The brain microvascular endothelium supports T cell proliferation and has potential for alloantigen presentation.

Endothelial cells (EC) form the inner lining of blood vessels and are positioned between circulating lymphocytes and tissues. Hypotheses have formed that EC may act as antigen presenting cells based on the intimate interactions with T cells, which are seen in diseases like multiple sclerosis, cerebr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Julie Wheway, Stephanie Obeid, Pierre-Olivier Couraud, Valery Combes, Georges E R Grau
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/177d810117704c13b51a0c7a71b8d7ef
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:177d810117704c13b51a0c7a71b8d7ef
record_format	dspace
spelling	oai:doaj.org-article:177d810117704c13b51a0c7a71b8d7ef2021-11-18T08:02:20ZThe brain microvascular endothelium supports T cell proliferation and has potential for alloantigen presentation.1932-620310.1371/journal.pone.0052586https://doaj.org/article/177d810117704c13b51a0c7a71b8d7ef2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23320074/?tool=EBIhttps://doaj.org/toc/1932-6203Endothelial cells (EC) form the inner lining of blood vessels and are positioned between circulating lymphocytes and tissues. Hypotheses have formed that EC may act as antigen presenting cells based on the intimate interactions with T cells, which are seen in diseases like multiple sclerosis, cerebral malaria (CM) and viral neuropathologies. Here, we investigated how human brain microvascular EC (HBEC) interact with and support the proliferation of T cells. We found HBEC to express MHC II, CD40 and ICOSL, key molecules for antigen presentation and co-stimulation and to take up fluorescently labeled antigens via macropinocytosis. In co-cultures, we showed that HBEC support and promote the proliferation of CD4(+) and CD8(+) T cells, which both are key in CM pathogenesis, particularly following T cell receptor activation and co-stimulation. Our findings provide novel evidence that HBEC can trigger T cell activation, thereby providing a novel mechanism for neuroimmunological complications of infectious diseases.Julie WhewayStephanie ObeidPierre-Olivier CouraudValery CombesGeorges E R GrauPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e52586 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julie Wheway Stephanie Obeid Pierre-Olivier Couraud Valery Combes Georges E R Grau The brain microvascular endothelium supports T cell proliferation and has potential for alloantigen presentation.
description	Endothelial cells (EC) form the inner lining of blood vessels and are positioned between circulating lymphocytes and tissues. Hypotheses have formed that EC may act as antigen presenting cells based on the intimate interactions with T cells, which are seen in diseases like multiple sclerosis, cerebral malaria (CM) and viral neuropathologies. Here, we investigated how human brain microvascular EC (HBEC) interact with and support the proliferation of T cells. We found HBEC to express MHC II, CD40 and ICOSL, key molecules for antigen presentation and co-stimulation and to take up fluorescently labeled antigens via macropinocytosis. In co-cultures, we showed that HBEC support and promote the proliferation of CD4(+) and CD8(+) T cells, which both are key in CM pathogenesis, particularly following T cell receptor activation and co-stimulation. Our findings provide novel evidence that HBEC can trigger T cell activation, thereby providing a novel mechanism for neuroimmunological complications of infectious diseases.
format	article
author	Julie Wheway Stephanie Obeid Pierre-Olivier Couraud Valery Combes Georges E R Grau
author_facet	Julie Wheway Stephanie Obeid Pierre-Olivier Couraud Valery Combes Georges E R Grau
author_sort	Julie Wheway
title	The brain microvascular endothelium supports T cell proliferation and has potential for alloantigen presentation.
title_short	The brain microvascular endothelium supports T cell proliferation and has potential for alloantigen presentation.
title_full	The brain microvascular endothelium supports T cell proliferation and has potential for alloantigen presentation.
title_fullStr	The brain microvascular endothelium supports T cell proliferation and has potential for alloantigen presentation.
title_full_unstemmed	The brain microvascular endothelium supports T cell proliferation and has potential for alloantigen presentation.
title_sort	brain microvascular endothelium supports t cell proliferation and has potential for alloantigen presentation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/177d810117704c13b51a0c7a71b8d7ef
work_keys_str_mv	AT juliewheway thebrainmicrovascularendotheliumsupportstcellproliferationandhaspotentialforalloantigenpresentation AT stephanieobeid thebrainmicrovascularendotheliumsupportstcellproliferationandhaspotentialforalloantigenpresentation AT pierreoliviercouraud thebrainmicrovascularendotheliumsupportstcellproliferationandhaspotentialforalloantigenpresentation AT valerycombes thebrainmicrovascularendotheliumsupportstcellproliferationandhaspotentialforalloantigenpresentation AT georgesergrau thebrainmicrovascularendotheliumsupportstcellproliferationandhaspotentialforalloantigenpresentation AT juliewheway brainmicrovascularendotheliumsupportstcellproliferationandhaspotentialforalloantigenpresentation AT stephanieobeid brainmicrovascularendotheliumsupportstcellproliferationandhaspotentialforalloantigenpresentation AT pierreoliviercouraud brainmicrovascularendotheliumsupportstcellproliferationandhaspotentialforalloantigenpresentation AT valerycombes brainmicrovascularendotheliumsupportstcellproliferationandhaspotentialforalloantigenpresentation AT georgesergrau brainmicrovascularendotheliumsupportstcellproliferationandhaspotentialforalloantigenpresentation
_version_	1718422590468390912

The brain microvascular endothelium supports T cell proliferation and has potential for alloantigen presentation.

Ejemplares similares