Clog-free high-throughput microfluidic cell isolation with multifunctional microposts

Abstract Microfluidics have been applied to filtration of rare tumor cells from the blood as liquid biopsies. Processing is highly limited by low flow rates and device clogging due to a single function of fluidic paths. A novel method using multifunctional hybrid functional microposts was developed....

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Autores principales:	Dilip Venugopal, Nanda Kasani, Yariswamy Manjunath, Guangfu Li, Jussuf T. Kaifi, Jae W. Kwon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/17765ab8ef0a4ba5a09651028283e7a5
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spelling	oai:doaj.org-article:17765ab8ef0a4ba5a09651028283e7a52021-12-02T18:51:46ZClog-free high-throughput microfluidic cell isolation with multifunctional microposts10.1038/s41598-021-94123-62045-2322https://doaj.org/article/17765ab8ef0a4ba5a09651028283e7a52021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94123-6https://doaj.org/toc/2045-2322Abstract Microfluidics have been applied to filtration of rare tumor cells from the blood as liquid biopsies. Processing is highly limited by low flow rates and device clogging due to a single function of fluidic paths. A novel method using multifunctional hybrid functional microposts was developed. A swift by-passing route for non-tumor cells was integrated to prevent very common clogging problems. Performance was characterized using microbeads (10 µm) and human cancer cells that were spiked in human blood. Design-I showed a capture efficiency of 96% for microbeads and 87% for cancer cells at 1 ml/min flow rate. An improved Design-II presented a higher capture efficiency of 100% for microbeads and 96% for cancer cells. Our method of utilizing various microfluidic functions of separation, bypass and capture has successfully guaranteed highly efficient separation of rare cells from biological fluids.Dilip VenugopalNanda KasaniYariswamy ManjunathGuangfu LiJussuf T. KaifiJae W. KwonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dilip Venugopal Nanda Kasani Yariswamy Manjunath Guangfu Li Jussuf T. Kaifi Jae W. Kwon Clog-free high-throughput microfluidic cell isolation with multifunctional microposts
description	Abstract Microfluidics have been applied to filtration of rare tumor cells from the blood as liquid biopsies. Processing is highly limited by low flow rates and device clogging due to a single function of fluidic paths. A novel method using multifunctional hybrid functional microposts was developed. A swift by-passing route for non-tumor cells was integrated to prevent very common clogging problems. Performance was characterized using microbeads (10 µm) and human cancer cells that were spiked in human blood. Design-I showed a capture efficiency of 96% for microbeads and 87% for cancer cells at 1 ml/min flow rate. An improved Design-II presented a higher capture efficiency of 100% for microbeads and 96% for cancer cells. Our method of utilizing various microfluidic functions of separation, bypass and capture has successfully guaranteed highly efficient separation of rare cells from biological fluids.
format	article
author	Dilip Venugopal Nanda Kasani Yariswamy Manjunath Guangfu Li Jussuf T. Kaifi Jae W. Kwon
author_facet	Dilip Venugopal Nanda Kasani Yariswamy Manjunath Guangfu Li Jussuf T. Kaifi Jae W. Kwon
author_sort	Dilip Venugopal
title	Clog-free high-throughput microfluidic cell isolation with multifunctional microposts
title_short	Clog-free high-throughput microfluidic cell isolation with multifunctional microposts
title_full	Clog-free high-throughput microfluidic cell isolation with multifunctional microposts
title_fullStr	Clog-free high-throughput microfluidic cell isolation with multifunctional microposts
title_full_unstemmed	Clog-free high-throughput microfluidic cell isolation with multifunctional microposts
title_sort	clog-free high-throughput microfluidic cell isolation with multifunctional microposts
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/17765ab8ef0a4ba5a09651028283e7a5
work_keys_str_mv	AT dilipvenugopal clogfreehighthroughputmicrofluidiccellisolationwithmultifunctionalmicroposts AT nandakasani clogfreehighthroughputmicrofluidiccellisolationwithmultifunctionalmicroposts AT yariswamymanjunath clogfreehighthroughputmicrofluidiccellisolationwithmultifunctionalmicroposts AT guangfuli clogfreehighthroughputmicrofluidiccellisolationwithmultifunctionalmicroposts AT jussuftkaifi clogfreehighthroughputmicrofluidiccellisolationwithmultifunctionalmicroposts AT jaewkwon clogfreehighthroughputmicrofluidiccellisolationwithmultifunctionalmicroposts
_version_	1718377372102688768

Clog-free high-throughput microfluidic cell isolation with multifunctional microposts

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