Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis

Abstract Cnidarians are emerging model organisms for cell and molecular biology research. However, successful cell culture development has been challenging due to incomplete tissue dissociation and contamination. In this report, we developed and tested several different methodologies to culture prim...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	James D. Nowotny, Michael T. Connelly, Nikki Traylor-Knowles
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/175cdaa83688460c8babaf0723585c9c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:175cdaa83688460c8babaf0723585c9c
record_format	dspace
spelling	oai:doaj.org-article:175cdaa83688460c8babaf0723585c9c2021-12-02T10:54:06ZNovel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis10.1038/s41598-021-83549-72045-2322https://doaj.org/article/175cdaa83688460c8babaf0723585c9c2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83549-7https://doaj.org/toc/2045-2322Abstract Cnidarians are emerging model organisms for cell and molecular biology research. However, successful cell culture development has been challenging due to incomplete tissue dissociation and contamination. In this report, we developed and tested several different methodologies to culture primary cells from all tissues of two species of Cnidaria: Nematostella vectensis and Pocillopora damicornis. In over 170 replicated cell cultures, we demonstrate that physical dissociation was the most successful method for viable and diverse N. vectensis cells while antibiotic-assisted dissociation was most successful for viable and diverse P. damicornis cells. We also demonstrate that a rigorous antibiotic pretreatment results in less initial contamination in cell cultures. Primary cultures of both species averaged 12–13 days of viability, showed proliferation, and maintained high cell diversity including cnidocytes, nematosomes, putative gastrodermal, and epidermal cells. Overall, this work will contribute a needed tool for furthering functional cell biology experiments in Cnidaria.James D. NowotnyMichael T. ConnellyNikki Traylor-KnowlesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q James D. Nowotny Michael T. Connelly Nikki Traylor-Knowles Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis
description	Abstract Cnidarians are emerging model organisms for cell and molecular biology research. However, successful cell culture development has been challenging due to incomplete tissue dissociation and contamination. In this report, we developed and tested several different methodologies to culture primary cells from all tissues of two species of Cnidaria: Nematostella vectensis and Pocillopora damicornis. In over 170 replicated cell cultures, we demonstrate that physical dissociation was the most successful method for viable and diverse N. vectensis cells while antibiotic-assisted dissociation was most successful for viable and diverse P. damicornis cells. We also demonstrate that a rigorous antibiotic pretreatment results in less initial contamination in cell cultures. Primary cultures of both species averaged 12–13 days of viability, showed proliferation, and maintained high cell diversity including cnidocytes, nematosomes, putative gastrodermal, and epidermal cells. Overall, this work will contribute a needed tool for furthering functional cell biology experiments in Cnidaria.
format	article
author	James D. Nowotny Michael T. Connelly Nikki Traylor-Knowles
author_facet	James D. Nowotny Michael T. Connelly Nikki Traylor-Knowles
author_sort	James D. Nowotny
title	Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis
title_short	Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis
title_full	Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis
title_fullStr	Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis
title_full_unstemmed	Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis
title_sort	novel methods to establish whole-body primary cell cultures for the cnidarians nematostella vectensis and pocillopora damicornis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/175cdaa83688460c8babaf0723585c9c
work_keys_str_mv	AT jamesdnowotny novelmethodstoestablishwholebodyprimarycellculturesforthecnidariansnematostellavectensisandpocilloporadamicornis AT michaeltconnelly novelmethodstoestablishwholebodyprimarycellculturesforthecnidariansnematostellavectensisandpocilloporadamicornis AT nikkitraylorknowles novelmethodstoestablishwholebodyprimarycellculturesforthecnidariansnematostellavectensisandpocilloporadamicornis
_version_	1718396454356123648

Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis

Ejemplares similares