionsinmousepnswithneutralreddye AT tomkouki macroscopicdetectionofdemyelinatedlesionsinmousepnswithneutralreddye AT yoshiakishinohara macroscopicdetectionofdemyelinatedlesionsinmousepnswithneutralreddye AT jeffreykhuang macroscopicdetectionofdemyelinatedlesionsinmousepnswithneutralreddye AT nobuhikoohno macroscopicdetectionofdemyelinatedlesionsinmousepnswithneutralreddye
_version_	1718383439696101376

Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye

Ejemplares similares