TRFLP analysis for revealing the diversity of rice phyllosphere bacteria

Wiraswati SM, Wahyudi AT, Rusmana I, Nawangsih AA. 2018. TRFLP analysis for revealing the diversity of rice phyllosphere bacteria. Biodiversitas 19: 1743-1749. Phyllosphere environment of rice plant is usually inhabited by diverse bacteria which mostly contribute beneficial effects to the plant fitn...

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Autores principales:	SRI MARTINA WIRASWATI, ARIS TRI WAHYUDI, IMAN RUSMANA, ABDJAD ASIH NAWANGSIH
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	bacteria phyllosphere rice trflp Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/177e873944074dd1a361a4fb2ef16b9b
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spelling	oai:doaj.org-article:177e873944074dd1a361a4fb2ef16b9b2021-11-16T14:04:05ZTRFLP analysis for revealing the diversity of rice phyllosphere bacteria1412-033X2085-472210.13057/biodiv/d190521https://doaj.org/article/177e873944074dd1a361a4fb2ef16b9b2018-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2940https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Wiraswati SM, Wahyudi AT, Rusmana I, Nawangsih AA. 2018. TRFLP analysis for revealing the diversity of rice phyllosphere bacteria. Biodiversitas 19: 1743-1749. Phyllosphere environment of rice plant is usually inhabited by diverse bacteria which mostly contribute beneficial effects to the plant fitness. TRFLP method is a rapid and straightforward method to determine the bacterial diversity of many environments, including rice phyllosphere environment. This study aimed to analyze rice phyllosphere bacterial diversity of healthy rice plant cultivar Ciherang obtained from Sukabumi, Jasinga, and Situgede. The bacterial genomes were amplified and digested with two restriction enzymes, i.e., MspI and BstUI. The bacterial diversity (H’ index) and evenness (E index) were calculated from the peak value. From TRFs analysis, Betaproteobacteria and Pseudomonadales were dominantly found in nearly all samples with different relative abundance. In addition, Alphaproteobacteria and Gammaproteobacteria were also dominant in the several samples. The unique bacteria groups were inhabited in the sample from specific regions with certain growth phase. This finding informs us that the geographical factors might be more influent than the growth phase factor. Furthermore, the bacterial diversity and evenness of the metagenomic approach are higher than cultivation-dependent approach.SRI MARTINA WIRASWATIARIS TRI WAHYUDIIMAN RUSMANAABDJAD ASIH NAWANGSIHMBI & UNS SoloarticlebacteriaphyllospherericetrflpBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 5, Pp 1743-1749 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteria phyllosphere rice trflp Biology (General) QH301-705.5
spellingShingle	bacteria phyllosphere rice trflp Biology (General) QH301-705.5 SRI MARTINA WIRASWATI ARIS TRI WAHYUDI IMAN RUSMANA ABDJAD ASIH NAWANGSIH TRFLP analysis for revealing the diversity of rice phyllosphere bacteria
description	Wiraswati SM, Wahyudi AT, Rusmana I, Nawangsih AA. 2018. TRFLP analysis for revealing the diversity of rice phyllosphere bacteria. Biodiversitas 19: 1743-1749. Phyllosphere environment of rice plant is usually inhabited by diverse bacteria which mostly contribute beneficial effects to the plant fitness. TRFLP method is a rapid and straightforward method to determine the bacterial diversity of many environments, including rice phyllosphere environment. This study aimed to analyze rice phyllosphere bacterial diversity of healthy rice plant cultivar Ciherang obtained from Sukabumi, Jasinga, and Situgede. The bacterial genomes were amplified and digested with two restriction enzymes, i.e., MspI and BstUI. The bacterial diversity (H’ index) and evenness (E index) were calculated from the peak value. From TRFs analysis, Betaproteobacteria and Pseudomonadales were dominantly found in nearly all samples with different relative abundance. In addition, Alphaproteobacteria and Gammaproteobacteria were also dominant in the several samples. The unique bacteria groups were inhabited in the sample from specific regions with certain growth phase. This finding informs us that the geographical factors might be more influent than the growth phase factor. Furthermore, the bacterial diversity and evenness of the metagenomic approach are higher than cultivation-dependent approach.
format	article
author	SRI MARTINA WIRASWATI ARIS TRI WAHYUDI IMAN RUSMANA ABDJAD ASIH NAWANGSIH
author_facet	SRI MARTINA WIRASWATI ARIS TRI WAHYUDI IMAN RUSMANA ABDJAD ASIH NAWANGSIH
author_sort	SRI MARTINA WIRASWATI
title	TRFLP analysis for revealing the diversity of rice phyllosphere bacteria
title_short	TRFLP analysis for revealing the diversity of rice phyllosphere bacteria
title_full	TRFLP analysis for revealing the diversity of rice phyllosphere bacteria
title_fullStr	TRFLP analysis for revealing the diversity of rice phyllosphere bacteria
title_full_unstemmed	TRFLP analysis for revealing the diversity of rice phyllosphere bacteria
title_sort	trflp analysis for revealing the diversity of rice phyllosphere bacteria
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/177e873944074dd1a361a4fb2ef16b9b
work_keys_str_mv	AT srimartinawiraswati trflpanalysisforrevealingthediversityofricephyllospherebacteria AT aristriwahyudi trflpanalysisforrevealingthediversityofricephyllospherebacteria AT imanrusmana trflpanalysisforrevealingthediversityofricephyllospherebacteria AT abdjadasihnawangsih trflpanalysisforrevealingthediversityofricephyllospherebacteria
_version_	1718426405347262464

TRFLP analysis for revealing the diversity of rice phyllosphere bacteria

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