Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers

Yuri D Ivanov,1 Natalia S Bukharina,1 Tatyana O Pleshakova,1 Pavel A Frantsuzov,1 Elena Yu Andreeva,1 Anna L Kaysheva,1,2 Victor G Zgoda,1 Alexander A Izotov,1 Tatyana I Pavlova,1 Vadim S Ziborov,1 Sergey P Radko,1 Sergei A Moshkovskii,1 Alexander I Archakov1 1Department of Personalized Medicine,...

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Autores principales:	Ivanov YD, Bukharina NS, Pleshakova TO, Frantsuzov PA, Andreeva EY, Kaysheva AL, Zgoda VG, Izotov AA, Pavlova TI, Ziborov VS, Radko SP, Moshkovskii SA, Archakov AI
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1737ed45e92e4620ba75b1a6a7c9e15c
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spelling	oai:doaj.org-article:1737ed45e92e4620ba75b1a6a7c9e15c2021-12-02T00:46:39ZAtomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers1178-2013https://doaj.org/article/1737ed45e92e4620ba75b1a6a7c9e15c2014-10-01T00:00:00Zhttp://www.dovepress.com/atomic-force-microscopy-fishing-and-mass-spectrometry-identification-o-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Yuri D Ivanov,1 Natalia S Bukharina,1 Tatyana O Pleshakova,1 Pavel A Frantsuzov,1 Elena Yu Andreeva,1 Anna L Kaysheva,1,2 Victor G Zgoda,1 Alexander A Izotov,1 Tatyana I Pavlova,1 Vadim S Ziborov,1 Sergey P Radko,1 Sergei A Moshkovskii,1 Alexander I Archakov1 1Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia; 2PostgenTech Ltd., Moscow, Russia Abstract: Atomic force microscopy (AFM) was applied to carry out direct and label-free detection of gp120 human immunodeficiency virus type 1 envelope glycoprotein as a target protein. This approach was based on the AFM fishing of gp120 from the analyte solution using anti-gp120 aptamers immobilized on the AFM chip to count gp120/aptamer complexes that were formed on the chip surface. The comparison of image contrasts of fished gp120 against the background of immobilized aptamers and anti-gp120 antibodies on the AFM images was conducted. It was shown that an image contrast of the protein/aptamer complexes was two-fold higher than the contrast of the protein/antibody complexes. Mass spectrometry identification provided an additional confirmation of the target protein presence on the AFM chips after biospecific fishing to avoid any artifacts. Keywords: gp120 HIV-1 envelope glycoprotein, aptamer, atomic force microscopy, mass spectrometryIvanov YDBukharina NSPleshakova TOFrantsuzov PAAndreeva EYKaysheva ALZgoda VGIzotov AAPavlova TIZiborov VSRadko SPMoshkovskii SAArchakov AIDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 4659-4670 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ivanov YD Bukharina NS Pleshakova TO Frantsuzov PA Andreeva EY Kaysheva AL Zgoda VG Izotov AA Pavlova TI Ziborov VS Radko SP Moshkovskii SA Archakov AI Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers
description	Yuri D Ivanov,1 Natalia S Bukharina,1 Tatyana O Pleshakova,1 Pavel A Frantsuzov,1 Elena Yu Andreeva,1 Anna L Kaysheva,1,2 Victor G Zgoda,1 Alexander A Izotov,1 Tatyana I Pavlova,1 Vadim S Ziborov,1 Sergey P Radko,1 Sergei A Moshkovskii,1 Alexander I Archakov1 1Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia; 2PostgenTech Ltd., Moscow, Russia Abstract: Atomic force microscopy (AFM) was applied to carry out direct and label-free detection of gp120 human immunodeficiency virus type 1 envelope glycoprotein as a target protein. This approach was based on the AFM fishing of gp120 from the analyte solution using anti-gp120 aptamers immobilized on the AFM chip to count gp120/aptamer complexes that were formed on the chip surface. The comparison of image contrasts of fished gp120 against the background of immobilized aptamers and anti-gp120 antibodies on the AFM images was conducted. It was shown that an image contrast of the protein/aptamer complexes was two-fold higher than the contrast of the protein/antibody complexes. Mass spectrometry identification provided an additional confirmation of the target protein presence on the AFM chips after biospecific fishing to avoid any artifacts. Keywords: gp120 HIV-1 envelope glycoprotein, aptamer, atomic force microscopy, mass spectrometry
format	article
author	Ivanov YD Bukharina NS Pleshakova TO Frantsuzov PA Andreeva EY Kaysheva AL Zgoda VG Izotov AA Pavlova TI Ziborov VS Radko SP Moshkovskii SA Archakov AI
author_facet	Ivanov YD Bukharina NS Pleshakova TO Frantsuzov PA Andreeva EY Kaysheva AL Zgoda VG Izotov AA Pavlova TI Ziborov VS Radko SP Moshkovskii SA Archakov AI
author_sort	Ivanov YD
title	Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers
title_short	Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers
title_full	Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers
title_fullStr	Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers
title_full_unstemmed	Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers
title_sort	atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/1737ed45e92e4620ba75b1a6a7c9e15c
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Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers

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