[Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles

Meng-Yuan Shen,1,2,* Miao Wang,1,* Zhihua Liu,1 Shurong Wang,1 Ying Xie1 1Department of Endocrinology, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu, 215000, People’ s Republic of China; 2Department of Endocrinology, The First People’s Hospital of Fuyang Dis...

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Autores principales:	Shen MY, Wang M, Liu Z, Wang S, Xie Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	humanin apoptosis micrornas endothelial microparticles endothelial cells Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/174adbc16ee14ebe82a18108bf444928
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spelling	oai:doaj.org-article:174adbc16ee14ebe82a18108bf4449282021-12-02T14:37:10Z[Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles1178-7007https://doaj.org/article/174adbc16ee14ebe82a18108bf4449282021-05-01T00:00:00Zhttps://www.dovepress.com/gly14-humanin-ameliorates-high-glucose-induced-apoptosis-by-inhibiting-peer-reviewed-fulltext-article-DMSOhttps://doaj.org/toc/1178-7007Meng-Yuan Shen,1,2,* Miao Wang,1,* Zhihua Liu,1 Shurong Wang,1 Ying Xie1 1Department of Endocrinology, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu, 215000, People’ s Republic of China; 2Department of Endocrinology, The First People’s Hospital of Fuyang District of Hangzhou City, Hangzhou, Zhejiang, 310000, People’ s Republic of China*These authors contributed equally to this workCorrespondence: Ying XieDepartment of Endocrinology, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu, 215000, People’ s Republic of ChinaEmail 13013883877@126.comBackground: Humanin, a newly emerging endogenously expressed cytoprotective peptide, has been shown to have anti-apoptotic properties effects by protecting neuronal cells injury. Endothelial microparticles (EMPs) are considered as vital mediators in intercellular communication. EMPs may regulate various physiological and pathological processes by transferring mRNAs and microRNAs (miRNAs) to recipient cells.Methods: EMPs were isolated from human umbilical vein endothelial cells (HUVECs) by ultracentrifugation. EMPs were characterized by transmission electron microscopy and nanoparticle tracking analyses. Observation of EMPs uptake into HUVECs and the number of EMPs were realized by confocal microscopy. The expression of miR-155 was examined using real-time PCR. Cell apoptosis was examined by flow cytometry assay.Results: We found that high glucose (HG) increased the number of EMPs and upregulated the expression of miR-155 contained within EMPs, which was mitigated by HNG pretreatment. miR-155 overexpression in EMPs reversed the effects of HNG pretreatment and increased apoptosis of target cells. Effects of HNG pretreatment on HG-treated endothelial cells (ECs) were mitigated after miR-155 mimic transfection into HUVECs while were augmented after miR-155 inhibitor transfection into HUVECs.Conclusion: HNG inhibited HG-induced apoptosis of ECs and the effect of HNG may be mediated by inhibiting the transfer of EMPs miR-155 from HG-induced HUVECs to normal cells. This study provides a new direction for biological products related to humanin to treat vascular complications associated with all forms of diabetes mellitus.Keywords: humanin, apoptosis, microRNAs, endothelial microparticles, endothelial cellsShen MYWang MLiu ZWang SXie YDove Medical Pressarticlehumaninapoptosismicrornasendothelial microparticlesendothelial cellsSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 2335-2347 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	humanin apoptosis micrornas endothelial microparticles endothelial cells Specialties of internal medicine RC581-951
spellingShingle	humanin apoptosis micrornas endothelial microparticles endothelial cells Specialties of internal medicine RC581-951 Shen MY Wang M Liu Z Wang S Xie Y [Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles
description	Meng-Yuan Shen,1,2,* Miao Wang,1,* Zhihua Liu,1 Shurong Wang,1 Ying Xie1 1Department of Endocrinology, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu, 215000, People’ s Republic of China; 2Department of Endocrinology, The First People’s Hospital of Fuyang District of Hangzhou City, Hangzhou, Zhejiang, 310000, People’ s Republic of China*These authors contributed equally to this workCorrespondence: Ying XieDepartment of Endocrinology, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu, 215000, People’ s Republic of ChinaEmail 13013883877@126.comBackground: Humanin, a newly emerging endogenously expressed cytoprotective peptide, has been shown to have anti-apoptotic properties effects by protecting neuronal cells injury. Endothelial microparticles (EMPs) are considered as vital mediators in intercellular communication. EMPs may regulate various physiological and pathological processes by transferring mRNAs and microRNAs (miRNAs) to recipient cells.Methods: EMPs were isolated from human umbilical vein endothelial cells (HUVECs) by ultracentrifugation. EMPs were characterized by transmission electron microscopy and nanoparticle tracking analyses. Observation of EMPs uptake into HUVECs and the number of EMPs were realized by confocal microscopy. The expression of miR-155 was examined using real-time PCR. Cell apoptosis was examined by flow cytometry assay.Results: We found that high glucose (HG) increased the number of EMPs and upregulated the expression of miR-155 contained within EMPs, which was mitigated by HNG pretreatment. miR-155 overexpression in EMPs reversed the effects of HNG pretreatment and increased apoptosis of target cells. Effects of HNG pretreatment on HG-treated endothelial cells (ECs) were mitigated after miR-155 mimic transfection into HUVECs while were augmented after miR-155 inhibitor transfection into HUVECs.Conclusion: HNG inhibited HG-induced apoptosis of ECs and the effect of HNG may be mediated by inhibiting the transfer of EMPs miR-155 from HG-induced HUVECs to normal cells. This study provides a new direction for biological products related to humanin to treat vascular complications associated with all forms of diabetes mellitus.Keywords: humanin, apoptosis, microRNAs, endothelial microparticles, endothelial cells
format	article
author	Shen MY Wang M Liu Z Wang S Xie Y
author_facet	Shen MY Wang M Liu Z Wang S Xie Y
author_sort	Shen MY
title	[Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles
title_short	[Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles
title_full	[Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles
title_fullStr	[Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles
title_full_unstemmed	[Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles
title_sort	[gly14]-humanin ameliorates high glucose-induced apoptosis by inhibiting the expression of microrna-155 in endothelial microparticles
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/174adbc16ee14ebe82a18108bf444928
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_version_	1718390994507923456

[Gly14]-Humanin Ameliorates High Glucose-Induced Apoptosis by Inhibiting the Expression of MicroRNA-155 in Endothelial Microparticles

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