The positive effect of chick embryo and nutrient mixture on bone marrow- derived mesenchymal stem cells from aging rats

Abstract The aging of many mammalian tissues is associated with loss of functional adult stem cells, especially bone marrow-derived mesenchymal stem cells (BMSCs). This study was aimed to analyze the biological effect of chick embryo (CE) and nutrient mixture (NM) on the BMSCs of aging rats. The agi...

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Autores principales:	Jia Ma, Yanru Guo, Jialei Hu, Yue Pan, Xia Qi, Huaxin Wang, Li Jia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/17451beead294da3977b277570435a05
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spelling	oai:doaj.org-article:17451beead294da3977b277570435a052021-12-02T11:41:14ZThe positive effect of chick embryo and nutrient mixture on bone marrow- derived mesenchymal stem cells from aging rats10.1038/s41598-018-25563-w2045-2322https://doaj.org/article/17451beead294da3977b277570435a052018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25563-whttps://doaj.org/toc/2045-2322Abstract The aging of many mammalian tissues is associated with loss of functional adult stem cells, especially bone marrow-derived mesenchymal stem cells (BMSCs). This study was aimed to analyze the biological effect of chick embryo (CE) and nutrient mixture (NM) on the BMSCs of aging rats. The aging rat model was established to be induced by D-galactose (500 mg/kg/d) for 90 days. Meanwhile, aging rats were fed with CE and NM in different dose manner by intragastric administration. At the end of the experimental period, serum was collected from rats and used for BMSCs culture. Flow cytometric analysis was used to investigate the BMSCs surface markers. Alizarin Red and oil red O staining were performed to evaluate the multi-lineage differentiation of BMSCs. The results showed that CE plus NM increased the telomere length of BMSCs and promoted BMSCs proliferation. Moreover, CE plus NM administration promoted BMSCs differentiation into osteoblasts and suppressed differentiation into adipocytes. High-throughput sequencing analysis revealed that there were 326 genes were up-regulated and 59 genes were down-regulated in BMSCs of aging rats treated with CE plus NM. In conclusion, CE plus NM supplement had potential to delay aging through the recovery of BMSCs senescence and could be used as a safe effective approach for nutritional therapy of anti-aging.Jia MaYanru GuoJialei HuYue PanXia QiHuaxin WangLi JiaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jia Ma Yanru Guo Jialei Hu Yue Pan Xia Qi Huaxin Wang Li Jia The positive effect of chick embryo and nutrient mixture on bone marrow- derived mesenchymal stem cells from aging rats
description	Abstract The aging of many mammalian tissues is associated with loss of functional adult stem cells, especially bone marrow-derived mesenchymal stem cells (BMSCs). This study was aimed to analyze the biological effect of chick embryo (CE) and nutrient mixture (NM) on the BMSCs of aging rats. The aging rat model was established to be induced by D-galactose (500 mg/kg/d) for 90 days. Meanwhile, aging rats were fed with CE and NM in different dose manner by intragastric administration. At the end of the experimental period, serum was collected from rats and used for BMSCs culture. Flow cytometric analysis was used to investigate the BMSCs surface markers. Alizarin Red and oil red O staining were performed to evaluate the multi-lineage differentiation of BMSCs. The results showed that CE plus NM increased the telomere length of BMSCs and promoted BMSCs proliferation. Moreover, CE plus NM administration promoted BMSCs differentiation into osteoblasts and suppressed differentiation into adipocytes. High-throughput sequencing analysis revealed that there were 326 genes were up-regulated and 59 genes were down-regulated in BMSCs of aging rats treated with CE plus NM. In conclusion, CE plus NM supplement had potential to delay aging through the recovery of BMSCs senescence and could be used as a safe effective approach for nutritional therapy of anti-aging.
format	article
author	Jia Ma Yanru Guo Jialei Hu Yue Pan Xia Qi Huaxin Wang Li Jia
author_facet	Jia Ma Yanru Guo Jialei Hu Yue Pan Xia Qi Huaxin Wang Li Jia
author_sort	Jia Ma
title	The positive effect of chick embryo and nutrient mixture on bone marrow- derived mesenchymal stem cells from aging rats
title_short	The positive effect of chick embryo and nutrient mixture on bone marrow- derived mesenchymal stem cells from aging rats
title_full	The positive effect of chick embryo and nutrient mixture on bone marrow- derived mesenchymal stem cells from aging rats
title_fullStr	The positive effect of chick embryo and nutrient mixture on bone marrow- derived mesenchymal stem cells from aging rats
title_full_unstemmed	The positive effect of chick embryo and nutrient mixture on bone marrow- derived mesenchymal stem cells from aging rats
title_sort	positive effect of chick embryo and nutrient mixture on bone marrow- derived mesenchymal stem cells from aging rats
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/17451beead294da3977b277570435a05
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The positive effect of chick embryo and nutrient mixture on bone marrow- derived mesenchymal stem cells from aging rats

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