Central Carbon Metabolism, Sodium-Motive Electron Transfer, and Ammonium Formation by the Vaginal Pathogen <i>Prevotella bivia</i>
Replacement of the <i>Lactobacillus</i> dominated vaginal microbiome by a mixed bacterial population including <i>Prevotella bivia</i> is associated with bacterial vaginosis (BV). To understand the impact of <i>P. bivia</i> on this microbiome, its growth requireme...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/174589f050bc489788c6e24cbb940c9b |
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| Sumario: | Replacement of the <i>Lactobacillus</i> dominated vaginal microbiome by a mixed bacterial population including <i>Prevotella bivia</i> is associated with bacterial vaginosis (BV). To understand the impact of <i>P. bivia</i> on this microbiome, its growth requirements and mode of energy production were studied. Anoxic growth with glucose depended on CO<sub>2</sub> and resulted in succinate formation, indicating phosphoenolpyruvate carboxylation and fumarate reduction as critical steps. The reductive branch of fermentation relied on two highly active, membrane-bound enzymes, namely the quinol:fumarate reductase (QFR) and Na<sup>+</sup>-translocating NADH:quinone oxidoreductase (NQR). Both enzymes were characterized by activity measurements, in-gel fluorography, and VIS difference spectroscopy, and the Na<sup>+</sup>-dependent build-up of a transmembrane voltage was demonstrated. NQR is a potential drug target for BV treatment since it is neither found in humans nor in <i>Lactobacillus</i>. In <i>P. bivia</i>, the highly active enzymes L-asparaginase and aspartate ammonia lyase catalyze the conversion of asparagine to the electron acceptor fumarate. However, the by-product ammonium is highly toxic. It has been proposed that <i>P. bivia</i> depends on ammonium-utilizing <i>Gardnerella vaginalis</i>, another typical pathogen associated with BV, and provides key nutrients to it. The product pattern of <i>P. bivia</i> growing on glucose in the presence of mixed amino acids substantiates this notion. |
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