Designing a multi-epitope vaccine against Mycobacteroides abscessus by pangenome-reverse vaccinology

Abstract Mycobacteroides abscessus (Previously Mycobacterium abscessus) is an emerging microorganism of the newly defined genera Mycobacteroides that causes mainly skin and tissue diseases in humans. The recent availability of total 34 fully sequenced genomes of different strains belonging to this s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hamza Arshad Dar, Saba Ismail, Yasir Waheed, Sajjad Ahmad, Zubia Jamil, Hafsa Aziz, Helal F. Hetta, Khalid Muhammad
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/173e4f38ae614439a7fe47c9cb2fba38
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:173e4f38ae614439a7fe47c9cb2fba38
record_format	dspace
spelling	oai:doaj.org-article:173e4f38ae614439a7fe47c9cb2fba382021-12-02T14:49:17ZDesigning a multi-epitope vaccine against Mycobacteroides abscessus by pangenome-reverse vaccinology10.1038/s41598-021-90868-22045-2322https://doaj.org/article/173e4f38ae614439a7fe47c9cb2fba382021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90868-2https://doaj.org/toc/2045-2322Abstract Mycobacteroides abscessus (Previously Mycobacterium abscessus) is an emerging microorganism of the newly defined genera Mycobacteroides that causes mainly skin and tissue diseases in humans. The recent availability of total 34 fully sequenced genomes of different strains belonging to this species has provided an opportunity to utilize this genomics data to gain novel insights and guide the development of specific antimicrobial therapies. In the present study, we collected collectively 34 complete genome sequences of M. abscessus from the NCBI GenBank database. Pangenome analysis was conducted on these genomes to understand the genetic diversity and to obtain proteins associated with its core genome. These core proteins were then subjected to various subtractive filters to identify potential antigenic targets that were subjected to multi-epitope vaccine design. Our analysis projected the open pangenome of M. abscessus containing 3443 core genes. After applying various stepwise filtration steps on the core proteins, a total of four potential antigenic targets were identified. Utilizing their constituent CD4 and CD8 T-cell epitopes, a multi-epitope based subunit vaccine was computationally designed. Sequence-based analysis as well as structural characterization revealed the immunological effectiveness of this designed vaccine. Further molecular docking, molecular dynamics simulation and binding free energy estimation with Toll-like receptor 2 indicated strong structural associations of the vaccine with the immune receptor. The promising results are encouraging and need to be validated by additional wet laboratory studies for confirmation.Hamza Arshad DarSaba IsmailYasir WaheedSajjad AhmadZubia JamilHafsa AzizHelal F. HettaKhalid MuhammadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hamza Arshad Dar Saba Ismail Yasir Waheed Sajjad Ahmad Zubia Jamil Hafsa Aziz Helal F. Hetta Khalid Muhammad Designing a multi-epitope vaccine against Mycobacteroides abscessus by pangenome-reverse vaccinology
description	Abstract Mycobacteroides abscessus (Previously Mycobacterium abscessus) is an emerging microorganism of the newly defined genera Mycobacteroides that causes mainly skin and tissue diseases in humans. The recent availability of total 34 fully sequenced genomes of different strains belonging to this species has provided an opportunity to utilize this genomics data to gain novel insights and guide the development of specific antimicrobial therapies. In the present study, we collected collectively 34 complete genome sequences of M. abscessus from the NCBI GenBank database. Pangenome analysis was conducted on these genomes to understand the genetic diversity and to obtain proteins associated with its core genome. These core proteins were then subjected to various subtractive filters to identify potential antigenic targets that were subjected to multi-epitope vaccine design. Our analysis projected the open pangenome of M. abscessus containing 3443 core genes. After applying various stepwise filtration steps on the core proteins, a total of four potential antigenic targets were identified. Utilizing their constituent CD4 and CD8 T-cell epitopes, a multi-epitope based subunit vaccine was computationally designed. Sequence-based analysis as well as structural characterization revealed the immunological effectiveness of this designed vaccine. Further molecular docking, molecular dynamics simulation and binding free energy estimation with Toll-like receptor 2 indicated strong structural associations of the vaccine with the immune receptor. The promising results are encouraging and need to be validated by additional wet laboratory studies for confirmation.
format	article
author	Hamza Arshad Dar Saba Ismail Yasir Waheed Sajjad Ahmad Zubia Jamil Hafsa Aziz Helal F. Hetta Khalid Muhammad
author_facet	Hamza Arshad Dar Saba Ismail Yasir Waheed Sajjad Ahmad Zubia Jamil Hafsa Aziz Helal F. Hetta Khalid Muhammad
author_sort	Hamza Arshad Dar
title	Designing a multi-epitope vaccine against Mycobacteroides abscessus by pangenome-reverse vaccinology
title_short	Designing a multi-epitope vaccine against Mycobacteroides abscessus by pangenome-reverse vaccinology
title_full	Designing a multi-epitope vaccine against Mycobacteroides abscessus by pangenome-reverse vaccinology
title_fullStr	Designing a multi-epitope vaccine against Mycobacteroides abscessus by pangenome-reverse vaccinology
title_full_unstemmed	Designing a multi-epitope vaccine against Mycobacteroides abscessus by pangenome-reverse vaccinology
title_sort	designing a multi-epitope vaccine against mycobacteroides abscessus by pangenome-reverse vaccinology
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/173e4f38ae614439a7fe47c9cb2fba38
work_keys_str_mv	AT hamzaarshaddar designingamultiepitopevaccineagainstmycobacteroidesabscessusbypangenomereversevaccinology AT sabaismail designingamultiepitopevaccineagainstmycobacteroidesabscessusbypangenomereversevaccinology AT yasirwaheed designingamultiepitopevaccineagainstmycobacteroidesabscessusbypangenomereversevaccinology AT sajjadahmad designingamultiepitopevaccineagainstmycobacteroidesabscessusbypangenomereversevaccinology AT zubiajamil designingamultiepitopevaccineagainstmycobacteroidesabscessusbypangenomereversevaccinology AT hafsaaziz designingamultiepitopevaccineagainstmycobacteroidesabscessusbypangenomereversevaccinology AT helalfhetta designingamultiepitopevaccineagainstmycobacteroidesabscessusbypangenomereversevaccinology AT khalidmuhammad designingamultiepitopevaccineagainstmycobacteroidesabscessusbypangenomereversevaccinology
_version_	1718389494473818112

Designing a multi-epitope vaccine against Mycobacteroides abscessus by pangenome-reverse vaccinology

Ejemplares similares