The genes crucial to carotenoid metabolism under elevated CO2 levels in carrot (Daucus carota L.)

Abstract The CO2 saturation point can reach as high as 1819 μmol· mol−1 in carrot (Daucus carota L.). In recent years, carrot has been cultivated in out-of-season greenhouses, but the molecular mechanism of CO2 enrichment has been ignored, and this is a missed opportunity to gain a comprehensive und...

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Autores principales:	Hongxia Song, Qiang Lu, Leiping Hou, Meilan Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1791bc9af6b1408f957f1bbef8065b35
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spelling	oai:doaj.org-article:1791bc9af6b1408f957f1bbef8065b352021-12-02T17:30:40ZThe genes crucial to carotenoid metabolism under elevated CO2 levels in carrot (Daucus carota L.)10.1038/s41598-021-91522-72045-2322https://doaj.org/article/1791bc9af6b1408f957f1bbef8065b352021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91522-7https://doaj.org/toc/2045-2322Abstract The CO2 saturation point can reach as high as 1819 μmol· mol−1 in carrot (Daucus carota L.). In recent years, carrot has been cultivated in out-of-season greenhouses, but the molecular mechanism of CO2 enrichment has been ignored, and this is a missed opportunity to gain a comprehensive understanding of this important process. In this study, it was found that CO2 enrichment increased the aboveground and belowground biomasses and greatly increased the carotenoid contents. Twenty genes related to carotenoids were discovered in 482 differentially expressed genes (DEGs) through RNA sequencing (RNA-Seq.). These genes were involved in either carotenoid biosynthesis or the composition of the photosystem membrane proteins, most of which were upregulated. We suspected that these genes were directly related to quality improvement and increases in biomass under CO2 enrichment in carrot. As such, β-carotene hydroxylase activity in carotenoid metabolism and the expression levels of coded genes were determined and analysed, and the results were consistent with the observed change in carotenoid content. These results illustrate the molecular mechanism by which the increase in carotenoid content after CO2 enrichment leads to the improvement of quality and biological yield. Our findings have important theoretical and practical significance.Hongxia SongQiang LuLeiping HouMeilan LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hongxia Song Qiang Lu Leiping Hou Meilan Li The genes crucial to carotenoid metabolism under elevated CO2 levels in carrot (Daucus carota L.)
description	Abstract The CO2 saturation point can reach as high as 1819 μmol· mol−1 in carrot (Daucus carota L.). In recent years, carrot has been cultivated in out-of-season greenhouses, but the molecular mechanism of CO2 enrichment has been ignored, and this is a missed opportunity to gain a comprehensive understanding of this important process. In this study, it was found that CO2 enrichment increased the aboveground and belowground biomasses and greatly increased the carotenoid contents. Twenty genes related to carotenoids were discovered in 482 differentially expressed genes (DEGs) through RNA sequencing (RNA-Seq.). These genes were involved in either carotenoid biosynthesis or the composition of the photosystem membrane proteins, most of which were upregulated. We suspected that these genes were directly related to quality improvement and increases in biomass under CO2 enrichment in carrot. As such, β-carotene hydroxylase activity in carotenoid metabolism and the expression levels of coded genes were determined and analysed, and the results were consistent with the observed change in carotenoid content. These results illustrate the molecular mechanism by which the increase in carotenoid content after CO2 enrichment leads to the improvement of quality and biological yield. Our findings have important theoretical and practical significance.
format	article
author	Hongxia Song Qiang Lu Leiping Hou Meilan Li
author_facet	Hongxia Song Qiang Lu Leiping Hou Meilan Li
author_sort	Hongxia Song
title	The genes crucial to carotenoid metabolism under elevated CO2 levels in carrot (Daucus carota L.)
title_short	The genes crucial to carotenoid metabolism under elevated CO2 levels in carrot (Daucus carota L.)
title_full	The genes crucial to carotenoid metabolism under elevated CO2 levels in carrot (Daucus carota L.)
title_fullStr	The genes crucial to carotenoid metabolism under elevated CO2 levels in carrot (Daucus carota L.)
title_full_unstemmed	The genes crucial to carotenoid metabolism under elevated CO2 levels in carrot (Daucus carota L.)
title_sort	genes crucial to carotenoid metabolism under elevated co2 levels in carrot (daucus carota l.)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1791bc9af6b1408f957f1bbef8065b35
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The genes crucial to carotenoid metabolism under elevated CO2 levels in carrot (Daucus carota L.)

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