Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey

In this study, a sensitive monoclonal antibody (mAb) 3D5 against sparfloxacin (SPFX) was generated. Based on the mAb 3D5, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was developed for the detection of SPFX in honey. The half maximal inhibitory concentration and limit...

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Autores principales:	Jingjing Li, Aimin Zhi, Guochao Jia, Hui Nie, Lianzhong Ai
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	sparfloxacin monoclonal antibody ic-elisa ica strip honey Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/177eb9bd7eab4d3f8afbb544b70847c0
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spelling	oai:doaj.org-article:177eb9bd7eab4d3f8afbb544b70847c02021-11-26T11:19:46ZDevelopment of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey0954-01051465-344310.1080/09540105.2017.1364706https://doaj.org/article/177eb9bd7eab4d3f8afbb544b70847c02018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1364706https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443In this study, a sensitive monoclonal antibody (mAb) 3D5 against sparfloxacin (SPFX) was generated. Based on the mAb 3D5, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was developed for the detection of SPFX in honey. The half maximal inhibitory concentration and limit of detection for the ic-ELISA method were 0.12 and 0.02 ng/mL, respectively. The experiments showed a negligible cross-reaction with other drugs. The average recovery rates for spiked SPFX honey extracts ranged from 90% to 101%, indicating an accepted accuracy. Furthermore, a lateral-flow immunochromatographic assay strip method with a cut-off value of 2 ng/mL was developed. Therefore, both of the developed methods are suitable for future use as rapid screening methods to detect and control the content of SPFX residues in honey samples.Jingjing LiAimin ZhiGuochao JiaHui NieLianzhong AiTaylor & Francis Grouparticlesparfloxacinmonoclonal antibodyic-elisaica striphoneyAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 147-158 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	sparfloxacin monoclonal antibody ic-elisa ica strip honey Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	sparfloxacin monoclonal antibody ic-elisa ica strip honey Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Jingjing Li Aimin Zhi Guochao Jia Hui Nie Lianzhong Ai Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey
description	In this study, a sensitive monoclonal antibody (mAb) 3D5 against sparfloxacin (SPFX) was generated. Based on the mAb 3D5, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was developed for the detection of SPFX in honey. The half maximal inhibitory concentration and limit of detection for the ic-ELISA method were 0.12 and 0.02 ng/mL, respectively. The experiments showed a negligible cross-reaction with other drugs. The average recovery rates for spiked SPFX honey extracts ranged from 90% to 101%, indicating an accepted accuracy. Furthermore, a lateral-flow immunochromatographic assay strip method with a cut-off value of 2 ng/mL was developed. Therefore, both of the developed methods are suitable for future use as rapid screening methods to detect and control the content of SPFX residues in honey samples.
format	article
author	Jingjing Li Aimin Zhi Guochao Jia Hui Nie Lianzhong Ai
author_facet	Jingjing Li Aimin Zhi Guochao Jia Hui Nie Lianzhong Ai
author_sort	Jingjing Li
title	Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey
title_short	Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey
title_full	Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey
title_fullStr	Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey
title_full_unstemmed	Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey
title_sort	development of an ic-elisa and immunochromatographic strip for detection of sparfloxacin in honey
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/177eb9bd7eab4d3f8afbb544b70847c0
work_keys_str_mv	AT jingjingli developmentofanicelisaandimmunochromatographicstripfordetectionofsparfloxacininhoney AT aiminzhi developmentofanicelisaandimmunochromatographicstripfordetectionofsparfloxacininhoney AT guochaojia developmentofanicelisaandimmunochromatographicstripfordetectionofsparfloxacininhoney AT huinie developmentofanicelisaandimmunochromatographicstripfordetectionofsparfloxacininhoney AT lianzhongai developmentofanicelisaandimmunochromatographicstripfordetectionofsparfloxacininhoney
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Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey

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