Identification of a B-Cell Epitope in the VP3 Protein of Senecavirus A

Senecavirus A (SVA) is a member of the genus <i>Senecavirus</i> of the family Picornaviridae. SVA-associated vesicular disease (SAVD) outbreaks have been extensively reported since 2014–2015. Characteristic symptoms include vesicular lesions on the snout and feet as well as lameness in a...

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Autores principales:	Mi Chen, Lulu Chen, Jing Wang, Chunxiao Mou, Zhenhai Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Senecavirus A VP3 protein B-cell epitope monoclonal antibody Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/174d021fea9543c0a2f8c485150969c4
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spelling	oai:doaj.org-article:174d021fea9543c0a2f8c485150969c42021-11-25T19:14:20ZIdentification of a B-Cell Epitope in the VP3 Protein of Senecavirus A10.3390/v131123001999-4915https://doaj.org/article/174d021fea9543c0a2f8c485150969c42021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2300https://doaj.org/toc/1999-4915Senecavirus A (SVA) is a member of the genus <i>Senecavirus</i> of the family Picornaviridae. SVA-associated vesicular disease (SAVD) outbreaks have been extensively reported since 2014–2015. Characteristic symptoms include vesicular lesions on the snout and feet as well as lameness in adult <i>pigs</i> and even death in <i>piglets</i>. The capsid protein VP3, a structural protein of SVA, is involved in viral replication and genome packaging. Here, we developed and characterized a <i>mouse</i> monoclonal antibody (mAb) 3E9 against VP3. A motif <sup>192</sup>GWFSLHKLTK<sup>201</sup> was identified as the linear B-cell epitope recognized by mAb 3E9 by using a panel of GFP-tagged epitope polypeptides. Sequence alignments show that <sup>192</sup>GWFSLHKLTK<sup>201</sup> was highly conserved in all SVA strains. Subsequently, alanine (A)-scanning mutagenesis indicated that W193, F194, L196, and H197 were the critical residues recognized by mAb 3E9. Further investigation with indirect immunofluorescence assay indicated that the VP3 protein was present in the cytoplasm during SVA replication. In addition, the mAb 3E9 specifically immunoprecipitated the VP3 protein from SVA-infected cells. Taken together, our results indicate that mAb 3E9 could be a powerful tool to work on the function of the VP3 protein during virus infection.Mi ChenLulu ChenJing WangChunxiao MouZhenhai ChenMDPI AGarticleSenecavirus AVP3 proteinB-cell epitopemonoclonal antibodyMicrobiologyQR1-502ENViruses, Vol 13, Iss 2300, p 2300 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Senecavirus A VP3 protein B-cell epitope monoclonal antibody Microbiology QR1-502
spellingShingle	Senecavirus A VP3 protein B-cell epitope monoclonal antibody Microbiology QR1-502 Mi Chen Lulu Chen Jing Wang Chunxiao Mou Zhenhai Chen Identification of a B-Cell Epitope in the VP3 Protein of Senecavirus A
description	Senecavirus A (SVA) is a member of the genus <i>Senecavirus</i> of the family Picornaviridae. SVA-associated vesicular disease (SAVD) outbreaks have been extensively reported since 2014–2015. Characteristic symptoms include vesicular lesions on the snout and feet as well as lameness in adult <i>pigs</i> and even death in <i>piglets</i>. The capsid protein VP3, a structural protein of SVA, is involved in viral replication and genome packaging. Here, we developed and characterized a <i>mouse</i> monoclonal antibody (mAb) 3E9 against VP3. A motif <sup>192</sup>GWFSLHKLTK<sup>201</sup> was identified as the linear B-cell epitope recognized by mAb 3E9 by using a panel of GFP-tagged epitope polypeptides. Sequence alignments show that <sup>192</sup>GWFSLHKLTK<sup>201</sup> was highly conserved in all SVA strains. Subsequently, alanine (A)-scanning mutagenesis indicated that W193, F194, L196, and H197 were the critical residues recognized by mAb 3E9. Further investigation with indirect immunofluorescence assay indicated that the VP3 protein was present in the cytoplasm during SVA replication. In addition, the mAb 3E9 specifically immunoprecipitated the VP3 protein from SVA-infected cells. Taken together, our results indicate that mAb 3E9 could be a powerful tool to work on the function of the VP3 protein during virus infection.
format	article
author	Mi Chen Lulu Chen Jing Wang Chunxiao Mou Zhenhai Chen
author_facet	Mi Chen Lulu Chen Jing Wang Chunxiao Mou Zhenhai Chen
author_sort	Mi Chen
title	Identification of a B-Cell Epitope in the VP3 Protein of Senecavirus A
title_short	Identification of a B-Cell Epitope in the VP3 Protein of Senecavirus A
title_full	Identification of a B-Cell Epitope in the VP3 Protein of Senecavirus A
title_fullStr	Identification of a B-Cell Epitope in the VP3 Protein of Senecavirus A
title_full_unstemmed	Identification of a B-Cell Epitope in the VP3 Protein of Senecavirus A
title_sort	identification of a b-cell epitope in the vp3 protein of senecavirus a
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/174d021fea9543c0a2f8c485150969c4
work_keys_str_mv	AT michen identificationofabcellepitopeinthevp3proteinofsenecavirusa AT luluchen identificationofabcellepitopeinthevp3proteinofsenecavirusa AT jingwang identificationofabcellepitopeinthevp3proteinofsenecavirusa AT chunxiaomou identificationofabcellepitopeinthevp3proteinofsenecavirusa AT zhenhaichen identificationofabcellepitopeinthevp3proteinofsenecavirusa
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Identification of a B-Cell Epitope in the VP3 Protein of Senecavirus A

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