Screening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana

Abstract Endophytic fungi with the ability to produce plant based secondary metabolites are a potential alternative for producing the host plant metabolite and to prevent natural plants from extinction. To isolate a high metabolite yielding endophytic strain from plants, hundreds of endophytic strai...

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Autores principales:	I. A. H. Khwajah Mohinudeen, Saumya Pandey, Hemalatha Kanniyappan, Vignesh Muthuvijayan, Smita Srivastava
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/177f92a5f49942e2a7465bbfd9902b9e
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spelling	oai:doaj.org-article:177f92a5f49942e2a7465bbfd9902b9e2021-12-02T16:53:02ZScreening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana10.1038/s41598-021-90778-32045-2322https://doaj.org/article/177f92a5f49942e2a7465bbfd9902b9e2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90778-3https://doaj.org/toc/2045-2322Abstract Endophytic fungi with the ability to produce plant based secondary metabolites are a potential alternative for producing the host plant metabolite and to prevent natural plants from extinction. To isolate a high metabolite yielding endophytic strain from plants, hundreds of endophytic strains are screened and tested for product yield separately under axenic state, before shortlisting the potential endophyte, which involves huge time consumption. In this study, strategies for screening and selection of high camptothecin yielding endophytes from their natural habitat were proposed. A correlation was built between the camptothecin yield in the explants and the endophytes isolated from them. In addition, camptothecin yield was compared between the endophytes isolated from young and matured plants. Further, camptothecin producers and non-producers strains were compared for their tolerance toward camptothecin. The study indicates that high camptothecin yielding endophytes were isolated from high yielding explants and younger plants and they were more tolerant to camptothecin in comparison to non-camptothecin yielding endophytes. Thus, choosing a young and high yielding explant for endophyte isolation, and use of camptothecin as a selective agent in the growth medium, can be instrumental in screening and selection of high camptothecin yielding endophytes from nature in relatively less time.I. A. H. Khwajah MohinudeenSaumya PandeyHemalatha KanniyappanVignesh MuthuvijayanSmita SrivastavaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q I. A. H. Khwajah Mohinudeen Saumya Pandey Hemalatha Kanniyappan Vignesh Muthuvijayan Smita Srivastava Screening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana
description	Abstract Endophytic fungi with the ability to produce plant based secondary metabolites are a potential alternative for producing the host plant metabolite and to prevent natural plants from extinction. To isolate a high metabolite yielding endophytic strain from plants, hundreds of endophytic strains are screened and tested for product yield separately under axenic state, before shortlisting the potential endophyte, which involves huge time consumption. In this study, strategies for screening and selection of high camptothecin yielding endophytes from their natural habitat were proposed. A correlation was built between the camptothecin yield in the explants and the endophytes isolated from them. In addition, camptothecin yield was compared between the endophytes isolated from young and matured plants. Further, camptothecin producers and non-producers strains were compared for their tolerance toward camptothecin. The study indicates that high camptothecin yielding endophytes were isolated from high yielding explants and younger plants and they were more tolerant to camptothecin in comparison to non-camptothecin yielding endophytes. Thus, choosing a young and high yielding explant for endophyte isolation, and use of camptothecin as a selective agent in the growth medium, can be instrumental in screening and selection of high camptothecin yielding endophytes from nature in relatively less time.
format	article
author	I. A. H. Khwajah Mohinudeen Saumya Pandey Hemalatha Kanniyappan Vignesh Muthuvijayan Smita Srivastava
author_facet	I. A. H. Khwajah Mohinudeen Saumya Pandey Hemalatha Kanniyappan Vignesh Muthuvijayan Smita Srivastava
author_sort	I. A. H. Khwajah Mohinudeen
title	Screening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana
title_short	Screening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana
title_full	Screening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana
title_fullStr	Screening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana
title_full_unstemmed	Screening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana
title_sort	screening and selection of camptothecin producing endophytes from nothapodytes nimmoniana
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/177f92a5f49942e2a7465bbfd9902b9e
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Screening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana

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