Rewiring of the FtsH regulatory network by a single nucleotide change in saeS of Staphylococcus aureus

Abstract In the Gram-positive pathogen Staphylococcus aureus, the membrane-bound ATP-dependent metalloprotease FtsH plays a critical role in resistance to various stressors. However, the molecular mechanism of the FtsH functions is not known. Here, we identified core FtsH target proteins in S. aureu...

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Autores principales:	Qian Liu, Mo Hu, Won-Sik Yeo, Lei He, Tianming Li, Yuanjun Zhu, Hongwei Meng, Yanan Wang, Hyunwoo Lee, Xiaoyun Liu, Min Li, Taeok Bae
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/172ed3d04f034637a863fc3a15066755
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spelling	oai:doaj.org-article:172ed3d04f034637a863fc3a150667552021-12-02T11:41:19ZRewiring of the FtsH regulatory network by a single nucleotide change in saeS of Staphylococcus aureus10.1038/s41598-017-08774-52045-2322https://doaj.org/article/172ed3d04f034637a863fc3a150667552017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08774-5https://doaj.org/toc/2045-2322Abstract In the Gram-positive pathogen Staphylococcus aureus, the membrane-bound ATP-dependent metalloprotease FtsH plays a critical role in resistance to various stressors. However, the molecular mechanism of the FtsH functions is not known. Here, we identified core FtsH target proteins in S. aureus. In the strains Newman and USA300, the abundance of 33 proteins were altered in both strains, of which 11 were identified as core FtsH substrate protein candidates. In the strain Newman and some other S. aureus strains, the sensor histidine kinase SaeS has an L18P (T53C in saeS) substitution, which transformed the protein into an FtsH substrate. Due to the increase of SaeS L18P in the ftsH mutant, Eap, a sae-regulon protein, was also increased in abundance, causing the Newman-specific cell-aggregation phenotype. Regardless of the strain background, however, the ftsH mutants showed lower virulence and survival in a murine infection model. Our study illustrates the elasticity of the bacterial regulatory network, which can be rewired by a single substitution mutation.Qian LiuMo HuWon-Sik YeoLei HeTianming LiYuanjun ZhuHongwei MengYanan WangHyunwoo LeeXiaoyun LiuMin LiTaeok BaeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qian Liu Mo Hu Won-Sik Yeo Lei He Tianming Li Yuanjun Zhu Hongwei Meng Yanan Wang Hyunwoo Lee Xiaoyun Liu Min Li Taeok Bae Rewiring of the FtsH regulatory network by a single nucleotide change in saeS of Staphylococcus aureus
description	Abstract In the Gram-positive pathogen Staphylococcus aureus, the membrane-bound ATP-dependent metalloprotease FtsH plays a critical role in resistance to various stressors. However, the molecular mechanism of the FtsH functions is not known. Here, we identified core FtsH target proteins in S. aureus. In the strains Newman and USA300, the abundance of 33 proteins were altered in both strains, of which 11 were identified as core FtsH substrate protein candidates. In the strain Newman and some other S. aureus strains, the sensor histidine kinase SaeS has an L18P (T53C in saeS) substitution, which transformed the protein into an FtsH substrate. Due to the increase of SaeS L18P in the ftsH mutant, Eap, a sae-regulon protein, was also increased in abundance, causing the Newman-specific cell-aggregation phenotype. Regardless of the strain background, however, the ftsH mutants showed lower virulence and survival in a murine infection model. Our study illustrates the elasticity of the bacterial regulatory network, which can be rewired by a single substitution mutation.
format	article
author	Qian Liu Mo Hu Won-Sik Yeo Lei He Tianming Li Yuanjun Zhu Hongwei Meng Yanan Wang Hyunwoo Lee Xiaoyun Liu Min Li Taeok Bae
author_facet	Qian Liu Mo Hu Won-Sik Yeo Lei He Tianming Li Yuanjun Zhu Hongwei Meng Yanan Wang Hyunwoo Lee Xiaoyun Liu Min Li Taeok Bae
author_sort	Qian Liu
title	Rewiring of the FtsH regulatory network by a single nucleotide change in saeS of Staphylococcus aureus
title_short	Rewiring of the FtsH regulatory network by a single nucleotide change in saeS of Staphylococcus aureus
title_full	Rewiring of the FtsH regulatory network by a single nucleotide change in saeS of Staphylococcus aureus
title_fullStr	Rewiring of the FtsH regulatory network by a single nucleotide change in saeS of Staphylococcus aureus
title_full_unstemmed	Rewiring of the FtsH regulatory network by a single nucleotide change in saeS of Staphylococcus aureus
title_sort	rewiring of the ftsh regulatory network by a single nucleotide change in saes of staphylococcus aureus
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/172ed3d04f034637a863fc3a15066755
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Rewiring of the FtsH regulatory network by a single nucleotide change in saeS of Staphylococcus aureus

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