Infecting mosquitoes alters DENV-2 characteristics and enhances hemorrhage-induction potential in Stat1-/- mice.

Dengue is one of the most prevalent arthropod-borne viral diseases in humans. There is still no effective vaccine or treatment to date. Previous studies showed that mosquito-derived factors present in saliva or salivary gland extract (SGE) contribute to the pathogenesis of dengue. In this study, we...

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Autores principales:	Ka Wan Cheang, Wen-Yu Chen, Betty A Wu-Hsieh, Shin-Hong Shiao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/175c2bbd6c2d4001a2d5ed2d4126dd95
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spelling	oai:doaj.org-article:175c2bbd6c2d4001a2d5ed2d4126dd952021-12-02T20:24:15ZInfecting mosquitoes alters DENV-2 characteristics and enhances hemorrhage-induction potential in Stat1-/- mice.1935-27271935-273510.1371/journal.pntd.0009728https://doaj.org/article/175c2bbd6c2d4001a2d5ed2d4126dd952021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009728https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Dengue is one of the most prevalent arthropod-borne viral diseases in humans. There is still no effective vaccine or treatment to date. Previous studies showed that mosquito-derived factors present in saliva or salivary gland extract (SGE) contribute to the pathogenesis of dengue. In this study, we aimed to investigate the interplay between mosquito vector and DENV and to address the question of whether the mosquito vector alters the virus that leads to consequential disease manifestations in the mammalian host. DENV2 cultured in C6/36 cell line (culture-DENV2) was injected to Aedes aegypti intrathoracically. Saliva was collected from infected mosquitoes 7 days later. Exploiting the sensitivity of Stat1-/- mice to low dose of DENV2 delivered intradermally, we showed that DENV2 collected in infected mosquito saliva (msq-DENV2) induced more severe hemorrhage in mice than their culture counterpart. Msq-DENV2 was characterized by smaller particle size, larger plaque size and more rapid growth in mosquito as well as mammalian cell lines compared to culture-DENV2. In addition, msq-DENV2 was more efficient than culture-DENV2 in inducing Tnf mRNA production by mouse macrophage. Together, our results point to the possibility that the mosquito vector provides an environment that alters DENV2 by changing its growth characteristics as well as its potential to cause disease.Ka Wan CheangWen-Yu ChenBetty A Wu-HsiehShin-Hong ShiaoPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 8, p e0009728 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ka Wan Cheang Wen-Yu Chen Betty A Wu-Hsieh Shin-Hong Shiao Infecting mosquitoes alters DENV-2 characteristics and enhances hemorrhage-induction potential in Stat1-/- mice.
description	Dengue is one of the most prevalent arthropod-borne viral diseases in humans. There is still no effective vaccine or treatment to date. Previous studies showed that mosquito-derived factors present in saliva or salivary gland extract (SGE) contribute to the pathogenesis of dengue. In this study, we aimed to investigate the interplay between mosquito vector and DENV and to address the question of whether the mosquito vector alters the virus that leads to consequential disease manifestations in the mammalian host. DENV2 cultured in C6/36 cell line (culture-DENV2) was injected to Aedes aegypti intrathoracically. Saliva was collected from infected mosquitoes 7 days later. Exploiting the sensitivity of Stat1-/- mice to low dose of DENV2 delivered intradermally, we showed that DENV2 collected in infected mosquito saliva (msq-DENV2) induced more severe hemorrhage in mice than their culture counterpart. Msq-DENV2 was characterized by smaller particle size, larger plaque size and more rapid growth in mosquito as well as mammalian cell lines compared to culture-DENV2. In addition, msq-DENV2 was more efficient than culture-DENV2 in inducing Tnf mRNA production by mouse macrophage. Together, our results point to the possibility that the mosquito vector provides an environment that alters DENV2 by changing its growth characteristics as well as its potential to cause disease.
format	article
author	Ka Wan Cheang Wen-Yu Chen Betty A Wu-Hsieh Shin-Hong Shiao
author_facet	Ka Wan Cheang Wen-Yu Chen Betty A Wu-Hsieh Shin-Hong Shiao
author_sort	Ka Wan Cheang
title	Infecting mosquitoes alters DENV-2 characteristics and enhances hemorrhage-induction potential in Stat1-/- mice.
title_short	Infecting mosquitoes alters DENV-2 characteristics and enhances hemorrhage-induction potential in Stat1-/- mice.
title_full	Infecting mosquitoes alters DENV-2 characteristics and enhances hemorrhage-induction potential in Stat1-/- mice.
title_fullStr	Infecting mosquitoes alters DENV-2 characteristics and enhances hemorrhage-induction potential in Stat1-/- mice.
title_full_unstemmed	Infecting mosquitoes alters DENV-2 characteristics and enhances hemorrhage-induction potential in Stat1-/- mice.
title_sort	infecting mosquitoes alters denv-2 characteristics and enhances hemorrhage-induction potential in stat1-/- mice.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/175c2bbd6c2d4001a2d5ed2d4126dd95
work_keys_str_mv	AT kawancheang infectingmosquitoesaltersdenv2characteristicsandenhanceshemorrhageinductionpotentialinstat1mice AT wenyuchen infectingmosquitoesaltersdenv2characteristicsandenhanceshemorrhageinductionpotentialinstat1mice AT bettyawuhsieh infectingmosquitoesaltersdenv2characteristicsandenhanceshemorrhageinductionpotentialinstat1mice AT shinhongshiao infectingmosquitoesaltersdenv2characteristicsandenhanceshemorrhageinductionpotentialinstat1mice
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Infecting mosquitoes alters DENV-2 characteristics and enhances hemorrhage-induction potential in Stat1-/- mice.

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