Sonic Hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation.

The genetic basis and mechanisms behind the morphological variation observed throughout the animal kingdom is still relatively unknown. In the present work we have focused on the establishment of the chicken comb-morphology by exploring the Pea-comb mutant. The wild-type single-comb is reduced in si...

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Autores principales:	Henrik Boije, Mohammad Harun-Or-Rashid, Yu-Jen Lee, Freyja Imsland, Nicolas Bruneau, Agathe Vieaud, David Gourichon, Michèle Tixier-Boichard, Bertrand Bed'hom, Leif Andersson, Finn Hallböök
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1760f5ff95ac4c218b472c3d68fa7ddd
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spelling	oai:doaj.org-article:1760f5ff95ac4c218b472c3d68fa7ddd2021-11-18T08:06:16ZSonic Hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation.1932-620310.1371/journal.pone.0050890https://doaj.org/article/1760f5ff95ac4c218b472c3d68fa7ddd2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23227218/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The genetic basis and mechanisms behind the morphological variation observed throughout the animal kingdom is still relatively unknown. In the present work we have focused on the establishment of the chicken comb-morphology by exploring the Pea-comb mutant. The wild-type single-comb is reduced in size and distorted in the Pea-comb mutant. Pea-comb is formed by a lateral expansion of the central comb anlage into three ridges and is caused by a mutation in SOX5, which induces ectopic expression of the SOX5 transcription factor in mesenchyme under the developing comb. Analysis of differential gene expression identified decreased Sonic hedgehog (SHH) receptor expression in Pea-comb mesenchyme. By experimentally blocking SHH with cyclopamine, the wild-type single-comb was transformed into a Pea-comb-like phenotype. The results show that the patterning of the chicken comb is under the control of SHH and suggest that ectopic SOX5 expression in the Pea-comb change the response of mesenchyme to SHH signalling with altered comb morphogenesis as a result. A role for the mesenchyme during comb morphogenesis is further supported by the recent finding that another comb-mutant (Rose-comb), is caused by ectopic expression of a transcription factor in comb mesenchyme. The present study does not only give knowledge about how the chicken comb is formed, it also adds to our understanding how mutations or genetic polymorphisms may contribute to inherited variations in the human face.Henrik BoijeMohammad Harun-Or-RashidYu-Jen LeeFreyja ImslandNicolas BruneauAgathe VieaudDavid GourichonMichèle Tixier-BoichardBertrand Bed'homLeif AnderssonFinn HallböökPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e50890 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Henrik Boije Mohammad Harun-Or-Rashid Yu-Jen Lee Freyja Imsland Nicolas Bruneau Agathe Vieaud David Gourichon Michèle Tixier-Boichard Bertrand Bed'hom Leif Andersson Finn Hallböök Sonic Hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation.
description	The genetic basis and mechanisms behind the morphological variation observed throughout the animal kingdom is still relatively unknown. In the present work we have focused on the establishment of the chicken comb-morphology by exploring the Pea-comb mutant. The wild-type single-comb is reduced in size and distorted in the Pea-comb mutant. Pea-comb is formed by a lateral expansion of the central comb anlage into three ridges and is caused by a mutation in SOX5, which induces ectopic expression of the SOX5 transcription factor in mesenchyme under the developing comb. Analysis of differential gene expression identified decreased Sonic hedgehog (SHH) receptor expression in Pea-comb mesenchyme. By experimentally blocking SHH with cyclopamine, the wild-type single-comb was transformed into a Pea-comb-like phenotype. The results show that the patterning of the chicken comb is under the control of SHH and suggest that ectopic SOX5 expression in the Pea-comb change the response of mesenchyme to SHH signalling with altered comb morphogenesis as a result. A role for the mesenchyme during comb morphogenesis is further supported by the recent finding that another comb-mutant (Rose-comb), is caused by ectopic expression of a transcription factor in comb mesenchyme. The present study does not only give knowledge about how the chicken comb is formed, it also adds to our understanding how mutations or genetic polymorphisms may contribute to inherited variations in the human face.
format	article
author	Henrik Boije Mohammad Harun-Or-Rashid Yu-Jen Lee Freyja Imsland Nicolas Bruneau Agathe Vieaud David Gourichon Michèle Tixier-Boichard Bertrand Bed'hom Leif Andersson Finn Hallböök
author_facet	Henrik Boije Mohammad Harun-Or-Rashid Yu-Jen Lee Freyja Imsland Nicolas Bruneau Agathe Vieaud David Gourichon Michèle Tixier-Boichard Bertrand Bed'hom Leif Andersson Finn Hallböök
author_sort	Henrik Boije
title	Sonic Hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation.
title_short	Sonic Hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation.
title_full	Sonic Hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation.
title_fullStr	Sonic Hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation.
title_full_unstemmed	Sonic Hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation.
title_sort	sonic hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1760f5ff95ac4c218b472c3d68fa7ddd
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Sonic Hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation.

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