Identification of postoperative prognostic microRNA predictors in hepatocellular carcinoma.

Comparison of microRNA (miRNA) expression profiles in the noncancerous liver tissues adjacent to hepatocelluar carcinomas (HCCs) was a strategy to identify postoperative prognostic predictors in this study. Expression profiles of 270 miRNAs were determined in the paraneoplastic liver tissues of 12 H...

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Autores principales:	Ya-Hui Huang, Kwang-Huei Lin, Hua-Chien Chen, Ming-Ling Chang, Chao-Wei Hsu, Ming-Wei Lai, Tse-Ching Chen, Wei-Chen Lee, Yi-Hsin Tseng, Chau-Ting Yeh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1735499cfb664e02a6aaa96b3e39b945
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spelling	oai:doaj.org-article:1735499cfb664e02a6aaa96b3e39b9452021-11-18T07:17:51ZIdentification of postoperative prognostic microRNA predictors in hepatocellular carcinoma.1932-620310.1371/journal.pone.0037188https://doaj.org/article/1735499cfb664e02a6aaa96b3e39b9452012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22629365/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Comparison of microRNA (miRNA) expression profiles in the noncancerous liver tissues adjacent to hepatocelluar carcinomas (HCCs) was a strategy to identify postoperative prognostic predictors in this study. Expression profiles of 270 miRNAs were determined in the paraneoplastic liver tissues of 12 HCC patients with known postoperative prognosis. A panel of candidate miRNA predictors was identified. The prognostic predictive value of these candidate miRNAs was then verified in 216 postoperative HCC patients. Univariate analysis identified 8 and 3 miRNA predictors for recurrence-free (RFS) and overall (OS) survivals, respectively. Multivariate analysis revealed high expression levels of miR-155 (HR, 2.002 [1.324-3.027]; P = .001), miR-15a (HR, 0.478 [0.248-0.920]; P = .027), miR-432 (HR, 1.816 [1.203-2.740]; P = .015), miR-486-3p (HR, 0.543 [0.330-0.893]; P = .016), miR-15b (HR, 1.074 [1.002-1.152]; P = .043) and miR-30b (HR, 1.102 [1.025-1.185]; P = .009) were significantly associated with RFS. When clinicopathological predictors were included, multivariate analysis revealed that tumor number and miR-432, miR-486-3p, and miR-30b expression levels remained significant as independent predictors for RFS. Additionally, expression knockdown of miR-155 in J7 and Mahlavu hepatoma cells resulted in decreased cell growth and enhanced cell death in xenograft tumors, suggesting an oncogenic effect of miR-155. In conclusion, significant prognostic miRNA predictors were identified through examination of miRNA expression levels in paraneoplastic liver tissues. Functional analysis of a miRNA predictor, miR-155, suggested that the prognostic miRNA predictors identified under this strategy could serve as potential molecular targets for anticancer therapy.Ya-Hui HuangKwang-Huei LinHua-Chien ChenMing-Ling ChangChao-Wei HsuMing-Wei LaiTse-Ching ChenWei-Chen LeeYi-Hsin TsengChau-Ting YehPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37188 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ya-Hui Huang Kwang-Huei Lin Hua-Chien Chen Ming-Ling Chang Chao-Wei Hsu Ming-Wei Lai Tse-Ching Chen Wei-Chen Lee Yi-Hsin Tseng Chau-Ting Yeh Identification of postoperative prognostic microRNA predictors in hepatocellular carcinoma.
description	Comparison of microRNA (miRNA) expression profiles in the noncancerous liver tissues adjacent to hepatocelluar carcinomas (HCCs) was a strategy to identify postoperative prognostic predictors in this study. Expression profiles of 270 miRNAs were determined in the paraneoplastic liver tissues of 12 HCC patients with known postoperative prognosis. A panel of candidate miRNA predictors was identified. The prognostic predictive value of these candidate miRNAs was then verified in 216 postoperative HCC patients. Univariate analysis identified 8 and 3 miRNA predictors for recurrence-free (RFS) and overall (OS) survivals, respectively. Multivariate analysis revealed high expression levels of miR-155 (HR, 2.002 [1.324-3.027]; P = .001), miR-15a (HR, 0.478 [0.248-0.920]; P = .027), miR-432 (HR, 1.816 [1.203-2.740]; P = .015), miR-486-3p (HR, 0.543 [0.330-0.893]; P = .016), miR-15b (HR, 1.074 [1.002-1.152]; P = .043) and miR-30b (HR, 1.102 [1.025-1.185]; P = .009) were significantly associated with RFS. When clinicopathological predictors were included, multivariate analysis revealed that tumor number and miR-432, miR-486-3p, and miR-30b expression levels remained significant as independent predictors for RFS. Additionally, expression knockdown of miR-155 in J7 and Mahlavu hepatoma cells resulted in decreased cell growth and enhanced cell death in xenograft tumors, suggesting an oncogenic effect of miR-155. In conclusion, significant prognostic miRNA predictors were identified through examination of miRNA expression levels in paraneoplastic liver tissues. Functional analysis of a miRNA predictor, miR-155, suggested that the prognostic miRNA predictors identified under this strategy could serve as potential molecular targets for anticancer therapy.
format	article
author	Ya-Hui Huang Kwang-Huei Lin Hua-Chien Chen Ming-Ling Chang Chao-Wei Hsu Ming-Wei Lai Tse-Ching Chen Wei-Chen Lee Yi-Hsin Tseng Chau-Ting Yeh
author_facet	Ya-Hui Huang Kwang-Huei Lin Hua-Chien Chen Ming-Ling Chang Chao-Wei Hsu Ming-Wei Lai Tse-Ching Chen Wei-Chen Lee Yi-Hsin Tseng Chau-Ting Yeh
author_sort	Ya-Hui Huang
title	Identification of postoperative prognostic microRNA predictors in hepatocellular carcinoma.
title_short	Identification of postoperative prognostic microRNA predictors in hepatocellular carcinoma.
title_full	Identification of postoperative prognostic microRNA predictors in hepatocellular carcinoma.
title_fullStr	Identification of postoperative prognostic microRNA predictors in hepatocellular carcinoma.
title_full_unstemmed	Identification of postoperative prognostic microRNA predictors in hepatocellular carcinoma.
title_sort	identification of postoperative prognostic microrna predictors in hepatocellular carcinoma.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1735499cfb664e02a6aaa96b3e39b945
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Identification of postoperative prognostic microRNA predictors in hepatocellular carcinoma.

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