Evaluation of anti-inflammatory and immunomodulatory activity of Chyawanprash on particulate matter-induced pulmonary disease in mice

Background: Particulate matter (PM) is the major component of air pollution, which includes emissions from both anthropogenic and natural sources. PM, with aerodynamic diameter of 2.5 ± 10 μm can remain in the air for a long time and be deposited in the lungs through inhalation and hence, is a major...

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Autores principales:	Satyendra Kumar, Padmanabha Rugvedi, Kamaraj Mani, Arun Gupta
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	BALF Chyawanprash Cytokines PM2.5 Pulmonary disease Miscellaneous systems and treatments RZ409.7-999
Acceso en línea:	https://doaj.org/article/1760c77605d84901a711404450702201
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spelling	oai:doaj.org-article:1760c77605d84901a7114044507022012021-12-02T04:59:21ZEvaluation of anti-inflammatory and immunomodulatory activity of Chyawanprash on particulate matter-induced pulmonary disease in mice0975-947610.1016/j.jaim.2021.06.022https://doaj.org/article/1760c77605d84901a7114044507022012021-10-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0975947621001303https://doaj.org/toc/0975-9476Background: Particulate matter (PM) is the major component of air pollution, which includes emissions from both anthropogenic and natural sources. PM, with aerodynamic diameter of 2.5 ± 10 μm can remain in the air for a long time and be deposited in the lungs through inhalation and hence, is a major threat to human health. Objective: The objective of the present study was to examine the protective effect of Chyawanprash (CP) on PM-induced pulmonary disease through estimation of cytokines and immunoglobulins. Materials and methods: CP, standard drug, and vehicle (Group G1 to Group G7) were administered orally at the dose volume of 10 ml/kg, for 28 consecutive days (Prophylactic treatment; i.e., Day 1 to Day 28) and next 10 days (i.e., Day 29 to Day 38) of co-treatment with inducing agent PM2.5 intratracheally. Animals of group G6 (Inhalation + control) and G7 (Inhalation + CP) were exposed group-wise to PM2.5 aerosol (2 mg/5 ml, 15 min) via inhalation in histamine chamber on Days 29, 31, 33, 35, and 37. On Day 38, animals were anesthetised and blood and broncho alveolar lavage fluid (BALF) were collected. Animals were sacrificed and lungs were collected for histology. Results: Prophylactic benefit of CP against pulmonary pathology was evidenced by the inhibition of inflammatory cytokines (BALF: TNF a, IFN-g, IL-7, IL-6 and lung: TNFa, Histamine and IL-6), chemokines (Lung: MMP-9), inflammatory cell infiltration (cell counts in BALF), and histopatholoy in experimental mice model. Conclusion: These findings suggest that CP has potential benefit in protecting from harmful effects caused by air pollutants such as PM2.5.Satyendra KumarPadmanabha RugvediKamaraj ManiArun GuptaElsevierarticleBALFChyawanprashCytokinesPM2.5Pulmonary diseaseMiscellaneous systems and treatmentsRZ409.7-999ENJournal of Ayurveda and Integrative Medicine, Vol 12, Iss 4, Pp 649-656 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	BALF Chyawanprash Cytokines PM2.5 Pulmonary disease Miscellaneous systems and treatments RZ409.7-999
spellingShingle	BALF Chyawanprash Cytokines PM2.5 Pulmonary disease Miscellaneous systems and treatments RZ409.7-999 Satyendra Kumar Padmanabha Rugvedi Kamaraj Mani Arun Gupta Evaluation of anti-inflammatory and immunomodulatory activity of Chyawanprash on particulate matter-induced pulmonary disease in mice
description	Background: Particulate matter (PM) is the major component of air pollution, which includes emissions from both anthropogenic and natural sources. PM, with aerodynamic diameter of 2.5 ± 10 μm can remain in the air for a long time and be deposited in the lungs through inhalation and hence, is a major threat to human health. Objective: The objective of the present study was to examine the protective effect of Chyawanprash (CP) on PM-induced pulmonary disease through estimation of cytokines and immunoglobulins. Materials and methods: CP, standard drug, and vehicle (Group G1 to Group G7) were administered orally at the dose volume of 10 ml/kg, for 28 consecutive days (Prophylactic treatment; i.e., Day 1 to Day 28) and next 10 days (i.e., Day 29 to Day 38) of co-treatment with inducing agent PM2.5 intratracheally. Animals of group G6 (Inhalation + control) and G7 (Inhalation + CP) were exposed group-wise to PM2.5 aerosol (2 mg/5 ml, 15 min) via inhalation in histamine chamber on Days 29, 31, 33, 35, and 37. On Day 38, animals were anesthetised and blood and broncho alveolar lavage fluid (BALF) were collected. Animals were sacrificed and lungs were collected for histology. Results: Prophylactic benefit of CP against pulmonary pathology was evidenced by the inhibition of inflammatory cytokines (BALF: TNF a, IFN-g, IL-7, IL-6 and lung: TNFa, Histamine and IL-6), chemokines (Lung: MMP-9), inflammatory cell infiltration (cell counts in BALF), and histopatholoy in experimental mice model. Conclusion: These findings suggest that CP has potential benefit in protecting from harmful effects caused by air pollutants such as PM2.5.
format	article
author	Satyendra Kumar Padmanabha Rugvedi Kamaraj Mani Arun Gupta
author_facet	Satyendra Kumar Padmanabha Rugvedi Kamaraj Mani Arun Gupta
author_sort	Satyendra Kumar
title	Evaluation of anti-inflammatory and immunomodulatory activity of Chyawanprash on particulate matter-induced pulmonary disease in mice
title_short	Evaluation of anti-inflammatory and immunomodulatory activity of Chyawanprash on particulate matter-induced pulmonary disease in mice
title_full	Evaluation of anti-inflammatory and immunomodulatory activity of Chyawanprash on particulate matter-induced pulmonary disease in mice
title_fullStr	Evaluation of anti-inflammatory and immunomodulatory activity of Chyawanprash on particulate matter-induced pulmonary disease in mice
title_full_unstemmed	Evaluation of anti-inflammatory and immunomodulatory activity of Chyawanprash on particulate matter-induced pulmonary disease in mice
title_sort	evaluation of anti-inflammatory and immunomodulatory activity of chyawanprash on particulate matter-induced pulmonary disease in mice
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/1760c77605d84901a711404450702201
work_keys_str_mv	AT satyendrakumar evaluationofantiinflammatoryandimmunomodulatoryactivityofchyawanprashonparticulatematterinducedpulmonarydiseaseinmice AT padmanabharugvedi evaluationofantiinflammatoryandimmunomodulatoryactivityofchyawanprashonparticulatematterinducedpulmonarydiseaseinmice AT kamarajmani evaluationofantiinflammatoryandimmunomodulatoryactivityofchyawanprashonparticulatematterinducedpulmonarydiseaseinmice AT arungupta evaluationofantiinflammatoryandimmunomodulatoryactivityofchyawanprashonparticulatematterinducedpulmonarydiseaseinmice
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Evaluation of anti-inflammatory and immunomodulatory activity of Chyawanprash on particulate matter-induced pulmonary disease in mice

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