Dendritic cells play no significant role in the laser-induced choroidal neovascularization model

Abstract Age-related macular degeneration (AMD) is genetically associated with complement. Dendritic cells (DCs) play key roles during innate and adaptive immunity, and express complement components and their receptors. We investigated ocular DC heterogeneity and the role of DCs in the laser-induced...

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Autores principales:	Steven Droho, Harris Perlman, Jeremy A. Lavine
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/174d31a7125748e59e5310094d20e196
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spelling	oai:doaj.org-article:174d31a7125748e59e5310094d20e1962021-12-02T18:53:14ZDendritic cells play no significant role in the laser-induced choroidal neovascularization model10.1038/s41598-021-96704-x2045-2322https://doaj.org/article/174d31a7125748e59e5310094d20e1962021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96704-xhttps://doaj.org/toc/2045-2322Abstract Age-related macular degeneration (AMD) is genetically associated with complement. Dendritic cells (DCs) play key roles during innate and adaptive immunity, and express complement components and their receptors. We investigated ocular DC heterogeneity and the role of DCs in the laser-induced choroidal neovascularization (CNV) model. In order to determine the function of DCs, we used two models of DC deficiency: the Flt3 −/− and Flt3l −/− mouse. We identified three types of ocular DCs: plasmacytoid DC, classical DC-1, and classical DC-2. At steady-state, classical DCs were found in the iris and choroid but were not detectable in the retina. Plasmacytoid DCs existed at very low levels in iris, choroid, and retina. After laser injury, the number of each DC subset was up-regulated in the choroid and retina. In Flt3 −/− mice, we found reduced numbers of classical DCs at steady-state, but each DC subset equally increased after laser injury between wildtype and Flt3 −/− mice. In Flt3l −/− mice, each DC subsets was severely reduced after laser injury. Neither Flt3 −/− or Flt3l −/− mice demonstrated reduced CNV area compared to wildtype mice. DCs do not play any significant role during the laser-induced CNV model of neovascular AMD.Steven DrohoHarris PerlmanJeremy A. LavineNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Steven Droho Harris Perlman Jeremy A. Lavine Dendritic cells play no significant role in the laser-induced choroidal neovascularization model
description	Abstract Age-related macular degeneration (AMD) is genetically associated with complement. Dendritic cells (DCs) play key roles during innate and adaptive immunity, and express complement components and their receptors. We investigated ocular DC heterogeneity and the role of DCs in the laser-induced choroidal neovascularization (CNV) model. In order to determine the function of DCs, we used two models of DC deficiency: the Flt3 −/− and Flt3l −/− mouse. We identified three types of ocular DCs: plasmacytoid DC, classical DC-1, and classical DC-2. At steady-state, classical DCs were found in the iris and choroid but were not detectable in the retina. Plasmacytoid DCs existed at very low levels in iris, choroid, and retina. After laser injury, the number of each DC subset was up-regulated in the choroid and retina. In Flt3 −/− mice, we found reduced numbers of classical DCs at steady-state, but each DC subset equally increased after laser injury between wildtype and Flt3 −/− mice. In Flt3l −/− mice, each DC subsets was severely reduced after laser injury. Neither Flt3 −/− or Flt3l −/− mice demonstrated reduced CNV area compared to wildtype mice. DCs do not play any significant role during the laser-induced CNV model of neovascular AMD.
format	article
author	Steven Droho Harris Perlman Jeremy A. Lavine
author_facet	Steven Droho Harris Perlman Jeremy A. Lavine
author_sort	Steven Droho
title	Dendritic cells play no significant role in the laser-induced choroidal neovascularization model
title_short	Dendritic cells play no significant role in the laser-induced choroidal neovascularization model
title_full	Dendritic cells play no significant role in the laser-induced choroidal neovascularization model
title_fullStr	Dendritic cells play no significant role in the laser-induced choroidal neovascularization model
title_full_unstemmed	Dendritic cells play no significant role in the laser-induced choroidal neovascularization model
title_sort	dendritic cells play no significant role in the laser-induced choroidal neovascularization model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/174d31a7125748e59e5310094d20e196
work_keys_str_mv	AT stevendroho dendriticcellsplaynosignificantroleinthelaserinducedchoroidalneovascularizationmodel AT harrisperlman dendriticcellsplaynosignificantroleinthelaserinducedchoroidalneovascularizationmodel AT jeremyalavine dendriticcellsplaynosignificantroleinthelaserinducedchoroidalneovascularizationmodel
_version_	1718377311089197056

Dendritic cells play no significant role in the laser-induced choroidal neovascularization model

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