Analysis of human and mouse reprogramming of somatic cells to induced pluripotent stem cells. What is in the plate?

After the hope and controversy brought by embryonic stem cells two decades ago for regenerative medicine, a new turn has been taken in pluripotent cells research when, in 2006, Yamanaka's group reported the reprogramming of fibroblasts to pluripotent cells with the transfection of only four tra...

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Autores principales:	Stéphanie Boué, Ida Paramonov, María José Barrero, Juan Carlos Izpisúa Belmonte
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/17601a68ea8946ac9c4e1c2bffe68a5c
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spelling	oai:doaj.org-article:17601a68ea8946ac9c4e1c2bffe68a5c2021-11-18T06:35:05ZAnalysis of human and mouse reprogramming of somatic cells to induced pluripotent stem cells. What is in the plate?1932-620310.1371/journal.pone.0012664https://doaj.org/article/17601a68ea8946ac9c4e1c2bffe68a5c2010-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20862250/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203After the hope and controversy brought by embryonic stem cells two decades ago for regenerative medicine, a new turn has been taken in pluripotent cells research when, in 2006, Yamanaka's group reported the reprogramming of fibroblasts to pluripotent cells with the transfection of only four transcription factors. Since then many researchers have managed to reprogram somatic cells from diverse origins into pluripotent cells, though the cellular and genetic consequences of reprogramming remain largely unknown. Furthermore, it is still unclear whether induced pluripotent stem cells (iPSCs) are truly functionally equivalent to embryonic stem cells (ESCs) and if they demonstrate the same differentiation potential as ESCs. There are a large number of reprogramming experiments published so far encompassing genome-wide transcriptional profiling of the cells of origin, the iPSCs and ESCs, which are used as standards of pluripotent cells and allow us to provide here an in-depth analysis of transcriptional profiles of human and mouse cells before and after reprogramming. When compared to ESCs, iPSCs, as expected, share a common pluripotency/self-renewal network. Perhaps more importantly, they also show differences in the expression of some genes. We concentrated our efforts on the study of bivalent domain-containing genes (in ESCs) which are not expressed in ESCs, as they are supposedly important for differentiation and should possess a poised status in pluripotent cells, i.e. be ready to but not yet be expressed. We studied each iPSC line separately to estimate the quality of the reprogramming and saw a correlation of the lowest number of such genes expressed in each respective iPSC line with the stringency of the pluripotency test achieved by the line. We propose that the study of expression of bivalent domain-containing genes, which are normally silenced in ESCs, gives a valuable indication of the quality of the iPSC line, and could be used to select the best iPSC lines out of a large number of lines generated in each reprogramming experiment.Stéphanie BouéIda ParamonovMaría José BarreroJuan Carlos Izpisúa BelmontePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 9 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stéphanie Boué Ida Paramonov María José Barrero Juan Carlos Izpisúa Belmonte Analysis of human and mouse reprogramming of somatic cells to induced pluripotent stem cells. What is in the plate?
description	After the hope and controversy brought by embryonic stem cells two decades ago for regenerative medicine, a new turn has been taken in pluripotent cells research when, in 2006, Yamanaka's group reported the reprogramming of fibroblasts to pluripotent cells with the transfection of only four transcription factors. Since then many researchers have managed to reprogram somatic cells from diverse origins into pluripotent cells, though the cellular and genetic consequences of reprogramming remain largely unknown. Furthermore, it is still unclear whether induced pluripotent stem cells (iPSCs) are truly functionally equivalent to embryonic stem cells (ESCs) and if they demonstrate the same differentiation potential as ESCs. There are a large number of reprogramming experiments published so far encompassing genome-wide transcriptional profiling of the cells of origin, the iPSCs and ESCs, which are used as standards of pluripotent cells and allow us to provide here an in-depth analysis of transcriptional profiles of human and mouse cells before and after reprogramming. When compared to ESCs, iPSCs, as expected, share a common pluripotency/self-renewal network. Perhaps more importantly, they also show differences in the expression of some genes. We concentrated our efforts on the study of bivalent domain-containing genes (in ESCs) which are not expressed in ESCs, as they are supposedly important for differentiation and should possess a poised status in pluripotent cells, i.e. be ready to but not yet be expressed. We studied each iPSC line separately to estimate the quality of the reprogramming and saw a correlation of the lowest number of such genes expressed in each respective iPSC line with the stringency of the pluripotency test achieved by the line. We propose that the study of expression of bivalent domain-containing genes, which are normally silenced in ESCs, gives a valuable indication of the quality of the iPSC line, and could be used to select the best iPSC lines out of a large number of lines generated in each reprogramming experiment.
format	article
author	Stéphanie Boué Ida Paramonov María José Barrero Juan Carlos Izpisúa Belmonte
author_facet	Stéphanie Boué Ida Paramonov María José Barrero Juan Carlos Izpisúa Belmonte
author_sort	Stéphanie Boué
title	Analysis of human and mouse reprogramming of somatic cells to induced pluripotent stem cells. What is in the plate?
title_short	Analysis of human and mouse reprogramming of somatic cells to induced pluripotent stem cells. What is in the plate?
title_full	Analysis of human and mouse reprogramming of somatic cells to induced pluripotent stem cells. What is in the plate?
title_fullStr	Analysis of human and mouse reprogramming of somatic cells to induced pluripotent stem cells. What is in the plate?
title_full_unstemmed	Analysis of human and mouse reprogramming of somatic cells to induced pluripotent stem cells. What is in the plate?
title_sort	analysis of human and mouse reprogramming of somatic cells to induced pluripotent stem cells. what is in the plate?
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/17601a68ea8946ac9c4e1c2bffe68a5c
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Analysis of human and mouse reprogramming of somatic cells to induced pluripotent stem cells. What is in the plate?

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